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  1. Link

    f-actin-antibody-nh3-ab205.pdf

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Tags & Cell Markers Subcellular Markers Cytoskeleton Actin
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Anti-F-actin antibody [NH3] (ab205)

  • Datasheet
  • SDS
Reviews (11)Q&A (26)References (102)

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Key features and details

  • Mouse monoclonal [NH3] to F-actin
  • Suitable for: ELISA, IHC-Fr, ICC/IF, IHC-R, Flow Cyt, WB, IP
  • Reacts with: Mouse, Rat, Rabbit, Human
  • Isotype: IgM

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Overview

  • Product name

    Anti-F-actin antibody [NH3]
    See all F-actin primary antibodies
  • Description

    Mouse monoclonal [NH3] to F-actin
  • Host species

    Mouse
  • Specificity

    Clone NH3 recognises human Filamentous actin (F-actin) and reportedly also recognizes Globular actin (G-actin). The antibody binds to the N-terminal region of actin, but not to the extreme N-terminal 40 amino acids. In tissue sections the antibody stains the cytoplasm of macrophages strongly, and gives granular, localised nuclear staining of all cell types. As this is a mouse IgM antibody, an anti-mouse IgM secondary antibody must be used to detect this primary.
  • Tested applications

    Suitable for: ELISA, IHC-Fr, ICC/IF, IHC-R, Flow Cyt, WB, IPmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Rabbit, Human
  • Immunogen

    Tissue, cells or virus corresponding to F-actin. Human monocytes and U397 cell line.

  • Epitope

    This antibody is reported to recognise actin in the filamentous form with the epitope likely to be located between residues 120 and 226 of the molecule.
  • Positive control

    • gastrointestinal tissue
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.1% Sodium azide
  • Concentration information loading...
  • Purity

    Purified IgM
  • Purification notes

    Purified IgM prepared from tissue culture supernatant.
  • Clonality

    Monoclonal
  • Clone number

    NH3
  • Myeloma

    NS1
  • Isotype

    IgM
  • Light chain type

    unknown
  • Research areas

    • Tags & Cell Markers
    • Subcellular Markers
    • Cytoskeleton
    • Actin
    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Microfilaments
    • Actin etc
    • Actin

Associated products

  • Compatible Secondaries

    • Rabbit Anti-Mouse IgG+IgM+IgA H&L (ab8516)
    • Rabbit Anti-Mouse IgG+IgM+IgA H&L (FITC) (ab8517)
    • Goat Anti-Mouse IgM H&L (ab9167)
    • Rabbit Anti-Mouse IgM mu chain (ab9175)
  • Isotype control

    • Mouse IgM [B11/7] - Isotype control (ab91545)
  • Related Products

    • F-actin Staining Kit - Red Fluorescence - Cytopainter (ab112127)
    • Phalloidin-iFluor 488 Reagent (ab176753)
    • Phalloidin-iFluor 647 Reagent (ab176759)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab205 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA
Use at an assay dependent concentration.
IHC-Fr (2)
Use at an assay dependent concentration.
ICC/IF (3)
Use at an assay dependent concentration.
IHC-R (1)
Use at an assay dependent concentration.
Flow Cyt
Use at an assay dependent concentration.

ab91545 - Mouse monoclonal IgM, is suitable for use as an isotype control with this antibody.

WB (4)
Use at an assay dependent concentration. Predicted molecular weight: 41 kDa.

As this is a mouse IgM antibody, an anti-mouse IgM secondary antibody must be used to detect this primary.

IP
Use at an assay dependent concentration. PubMed: 27336173
Notes
ELISA
Use at an assay dependent concentration.
IHC-Fr
Use at an assay dependent concentration.
ICC/IF
Use at an assay dependent concentration.
IHC-R
Use at an assay dependent concentration.
Flow Cyt
Use at an assay dependent concentration.

ab91545 - Mouse monoclonal IgM, is suitable for use as an isotype control with this antibody.

WB
Use at an assay dependent concentration. Predicted molecular weight: 41 kDa.

As this is a mouse IgM antibody, an anti-mouse IgM secondary antibody must be used to detect this primary.

IP
Use at an assay dependent concentration. PubMed: 27336173

Target

  • Function

    Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
  • Involvement in disease

    Defects in ACTB are a cause of dystonia juvenile-onset (DYTJ) [MIM:607371]. DYTJ is a form of dystonia with juvenile onset. Dystonia is defined by the presence of sustained involuntary muscle contraction, often leading to abnormal postures. DYTJ patients manifest progressive, generalized, dopa-unresponsive dystonia, developmental malformations and sensory hearing loss.
  • Sequence similarities

    Belongs to the actin family.
  • Post-translational
    modifications

    ISGylated.
  • Cellular localization

    Cytoplasm > cytoskeleton. Localized in cytoplasmic mRNP granules containing untranslated mRNAs.
  • Target information above from: UniProt accession P60709 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 60 Human
    • Entrez Gene: 11461 Mouse
    • Entrez Gene: 100009272 Rabbit
    • Entrez Gene: 81822 Rat
    • Omim: 102630 Human
    • SwissProt: P60709 Human
    • SwissProt: P60710 Mouse
    • SwissProt: P29751 Rabbit
    • SwissProt: P60711 Rat
    • Unigene: 520640 Human
    • Unigene: 708120 Human
    • Unigene: 727576 Human
    • Unigene: 328431 Mouse
    • Unigene: 391967 Mouse
    • Unigene: 94978 Rat
    see all
  • Alternative names

    • ACTB antibody
    • ACTB_HUMAN antibody
    • Actin, cytoplasmic 1, N-terminally processed antibody
    • Beta-actin antibody
    • f actin antibody
    • Filamentous actin antibody
    see all

Protocols

  • Immunohistochemistry protocols
  • Immunocytochemistry & immunofluorescence protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (102)

Publishing research using ab205? Please let us know so that we can cite the reference in this datasheet.

ab205 has been referenced in 102 publications.

  • Liu WL  et al. High Mobility Group Box 1 Promotes Lung Cancer Cell Migration and Motility via Regulation of Dynamin-Related Protein 1. Int J Mol Sci 22:N/A (2021). PubMed: 33807275
  • Zhong Y  et al. Inhibiting Th1/2 cells influences hepatic capillarization by adjusting sinusoidal endothelial fenestrae through Rho-ROCK-myosin pathway. Aging (Albany NY) 13:5069-5086 (2021). PubMed: 33535174
  • Kong M  et al. Myocardin-related transcription factor A nuclear translocation contributes to mechanical overload-induced nucleus pulposus fibrosis in rats with intervertebral disc degeneration. Int J Mol Med 48:N/A (2021). PubMed: 33982787
  • Hattori K  et al. The Infantile Leukoencephalopathy-Associated Mutation of C11ORF73/HIKESHI Proteins Generates de novo Interactive Activity with Filamin A, Inhibiting Oligodendroglial Cell Morphological Differentiation. Medicines (Basel) 8:N/A (2021). PubMed: 33535532
  • Liu Y  et al. Cell Softness Prevents Cytolytic T-cell Killing of Tumor-Repopulating Cells. Cancer Res 81:476-488 (2021). PubMed: 33168645
View all Publications for this product

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

1-10 of 37 Abreviews or Q&A

Immunocytochemistry abreview for Anti-F-actin antibody [NH3]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry
Sample
Mouse Cell (mouse corneal cells)
Permeabilization
Yes - 1%BSA + 1% Triton X-100
Specification
mouse corneal cells
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Paraformaldehyde
Read More

DR. Daan Viering

Verified customer

Submitted Jan 03 2022

Immunocytochemistry/ Immunofluorescence abreview for Anti-F-actin antibody [NH3]

Average
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (U2OS)
Permeabilization
Yes - NP40
Specification
U2OS
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 21°C
Fixative
Formaldehyde
Read More

Abcam user community

Verified customer

Submitted May 29 2019

Western blot abreview for Anti-F-actin antibody [NH3]

Poor
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (U2OS)
Gel Running Conditions
Reduced Denaturing
Loading amount
20 µg
Specification
U2OS
Blocking step
Milk as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Read More

Abcam user community

Verified customer

Submitted Apr 05 2019

Immunohistochemistry (Frozen sections) abreview for Anti-F-actin antibody [NH3]

Good
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue (Pancreas PanIN)
Permeabilization
No
Specification
Pancreas PanIN
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C
Fixative
Methanol
Read More

Abcam user community

Verified customer

Submitted Jun 27 2018

Immunocytochemistry/ Immunofluorescence abreview for Anti-F-actin antibody [NH3]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (Mouse Derived Breast cancer Cell Line)
Permeabilization
Yes - 0.5 % Triton
Specification
Mouse Derived Breast cancer Cell Line
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 26°C
Fixative
Methanol
Read More

Abcam user community

Verified customer

Submitted Aug 17 2017

Western blot abreview for Anti-F-actin antibody [NH3]

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Mouse Cell lysate - whole cell (Mouse Derived Breast Cancer Primary Cell Line)
Gel Running Conditions
Reduced Denaturing (10)
Loading amount
40 µg
Specification
Mouse Derived Breast Cancer Primary Cell Line
Blocking step
Licor Blocking Buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 26°C
Read More

Abcam user community

Verified customer

Submitted Aug 14 2017

Immunocytochemistry/ Immunofluorescence abreview for Anti-F-actin antibody [NH3]

Good
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (Cortical Neurons)
Permeabilization
Yes - 0.2% Triton X-100
Specification
Cortical Neurons
Blocking step
casein solution + Tween 20 as blocking agent for 20 minute(s) · Concentration: 0.2% · Temperature: 25°C
Fixative
Ethanol
Read More

Abcam user community

Verified customer

Submitted Aug 04 2016

Western blot abreview for Anti-F-actin antibody [NH3]

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Loading amount
30 µg
Gel Running Conditions
Reduced Denaturing (12)
Sample
Human Tissue lysate - whole (CNS)
Specification
CNS
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More

Abcam user community

Verified customer

Submitted Aug 01 2014

Immunohistochemistry (Resin sections) abreview for Anti-F-actin antibody [NH3]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Resin sections)
Sample
Mouse Tissue sections (Bone Tissue Cells)
Specification
Bone Tissue Cells
Read More

Abcam user community

Verified customer

Submitted Jul 10 2013

Question

Je suis à la recherche d’un anticorps spécifique de l’actine F et un autre spécifique de l’actine monomérique ou actin-G.

J’aimerais un anticorps déjà testé en Immunofluorescence et pas de toxine type phalloidin.

Read More

Abcam community

Verified customer

Asked on Sep 01 2014

Answer

Je peux vous proposer ab205, Anti-F-actin antibody [NH3] qui est un monoclonal de souris garanti de fonctionner en immunofluorescence. Cependant, nous n’avons pas testé le taux de toxine dans ce produit et donc nous ne pouvons pas garantir qu’il n’y a pas de toxine type phalloidin présent.


Malheureusement, nous n’avons pas d’anticorps anti-G-actine. Tous nos autres anticorps reconnaissent les deux formes F et G.

Read More

Elisa Thomas

Abcam Scientific Support

Answered on Sep 01 2014

1-10 of 37 Abreviews or Q&A

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