Anti-Ezrin antibody [6F1A9] (ab205381)
Key features and details
- Mouse monoclonal [6F1A9] to Ezrin
- Suitable for: WB, Flow Cyt, ICC/IF
- Reacts with: Mouse, Human, African green monkey
- Isotype: IgG1
Overview
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Product name
Anti-Ezrin antibody [6F1A9]
See all Ezrin primary antibodies -
Description
Mouse monoclonal [6F1A9] to Ezrin -
Host species
Mouse -
Tested applications
Suitable for: WB, Flow Cyt, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Human, African green monkey
Predicted to work with: Rat, Rabbit, Cow -
Immunogen
Recombinant fragment corresponding to Human Ezrin aa 250-500. Expressed in E. Coli.
Database link: P15311 -
Positive control
- Human Ezrin (AA: 292-464) recombinant protein; Ezrin (AA: 292-464)-hIgGFc transfected HEK293 cell lysate; MCF7, HeLa, A431, HEK293, SK N SH, Jurkat, HepG2, NIH 3T3 and COS7 cell lysates; A549 cells; MCF7 cells and HeLa cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituent: 99% PBS -
Concentration information loading...
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Purity
Protein G purified -
Purification notes
Purified from tissue culture supernatant. -
Clonality
Monoclonal -
Clone number
6F1A9 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
- HeLa whole cell lysate (ab150035)
- Hep G2 whole cell lysate (ab166833)
- MCF7 whole cell lysate (ab29537)
- HeLa whole cell lysate (ab29545)
- Jurkat whole cell lysate (ab30128)
- A-431 whole cell lysate (ab30132)
- SK-N-SH whole cell lysate (ab3956)
- NIH/3T3 whole cell lysate (ab7179)
- Jurkat whole cell lysate (ab7899)
- HEK-293 whole cell lysate (ab7902)
- A-431 whole cell lysate (ab7909)
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab205381 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/2000. Predicted molecular weight: 69 kDa.
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Flow Cyt |
1/200 - 1/400.
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ICC/IF |
1/100 - 1/400.
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Notes |
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WB
1/500 - 1/2000. Predicted molecular weight: 69 kDa. |
Flow Cyt
1/200 - 1/400. |
ICC/IF
1/100 - 1/400. |
Target
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Function
Probably involved in connections of major cytoskeletal structures to the plasma membrane. In epithelial cells, required for the formation of microvilli and membrane ruffles on the apical pole. Along with PLEKHG6, required for normal macropinocytosis. -
Tissue specificity
Expressed in cerebral cortex, basal ganglia, hippocampus, hypophysis, and optic nerve. Weakly expressed in brain stem and diencephalon. Stronger expression was detected in gray matter of frontal lobe compared to white matter (at protein level). Component of the microvilli of intestinal epithelial cells. Preferentially expressed in astrocytes of hippocampus, frontal cortex, thalamus, parahippocampal cortex, amygdala, insula, and corpus callosum. Not detected in neurons in most tissues studied. -
Sequence similarities
Contains 1 FERM domain. -
Developmental stage
Very strong staining is detected in the Purkinje cell layer and in part of the molecular layer of the infant brain compared to adult brain. -
Post-translational
modificationsPhosphorylated by tyrosine-protein kinases. -
Cellular localization
Apical cell membrane. Cell projection. Cell projection > microvillus membrane. Cell projection > ruffle membrane. Cytoplasm > cell cortex. Cytoplasm > cytoskeleton. Localization to the apical membrane of parietal cells depends on the interaction with MPP5. Localizes to cell extensions and peripheral processes of astrocytes (By similarity). Microvillar peripheral membrane protein. - Information by UniProt
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Database links
- Entrez Gene: 281574 Cow
- Entrez Gene: 7430 Human
- Entrez Gene: 22350 Mouse
- Entrez Gene: 100008846 Rabbit
- Entrez Gene: 54319 Rat
- Omim: 123900 Human
- SwissProt: P31976 Cow
- SwissProt: P15311 Human
see all -
Alternative names
- Villin 2 ezrin antibody
- CVIL antibody
- CVL antibody
see all
Images
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Anti-Ezrin antibody [6F1A9] (ab205381) at 1/500 dilution + Human Ezrin (AA: 292-464) recombinant protein.
Predicted band size: 69 kDaExpected MW is 47.1 kDa
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All lanes : Anti-Ezrin antibody [6F1A9] (ab205381) at 1/500 dilution
Lane 1 : Non-transfected HEK293 cell lysate.
Lane 2 : Ezrin (AA: 292-464)-hIgGFc transfected HEK293 cell lysate.
Predicted band size: 69 kDa -
All lanes : Anti-Ezrin antibody [6F1A9] (ab205381) at 1/500 dilution
Lane 1 : MCF7 cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : A431 cell lysate
Lane 4 : HEK293 cell lysate
Lane 5 : SK N SH cell lysate
Lane 6 : Jurkat cell lysate
Lane 7 : HepG2 cell lysate
Lane 8 : NIH 3T3 cell lysate
Lane 9 : COS-7 cell lysate
Predicted band size: 69 kDa -
Immunofluorescent analysis of A549 cells labeling Ezrin using ab205381 at 1/100 dilution (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.
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Flow cytometric analysis of MCF7 cells labeling Ezrin using ab205381 at 1/200 dilution (green) and negative control (red).
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Flow cytometric analysis of HeLa cells labeling Ezrin using ab205381 at 1/200 dilution (green) and negative control (red).
Protocols
Datasheets and documents
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Datasheet download
References (0)
ab205381 has not yet been referenced specifically in any publications.