Key features and details
- Rabbit polyclonal to EZH1 - N-terminal
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Product nameAnti-EZH1 antibody - N-terminal
See all EZH1 primary antibodies
DescriptionRabbit polyclonal to EZH1 - N-terminal
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human EZH1 aa 180-230 (N terminal). The exact sequence is proprietary.
Database link: Q92800
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.2
Preservative: 0.02% Sodium azide
Constituent: 99% PBS
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab176115 is affinity chromatography purified via peptide column.
Our Abpromise guarantee covers the use of ab176115 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 85 kDa.|
|IHC-P||Use a concentration of 5 µg/ml.|
FunctionPolycomb group (PcG) protein. Catalytic subunit of the PRC2/EED-EZH1 complex, which methylates 'Lys-27' of histone H3, leading to transcriptional repression of the affected target gene. Able to mono-, di- and trimethylate 'Lys-27' of histone H3 to form H3K27me1, H3K27me2 and H3K27me3, respectively. Required for embryonic stem cell derivation and self-renewal, suggesting that it is involved in safeguarding embryonic stem cell identity. Compared to EZH1-containing complexes, it is less abundant in embryonic stem cells, has weak methyltransferase activity and plays a less critical role in forming H3K27me3, which is required for embryonic stem cell identity and proper differentiation.
Sequence similaritiesBelongs to the histone-lysine methyltransferase family. EZ subfamily.
Contains 1 SET domain.
Cellular localizationNucleus. Colocalizes with trimethylated 'Lys-27' of histone H3.
- Information by UniProt
- Enhancer of zeste homolog 1 (Drosophila) antibody
- Enhancer of zeste homolog 1 antibody
- ENX-2 antibody
Immunohistochemical analysis of Human lung tissue labeling EZH1 with ab176115 at 5 μg/ml dilution.
Lane 1 : Anti-EZH1 antibody - N-terminal (ab176115) at 1 µg/ml
Lane 2 : Anti-EZH1 antibody - N-terminal (ab176115) at 2 µg/ml
All lanes : mouse lung tissue lysate
Lysates/proteins at 15 µg per lane.
Developed using the ECL technique.
Predicted band size: 85 kDa
ab176115 has been referenced in 2 publications.
- Agarwal P et al. Mesenchymal Niche-Specific Expression of Cxcl12 Controls Quiescence of Treatment-Resistant Leukemia Stem Cells. Cell Stem Cell 24:769-784.e6 (2019). PubMed: 30905620
- Li J et al. EZH2-mediated H3K27 trimethylation mediates neurodegeneration in ataxia-telangiectasia. Nat Neurosci 16:1745-53 (2013). PubMed: 24162653