Anti-Ezrin / Radixin / Moesin antibody (ab118572)
Key features and details
- Rabbit polyclonal to Ezrin / Radixin / Moesin
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
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Overview
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Product name
Anti-Ezrin / Radixin / Moesin antibody
See all Ezrin / Radixin / Moesin primary antibodies -
Description
Rabbit polyclonal to Ezrin / Radixin / Moesin -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Synthetic peptide conjugated to KLH, corresponding to a region near the C terminus of Human Ezrin/ Radixin/ Moesin.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C or -80°C. Avoid freeze / thaw cycle. Store undiluted. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 0.88% Sodium chloride, 50% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab118572 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/1000. Predicted molecular weight: 68 kDa.
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IHC-P |
Use a concentration of 10 µg/ml.
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Notes |
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WB
1/500 - 1/1000. Predicted molecular weight: 68 kDa. |
IHC-P
Use a concentration of 10 µg/ml. |
Target
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Relevance
Ezrin is a cytoplasmic peripheral membrane protein and functions as a protein-tyrosine kinase substrate in microvilli. As a member of the ERM protein family, this protein serves as an intermediate between the plasma membrane and the actin cytoskeleton. This protein plays a key role in cell surface structure adhesion, migration and organization, and it has been implicated in various human cancers. A pseudogene located on chromosome 3 has been identified for this gene. Alternatively spliced variants have also been described for this gene. Radixin is a cytoskeletal protein that may be important in linking actin to the plasma membrane. It is highly similar in sequence to both ezrin and moesin. The radixin gene has been localized by fluorescence in situ hybridization to 11q23. A truncated version representing a pseudogene (RDXP2) was assigned to Xp21.3. Another pseudogene that seemed to lack introns (RDXP1) was mapped to 11p by Southern and PCR analyses. Moesin is a member of the ERM family which includes ezrin and radixin. ERM proteins appear to function as cross-linkers between plasma membranes and actin-based cytoskeletons. Moesin is localized to filopodia and other membranous protrusions that are important for cell-cell recognition and signaling and for cell movement. -
Cellular localization
Cell Membrane, Cytoskeleton and Cytoplasmic -
Database links
- Entrez Gene: 4478 Human
- Entrez Gene: 5962 Human
- Entrez Gene: 7430 Human
- Entrez Gene: 17698 Mouse
- Entrez Gene: 19684 Mouse
- Entrez Gene: 22350 Mouse
- Omim: 123900 Human
- Omim: 179410 Human
see all -
Alternative names
- CVIL antibody
- CVL antibody
- Cytovillin 2 antibody
see all
Images
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ab118572, at 10 µg/ml, staining Ezrin / Radixin / Moesin in formalin-fixed, paraffin-embedded Human Breast tissue by Immunohistochemistry.
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All lanes : Anti-Ezrin / Radixin / Moesin antibody (ab118572) at 1/500 dilution
Lane 1 : NIH-3T3 cell extract without the synthesized peptide.
Lane 2 : NIH-3T3 cell extract with the synthesized peptide.
Predicted band size: 68 kDa
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab118572 has been referenced in 1 publication.
- Guo C et al. Bilobalide reversibly modulates blood-brain barrier permeability through promoting adenosine A1 receptor-mediated phosphorylation of actin-binding proteins. Biochem Biophys Res Commun 526:1077-1084 (2020). PubMed: 32312522