Anti-F-actin antibody [NH3] (ab205)
Key features and details
- Mouse monoclonal [NH3] to F-actin
- Reacts with: Rat, Rabbit, Human
- Isotype: IgM
Overview
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Product name
Anti-F-actin antibody [NH3]
See all F-actin primary antibodies -
Description
Mouse monoclonal [NH3] to F-actin -
Host species
Mouse -
Specificity
Clone NH3 recognises human Filamentous actin (F-actin) and reportedly also recognizes Globular actin (G-actin). The antibody binds to the N-terminal region of actin, but not to the extreme N-terminal 40 amino acids. In tissue sections the antibody stains the cytoplasm of macrophages strongly, and gives granular, localised nuclear staining of all cell types. As this is a mouse IgM antibody, an anti-mouse IgM secondary antibody must be used to detect this primary. -
Species reactivity
Reacts with: Rat, Rabbit, Human -
Immunogen
Human monocytes and U397 cell line.
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Epitope
This antibody is reported to recognise actin in the filamentous form with the epitope likely to be located between residues 120 and 226 of the molecule. -
Positive control
- gastrointestinal tissue
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.1% Sodium azide -
Concentration information loading... -
Purity
Purified IgM -
Purification notes
Purified IgM prepared from tissue culture supernatant. -
Clonality
Monoclonal -
Clone number
NH3 -
Myeloma
NS1 -
Isotype
IgM -
Light chain type
unknown -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Related Products
Target
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Function
Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. -
Involvement in disease
Defects in ACTB are a cause of dystonia juvenile-onset (DYTJ) [MIM:607371]. DYTJ is a form of dystonia with juvenile onset. Dystonia is defined by the presence of sustained involuntary muscle contraction, often leading to abnormal postures. DYTJ patients manifest progressive, generalized, dopa-unresponsive dystonia, developmental malformations and sensory hearing loss. -
Sequence similarities
Belongs to the actin family. -
Post-translational
modificationsISGylated. -
Cellular localization
Cytoplasm > cytoskeleton. Localized in cytoplasmic mRNP granules containing untranslated mRNAs. - Information by UniProt
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Database links
- Entrez Gene: 60 Human
- Entrez Gene: 100009272 Rabbit
- Entrez Gene: 81822 Rat
- Omim: 102630 Human
- SwissProt: P60709 Human
- SwissProt: P29751 Rabbit
- SwissProt: P60711 Rat
- Unigene: 520640 Human
see all -
Alternative names
- ACTB antibody
- ACTB_HUMAN antibody
- Actin, cytoplasmic 1, N-terminally processed antibody
see all
Protocols
References (79)
ab205 has been referenced in 79 publications.
- Zhang Q et al. Long noncoding RNA MAGI1-IT1 regulates cardiac hypertrophy by modulating miR-302e/DKK1/Wnt/beta-catenin signaling pathway. J Cell Physiol 235:245-253 (2020). PubMed: 31222747
- Zheng Z et al. NR4A1 promotes TNF-a-induced chondrocyte death and migration injury via activating the AMPK/Drp1/mitochondrial fission pathway. Int J Mol Med 45:151-161 (2020). PubMed: 31746366
- Zhao Y et al. AQP1 suppression by ATF4 triggers trabecular meshwork tissue remodelling in ET-1-induced POAG. J Cell Mol Med 24:3469-3480 (2020). PubMed: 32052937
- Zhang X et al. miR-31 Modulates Liver Cancer HepG2 Cell Apoptosis and Invasion via ROCK1/F-Actin Pathways. Onco Targets Ther 13:877-888 (2020). PubMed: 32099392
- Ye D et al. Short-wave enhances mesenchymal stem cell recruitment in fracture healing by increasing HIF-1 in callus. Stem Cell Res Ther 11:382 (2020). PubMed: 32894200
