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Recombinant fragment, corresponding to amino acids 362-585 of Human Ezrin.
Our Abpromise guarantee covers the use of ab4069 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-FoFr||Use at an assay dependent concentration. PubMed: 22427845|
|WB||1/100 - 1/750. Predicted molecular weight: 69 kDa.|
|ICC/IF||Use at an assay dependent concentration. PubMed: 17311105|
|IHC-P||1/50 - 1/100.|
|IHC-Fr||1/50 - 1/100.|
|Flow Cyt||Use 1µg for 106 cells.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
Lanes 1 - 5: Merged signal (red and green). Green - ab4069 observed at 81 kDa. Red - loading control, ab181602, observed at 37 kDa.
ab4069 was shown to specifically react with Ezrin in wild-type HAP1 cells as signal was lost in EZR (Ezrin) knockout cells. Wild-type and EZR (Ezrin) knockout samples were subjected to SDS-PAGE. Ab4069 and ab181602 (Rabbit anti-GAPDH loading control) were incubated overnight at 4°C at 1 μg/ml and 1/20000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed ab216772 and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed ab216777 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
ab4069 at 1/100 dilution staining human HeLa cells by ICC/IF. The cells were formaldehyde fixed and blocked with BSA/goat serum prior to incubation with the antibody for 30 minutes. An Alexa-Fluor ® 568 conjugated goat anti-mouse IgG was used as the secondary.
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