Key features and details
- Rabbit polyclonal to Ezrin / Radixin / Moesin
- Suitable for: IHC-P, WB
- Reacts with: Human, Pig
- Isotype: IgG
Product nameAnti-Ezrin / Radixin / Moesin antibody
See all Ezrin / Radixin / Moesin primary antibodies
DescriptionRabbit polyclonal to Ezrin / Radixin / Moesin
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Human, Pig
Predicted to work with: Mouse, Rat
Recombinant fragment (His-T7-tag) corresponding to Human Ezrin/ Radixin/ Moesin aa 5-295. (Expressed in E.coli)
INVRVTTMDAELEFAIQPNTTGKQLFDQVVKTVGLREVWFFGLQYVDSKG YSTWLKLNKKVTQQDVKKENPLQFKFRAKFFPEDVSEELIQEITQRLFFL QVKEAILNDEIYCPPETAVLLASYAVQAKYGDYNKEIHKPGYLANDRLLP QRVLEQHKLTKEQWEERIQNWHEEHRGMLREDSMMEYLKIAQDLEMYGVN YFEIKNKKGTELWLGVDALGLNIYEHDDKLTPKIGFPWSEIRNISFNDKK FVIKPIDKKAPDFVFYAPRLRINKRILALCM
Database link: P35241
- IHC-P: Human liver cancer and human kidney tissues. WB: Recombinant human Ezrin / Radixin / Moesin protein; Pig liver and kidney tissues; HeLa and HEK-293T cells.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Purification notesAntigen-specific affinity chromatography followed by Protein A affinity chromatography.
Our Abpromise guarantee covers the use of ab231642 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 5 - 20 µg/ml.|
|WB||Use a concentration of 0.5 - 2 µg/ml.|
RelevanceEzrin is a cytoplasmic peripheral membrane protein and functions as a protein-tyrosine kinase substrate in microvilli. As a member of the ERM protein family, this protein serves as an intermediate between the plasma membrane and the actin cytoskeleton. This protein plays a key role in cell surface structure adhesion, migration and organization, and it has been implicated in various human cancers. A pseudogene located on chromosome 3 has been identified for this gene. Alternatively spliced variants have also been described for this gene. Radixin is a cytoskeletal protein that may be important in linking actin to the plasma membrane. It is highly similar in sequence to both ezrin and moesin. The radixin gene has been localized by fluorescence in situ hybridization to 11q23. A truncated version representing a pseudogene (RDXP2) was assigned to Xp21.3. Another pseudogene that seemed to lack introns (RDXP1) was mapped to 11p by Southern and PCR analyses. Moesin is a member of the ERM family which includes ezrin and radixin. ERM proteins appear to function as cross-linkers between plasma membranes and actin-based cytoskeletons. Moesin is localized to filopodia and other membranous protrusions that are important for cell-cell recognition and signaling and for cell movement.
Cellular localizationCell Membrane, Cytoskeleton and Cytoplasmic
- CVIL antibody
- CVL antibody
- Cytovillin 2 antibody
All lanes : Anti-Ezrin / Radixin / Moesin antibody (ab231642) at 1 µg/ml
Lane 1 : Pig liver tissue lysate.
Lane 2 : Pig kidney tissue lysate.
Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate
Lane 4 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate
All lanes : HRP-Linked Guinea pig anti-Rabbit Ab at 1/2000 dilution
Developed using the ECL technique.
Anti-Ezrin / Radixin / Moesin antibody (ab231642) at 2 µg/ml + Recombinant human Ezrin / Radixin / Moesin protein.
Developed using the ECL technique.
Formalin-fixed, paraffin-embedded human kidney tissue stained for Ezrin / Radixin / Moesin using ab231642 at 20 µg/mL in immunohistochemical analysis.
Formalin-fixed, paraffin-embedded human liver cancer tissue stained for Ezrin / Radixin / Moesin using ab231642 at 20 µg/mL in immunohistochemical analysis.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab231642 has not yet been referenced specifically in any publications.