• Product name

    Anti-Ezrin (phospho T567) antibody
    See all Ezrin primary antibodies
  • Description

    Rabbit polyclonal to Ezrin (phospho T567)
  • Host species

  • Specificity

    This antibody detects endogenous levels of Ezrin only when phosphorylated at Threonine 567. This antibody will also react with Moesin at Threonine 577 and Radixin at Threonine 564.
  • Tested applications

    Suitable for: WB, ELISA, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Ssynthesized phosphopeptide derived from human Ezrin around the phosphorylation site of threonine 567 (Y-K-TP-L-R)

  • Positive control

    • Extracts from A431 cells.



Our Abpromise guarantee covers the use of ab47293 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 69 kDa (predicted molecular weight: 69 kDa).
ELISA 1/20000.
IHC-P Use a concentration of 1 µg/ml.


  • Function

    Probably involved in connections of major cytoskeletal structures to the plasma membrane. In epithelial cells, required for the formation of microvilli and membrane ruffles on the apical pole. Along with PLEKHG6, required for normal macropinocytosis.
  • Tissue specificity

    Expressed in cerebral cortex, basal ganglia, hippocampus, hypophysis, and optic nerve. Weakly expressed in brain stem and diencephalon. Stronger expression was detected in gray matter of frontal lobe compared to white matter (at protein level). Component of the microvilli of intestinal epithelial cells. Preferentially expressed in astrocytes of hippocampus, frontal cortex, thalamus, parahippocampal cortex, amygdala, insula, and corpus callosum. Not detected in neurons in most tissues studied.
  • Sequence similarities

    Contains 1 FERM domain.
  • Developmental stage

    Very strong staining is detected in the Purkinje cell layer and in part of the molecular layer of the infant brain compared to adult brain.
  • Post-translational

    Phosphorylated by tyrosine-protein kinases.
  • Cellular localization

    Apical cell membrane. Cell projection. Cell projection > microvillus membrane. Cell projection > ruffle membrane. Cytoplasm > cell cortex. Cytoplasm > cytoskeleton. Localization to the apical membrane of parietal cells depends on the interaction with MPP5. Localizes to cell extensions and peripheral processes of astrocytes (By similarity). Microvillar peripheral membrane protein.
  • Information by UniProt
  • Database links

  • Alternative names

    • Villin 2 ezrin antibody
    • CVIL antibody
    • CVL antibody
    • Cytovillin 2 antibody
    • Cytovillin antibody
    • DKFZp762H157 antibody
    • Epididymis secretory protein Li 105 antibody
    • EZR antibody
    • EZRI_HUMAN antibody
    • Ezrin antibody
    • FLJ26216 antibody
    • HEL S 105 antibody
    • MGC1584 antibody
    • p81 antibody
    • VIL 2 antibody
    • VIL2 antibody
    • Villin 2 (ezrin) antibody
    • Villin 2 antibody
    • Villin-2 antibody
    • Villin2 antibody
    see all


  • All lanes : Anti-Ezrin (phospho T567) antibody (ab47293)

    Lane 1 : EGF treated A431 cells, no added peptide
    Lane 2 : Untreated A431 cells, no added peptide

    Predicted band size: 69 kDa
    Observed band size: 69 kDa

  • Ab47293 staining human normal placenta. Staining is localised to apical membrane and cytoplasmic compartiment.
    Left panel: with primary antibody at 1 ug/ml. Right panel: isotype control.
    Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffers EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may b
  • All lanes : Anti-Ezrin (phospho T567) antibody (ab47293)

    Lane 1 : Jurkat cell lysate with PMA (125 ng/ml, 30 minutes)
    Lane 2 : Jurkat cell lysate

    Predicted band size: 69 kDa
    Observed band size: 69 kDa
    Additional bands at: 68 kDa (possible cross reactivity)

    The lower band is likely to be Moesin, which has a homologous immunogenic region.


This product has been referenced in:

  • Zhang X  et al. Fasudil increases temozolomide sensitivity and suppresses temozolomide-resistant glioma growth via inhibiting ROCK2/ABCG2. Cell Death Dis 9:190 (2018). Read more (PubMed: 29416017) »
  • Liu P  et al. Ezrin/NF-?B Pathway Regulates EGF-induced Epithelial-Mesenchymal Transition (EMT), Metastasis, and Progression of Osteosarcoma. Med Sci Monit 24:2098-2108 (2018). WB . Read more (PubMed: 29628496) »
See all 10 Publications for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Western blot
Dog Cell lysate - whole cell (canine osteosarcoma cells)
Gel Running Conditions
Reduced Denaturing (10)
Loading amount
20 µg
canine osteosarcoma cells
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

Abcam user community

Verified customer

Submitted Apr 17 2019

Western blot
Caenorhabditis elegans Tissue lysate - whole (whole worm lysates)
Gel Running Conditions
Non-reduced Denaturing (10% T gel concentration)
Loading amount
30000 cells
whole worm lysates
Blocking step
Roti Block as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 20°C

Abcam user community

Verified customer

Submitted Jun 20 2017

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