Anti-F-actin antibody [NH3] (ab205)
- Datasheet
- References (56)
- Protocols
Overview
-
Product nameAnti-F-actin antibody [NH3]
See all F-actin primary antibodies -
DescriptionMouse monoclonal [NH3] to F-actin
-
Host speciesMouse
-
SpecificityClone NH3 recognises human Filamentous actin (F-actin) and reportedly also recognizes Globular actin (G-actin). The antibody binds to the N-terminal region of actin, but not to the extreme N-terminal 40 amino acids. In tissue sections the antibody stains the cytoplasm of macrophages strongly, and gives granular, localised nuclear staining of all cell types. As this is a mouse IgM antibody, an anti-mouse IgM secondary antibody must be used to detect this primary.
-
Tested applicationsSuitable for: Flow Cyt, ICC/IF, IHC-R, WB, ELISA, IHC-Fr, IPmore details
-
Species reactivityReacts with: Mouse, Rat, Rabbit, Human
-
Immunogen
Human monocytes and U397 cell line.
-
EpitopeThis antibody is reported to recognise actin in the filamentous form with the epitope likely to be located between residues 120 and 226 of the molecule.
-
Positive control
- gastrointestinal tissue
Properties
-
FormLiquid
-
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
-
Storage bufferPreservative: 0.1% Sodium azide
-
Concentration information loading... -
PurityPurified IgM
-
Purification notesPurified IgM prepared from tissue culture supernatant.
-
ClonalityMonoclonal
-
Clone numberNH3
-
MyelomaNS1
-
IsotypeIgM
-
Light chain typeunknown
-
Research areas
Associated products
-
Compatible Secondaries
-
Immunohistochemistry kits
-
Isotype control
-
Related Products
Applications
Our Abpromise guarantee covers the use of ab205 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt | 1/10. ab91545 - Mouse monoclonal IgM, is suitable for use as an isotype control with this antibody. |
|
| ICC/IF | 1/100. | |
| IHC-R | Use at an assay dependent concentration. | |
| WB | 1/100 - 1/500. Detects a band of approximately 43 kDa. As this is a mouse IgM antibody, an anti-mouse IgM secondary antibody must be used to detect this primary. | |
| ELISA | 1/10. | |
| IHC-Fr | Use at an assay dependent concentration. | |
| IP | Use at an assay dependent concentration. PubMed: 27336173 |
Target
-
FunctionActins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
-
Involvement in diseaseDefects in ACTB are a cause of dystonia juvenile-onset (DYTJ) [MIM:607371]. DYTJ is a form of dystonia with juvenile onset. Dystonia is defined by the presence of sustained involuntary muscle contraction, often leading to abnormal postures. DYTJ patients manifest progressive, generalized, dopa-unresponsive dystonia, developmental malformations and sensory hearing loss.
-
Sequence similaritiesBelongs to the actin family.
-
Post-translational
modificationsISGylated. -
Cellular localizationCytoplasm > cytoskeleton. Localized in cytoplasmic mRNP granules containing untranslated mRNAs.
- Information by UniProt
-
Database links
- Entrez Gene: 60 Human
- Entrez Gene: 11461 Mouse
- Entrez Gene: 100009272 Rabbit
- Entrez Gene: 81822 Rat
- Omim: 102630 Human
- SwissProt: P60709 Human
- SwissProt: P60710 Mouse
- SwissProt: P29751 Rabbit
see all -
Alternative names
- ACTB antibody
- ACTB_HUMAN antibody
- Actin, cytoplasmic 1, N-terminally processed antibody
see all
Images
-
![Western blot - Anti-F-actin antibody [NH3] (ab205)](https://www.abcam.com/ps/products/0/ab205/Images/ab205-3426-anti-f-actin-antibody-nh3-western-blot.jpg)
Western blot - Anti-F-actin antibody [NH3] (ab205)This image is courtesy of an anonymous AbreviewAnti-F-actin antibody [NH3] (ab205) at 1/100 dilution + Human pancreatic cancer cell line, whole cell lysate at 20 µg
Secondary
HRP conjugated goat anti-mouse antibody at 1/2000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Observed band size: 43 kDa why is the actual band size different from the predicted?
Exposure time: 1 minute
-
![Immunocytochemistry/ Immunofluorescence - Anti-F-actin antibody [NH3] (ab205)](https://www.abcam.com/ps/products/0/ab205/Images/ab205-274549-anti-f-actin-antibody-nh3-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-F-actin antibody [NH3] (ab205)Image from Ibrahim, Ahmed S. et al. Oncotarget 7.8 (2016): 8532–8545. doi: 10.18632/oncotarget.7384, Fig 6D.Immunocytochemistry/ Immunofluorescence analysis of ARPE cells labelling F-actin with ab205. Cells were fixed in 4% paraformaldehyde for 10 min, washed with PBS, and blocked for one hour. Cells were then incubated for 3h at 37°C with Anti-F-actin antibody [NH3] (ab205) at 1/200 dilution. Thereafter, cells were washed 3 times with PBS containing 0.3% Triton-X, incubated with secondary antibody and coverslipped with Fluoroshield containing DAPI as a counter stain. Immunofluorescent signals were detected by confocal microscopy.
-
![Immunohistochemistry (Resin sections) - Anti-F-actin antibody [NH3] (ab205)](https://www.abcam.com/ps/products/0/ab205/Images/ab205-274547-anti-f-actin-antibody-nh3-immunohistochemistry.jpg)
Immunohistochemistry (Resin sections) - Anti-F-actin antibody [NH3] (ab205)This image is courtesy of an anonymous abreview.Immunohistochemistry (Resin sections) analysis of mouse bone tissue cells labeling F-actin with ab205 at 1/200 dilution. Mouse tibia bone samples were fixed in neutral buffered formalin for 72 hours at room temperature, embedded undecalcified in plastic and sectioned at 5 microns. A polyclonal goat anti-mouse Alexa Fluor® 488 secondary antibody was used at 1/700 dilution. The image shows a 1 micron thick z-projection of the chondrocytes in the proximal tibia growth plate.
-
![Western blot - Anti-F-actin antibody [NH3] (ab205)](https://www.abcam.com/ps/products/0/ab205/Images/ab205-274545-anti-f-actin-antibody-nh3-western-blot.jpg)
Western blot - Anti-F-actin antibody [NH3] (ab205)This image is courtesy of an anonymous abreview.Anti-F-actin antibody [NH3] (ab205) at 1/500 dilution + CNS whole tissue lysate at 30 µg
Secondary
Goat anti-mouse HRP conjugate at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Observed band size: 43 kDa why is the actual band size different from the predicted?
Exposure time: 5 minutesBlocking: 5% milk for 1 hour at 25°C.
Incubation with primary antibody for 15 hours at 4°C in 5%milk+ PBS+0.1% Tween 20.
-
![Immunocytochemistry/ Immunofluorescence - Anti-F-actin antibody [NH3] (ab205)](https://www.abcam.com/ps/products/0/ab205/Images/ab205-274562-anti-f-actin-antibody-nh3-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-F-actin antibody [NH3] (ab205)This image is courtesy of an anonymous abreview.Immunocytochemistry/ Immunofluorescence analysis of mouse cortical neurons labeling F-actin with ab205 at 1/100 dilution. The cells were fixed with Ethanol and permeabilized with 0.2% Triton X-100. A polyclonal goat anti-mouse Cy3 conjugated secondary antibody was used at 1/200 dilution.
-
![Immunohistochemistry (Frozen sections) - Anti-F-actin antibody [NH3] (ab205)](https://www.abcam.com/ps/products/0/ab205/Images/ab205-3425-anti-f-actin-antibody-nh3-immunohistochemistry.jpg)
Immunohistochemistry (Frozen sections) - Anti-F-actin antibody [NH3] (ab205)Image courtesy of an Abreview submitted by Jamie Richards.ab205 staining F-actin in rat brain tissue by Immunohistochemistry (frozen sections). Tissue was fixed with paraformaldehyde and then blocked with 2% BSA for 1 hour followed by incubation with the primary antibody at a 1/25 dilution for 24 hours. A biotin-conjugated goat anti-mouse IgM was used as secondary antibody at a 1/400 dilution. No permeabilisation step was used.
Protocols
References
This product has been referenced in:
- Li H et al. Mst1 deletion attenuates renal ischaemia-reperfusion injury: The role of microtubule cytoskeleton dynamics, mitochondrial fission and the GSK3ß-p53 signalling pathway. Redox Biol 20:261-274 (2019). Read more (PubMed: 30384260) »
- Cheng B et al. MicroRNA-122 inhibits epithelial-mesenchymal transition of hepatic stellate cells induced by the TGF-ß1/Smad signaling pathway. Exp Ther Med 17:284-290 (2019). Read more (PubMed: 30651793) »
