Western blot abreview for Anti-Ezrin antibody [EP886Y]

Good
Abreviews
Application
Western blot
Sample
Human Cell lysate - whole cell (Human brain)
Loading amount
10 µg
Specification
Human brain
Treatment
Lysis buffer (urea/thiourea)
Gel Running Conditions
Reduced Denaturing (4-20%)
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: RT°C

Other product details

Dilution
1/5000
Incubation time
24 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: Blocking buffer

Secondary antibody

Name
Non-Abcam antibody was used: anti Rabbit HRP
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/7500

Detection

Detection method
ECL+
Exposure
30 second(s)
Bands
Specific: 85 kDa

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Submitted Feb 03 2010

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