Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Ezrin antibody [EP886Y]

Good
Abreviews
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (brain cortex)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: EDTA, pH9, 100C 20 min
Permeabilization
No
Specification
brain cortex
Blocking step
H2O2 as blocking agent for 10 minute(s) · Concentration: 3% · Temperature: 25°C
Fixative
Formaldehyde

Other product details

Incubation time
20 minute(s) · Temperature: 25°C · Diluent: Dako diluent
Dilution
1/100

Secondary antibody

Name
Non-Abcam antibody was used: Leica Refine anti-mouse/rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: HRP polymer

Additional data

Additional Notes
various pretreatment regimens tested EDTA gave best signal to background IHC procedure automated on Leica Bond III

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Submitted Oct 16 2015

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