Anti-Ezrin (phospho Y478) antibody (ab79256)
Key features and details
- Rabbit polyclonal to Ezrin (phospho Y478)
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Ezrin (phospho Y478) antibody
See all Ezrin primary antibodies -
Description
Rabbit polyclonal to Ezrin (phospho Y478) -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide corresponding to Human Ezrin (phospho Y478).
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Positive control
- Extracts form HeLa cells treated with Na3VO4 (0.3nM, 40mins).
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
Without Mg2+ and Ca2+ -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
Purified from rabbit antiserum by affinity chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab79256 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/1000. Predicted molecular weight: 69 kDa.
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Notes |
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WB
1/500 - 1/1000. Predicted molecular weight: 69 kDa. |
Target
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Function
Probably involved in connections of major cytoskeletal structures to the plasma membrane. In epithelial cells, required for the formation of microvilli and membrane ruffles on the apical pole. Along with PLEKHG6, required for normal macropinocytosis. -
Tissue specificity
Expressed in cerebral cortex, basal ganglia, hippocampus, hypophysis, and optic nerve. Weakly expressed in brain stem and diencephalon. Stronger expression was detected in gray matter of frontal lobe compared to white matter (at protein level). Component of the microvilli of intestinal epithelial cells. Preferentially expressed in astrocytes of hippocampus, frontal cortex, thalamus, parahippocampal cortex, amygdala, insula, and corpus callosum. Not detected in neurons in most tissues studied. -
Sequence similarities
Contains 1 FERM domain. -
Developmental stage
Very strong staining is detected in the Purkinje cell layer and in part of the molecular layer of the infant brain compared to adult brain. -
Post-translational
modificationsPhosphorylated by tyrosine-protein kinases. -
Cellular localization
Apical cell membrane. Cell projection. Cell projection > microvillus membrane. Cell projection > ruffle membrane. Cytoplasm > cell cortex. Cytoplasm > cytoskeleton. Localization to the apical membrane of parietal cells depends on the interaction with MPP5. Localizes to cell extensions and peripheral processes of astrocytes (By similarity). Microvillar peripheral membrane protein. - Information by UniProt
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Database links
- Entrez Gene: 7430 Human
- Entrez Gene: 22350 Mouse
- Entrez Gene: 54319 Rat
- Omim: 123900 Human
- SwissProt: P15311 Human
- SwissProt: P26040 Mouse
- SwissProt: P31977 Rat
- Unigene: 487027 Human
see all -
Alternative names
- Villin 2 ezrin antibody
- CVIL antibody
- CVL antibody
see all
Images
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All lanes : Anti-Ezrin (phospho Y478) antibody (ab79256) at 1/500 dilution
Lane 1 : K562 cell lysate treated with Na3VO4 (0.3nM,
40mins)
Lane 2 : K562 cell lysate treated with Na3VO4 (0.3nM,
40mins) with immunizing phosphopeptide at 10 µg
Lysates/proteins at 30 µg per lane.
Predicted band size: 69 kDa
Observed band size: 69 kDa
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab79256 has been referenced in 1 publication.
- Liu P et al. Ezrin/NF-?B Pathway Regulates EGF-induced Epithelial-Mesenchymal Transition (EMT), Metastasis, and Progression of Osteosarcoma. Med Sci Monit 24:2098-2108 (2018). WB . PubMed: 29628496