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    f-actin-antibody-nh3-ab205.pdf

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Tags & Cell Markers Subcellular Markers Cytoskeleton Actin
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Anti-F-actin antibody [NH3] (ab205)

  • Datasheet
  • SDS
Reviews (10)Q&A (26)References (79)

Product price, shipping and contact information

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Key features and details

  • Mouse monoclonal [NH3] to F-actin
  • Reacts with: Rat, Rabbit, Human
  • Isotype: IgM

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Overview

  • Product name

    Anti-F-actin antibody [NH3]
    See all F-actin primary antibodies
  • Description

    Mouse monoclonal [NH3] to F-actin
  • Host species

    Mouse
  • Specificity

    Clone NH3 recognises human Filamentous actin (F-actin) and reportedly also recognizes Globular actin (G-actin). The antibody binds to the N-terminal region of actin, but not to the extreme N-terminal 40 amino acids. In tissue sections the antibody stains the cytoplasm of macrophages strongly, and gives granular, localised nuclear staining of all cell types. As this is a mouse IgM antibody, an anti-mouse IgM secondary antibody must be used to detect this primary.
  • Species reactivity

    Reacts with: Rat, Rabbit, Human
  • Immunogen

    Human monocytes and U397 cell line.

  • Epitope

    This antibody is reported to recognise actin in the filamentous form with the epitope likely to be located between residues 120 and 226 of the molecule.
  • Positive control

    • gastrointestinal tissue

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.1% Sodium azide
  • Concentration information loading...
  • Purity

    Purified IgM
  • Purification notes

    Purified IgM prepared from tissue culture supernatant.
  • Clonality

    Monoclonal
  • Clone number

    NH3
  • Myeloma

    NS1
  • Isotype

    IgM
  • Light chain type

    unknown
  • Research areas

    • Tags & Cell Markers
    • Subcellular Markers
    • Cytoskeleton
    • Actin
    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Microfilaments
    • Actin etc
    • Actin

Associated products

  • Compatible Secondaries

    • Rabbit Anti-Mouse IgG+IgM+IgA H&L (ab8516)
    • Rabbit Anti-Mouse IgG+IgM+IgA H&L (FITC) (ab8517)
    • Goat Anti-Mouse IgM H&L (ab9167)
    • Rabbit Anti-Mouse IgM mu chain (ab9175)
  • Isotype control

    • Mouse IgM [B11/7] - Isotype control (ab91545)
  • Related Products

    • F-actin Staining Kit - Red Fluorescence - Cytopainter (ab112127)
    • Phalloidin-iFluor 488 Reagent (ab176753)
    • Phalloidin-iFluor 647 Reagent (ab176759)

Target

  • Function

    Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
  • Involvement in disease

    Defects in ACTB are a cause of dystonia juvenile-onset (DYTJ) [MIM:607371]. DYTJ is a form of dystonia with juvenile onset. Dystonia is defined by the presence of sustained involuntary muscle contraction, often leading to abnormal postures. DYTJ patients manifest progressive, generalized, dopa-unresponsive dystonia, developmental malformations and sensory hearing loss.
  • Sequence similarities

    Belongs to the actin family.
  • Post-translational
    modifications

    ISGylated.
  • Cellular localization

    Cytoplasm > cytoskeleton. Localized in cytoplasmic mRNP granules containing untranslated mRNAs.
  • Target information above from: UniProt accession P60709 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 60 Human
    • Entrez Gene: 100009272 Rabbit
    • Entrez Gene: 81822 Rat
    • Omim: 102630 Human
    • SwissProt: P60709 Human
    • SwissProt: P29751 Rabbit
    • SwissProt: P60711 Rat
    • Unigene: 520640 Human
    • Unigene: 708120 Human
    • Unigene: 727576 Human
    • Unigene: 94978 Rat
    see all
  • Alternative names

    • ACTB antibody
    • ACTB_HUMAN antibody
    • Actin, cytoplasmic 1, N-terminally processed antibody
    • Beta-actin antibody
    • f actin antibody
    • Filamentous actin antibody
    see all

Protocols

  • Immunohistochemistry protocols
  • Immunocytochemistry & immunofluorescence protocols

Click here to view the general protocols

Datasheets and documents

    • Datasheet
    • SDS
  • References (79)

    Publishing research using ab205? Please let us know so that we can cite the reference in this datasheet.

    ab205 has been referenced in 79 publications.

    • Zhang Q  et al. Long noncoding RNA MAGI1-IT1 regulates cardiac hypertrophy by modulating miR-302e/DKK1/Wnt/beta-catenin signaling pathway. J Cell Physiol 235:245-253 (2020). PubMed: 31222747
    • Zheng Z  et al. NR4A1 promotes TNF-a-induced chondrocyte death and migration injury via activating the AMPK/Drp1/mitochondrial fission pathway. Int J Mol Med 45:151-161 (2020). PubMed: 31746366
    • Zhao Y  et al. AQP1 suppression by ATF4 triggers trabecular meshwork tissue remodelling in ET-1-induced POAG. J Cell Mol Med 24:3469-3480 (2020). PubMed: 32052937
    • Zhang X  et al. miR-31 Modulates Liver Cancer HepG2 Cell Apoptosis and Invasion via ROCK1/F-Actin Pathways. Onco Targets Ther 13:877-888 (2020). PubMed: 32099392
    • Ye D  et al. Short-wave enhances mesenchymal stem cell recruitment in fracture healing by increasing HIF-1 in callus. Stem Cell Res Ther 11:382 (2020). PubMed: 32894200
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-10 of 36 Abreviews or Q&A

    Immunocytochemistry/ Immunofluorescence abreview for Anti-F-actin antibody [NH3]

    Average
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (U2OS)
    Permeabilization
    Yes - NP40
    Specification
    U2OS
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 21°C
    Fixative
    Formaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted May 29 2019

    Western blot abreview for Anti-F-actin antibody [NH3]

    Poor
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (U2OS)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    20 µg
    Specification
    U2OS
    Blocking step
    Milk as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
    Read More

    Abcam user community

    Verified customer

    Submitted Apr 05 2019

    Immunohistochemistry (Frozen sections) abreview for Anti-F-actin antibody [NH3]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue (Pancreas PanIN)
    Permeabilization
    No
    Specification
    Pancreas PanIN
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C
    Fixative
    Methanol
    Read More

    Abcam user community

    Verified customer

    Submitted Jun 27 2018

    Immunocytochemistry/ Immunofluorescence abreview for Anti-F-actin antibody [NH3]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Mouse Cell (Mouse Derived Breast cancer Cell Line)
    Permeabilization
    Yes - 0.5 % Triton
    Specification
    Mouse Derived Breast cancer Cell Line
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 26°C
    Fixative
    Methanol
    Read More

    Abcam user community

    Verified customer

    Submitted Aug 17 2017

    Western blot abreview for Anti-F-actin antibody [NH3]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (Mouse Derived Breast Cancer Primary Cell Line)
    Gel Running Conditions
    Reduced Denaturing (10)
    Loading amount
    40 µg
    Specification
    Mouse Derived Breast Cancer Primary Cell Line
    Blocking step
    Licor Blocking Buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 26°C
    Read More

    Abcam user community

    Verified customer

    Submitted Aug 14 2017

    Immunocytochemistry/ Immunofluorescence abreview for Anti-F-actin antibody [NH3]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Mouse Cell (Cortical Neurons)
    Permeabilization
    Yes - 0.2% Triton X-100
    Specification
    Cortical Neurons
    Blocking step
    casein solution + Tween 20 as blocking agent for 20 minute(s) · Concentration: 0.2% · Temperature: 25°C
    Fixative
    Ethanol
    Read More

    Abcam user community

    Verified customer

    Submitted Aug 04 2016

    Question

    Je suis à la recherche d’un anticorps spécifique de l’actine F et un autre spécifique de l’actine monomérique ou actin-G.

    J’aimerais un anticorps déjà testé en Immunofluorescence et pas de toxine type phalloidin.

    Read More

    Abcam community

    Verified customer

    Asked on Sep 01 2014

    Answer

    Je peux vous proposer ab205, Anti-F-actin antibody [NH3] qui est un monoclonal de souris garanti de fonctionner en immunofluorescence. Cependant, nous n’avons pas testé le taux de toxine dans ce produit et donc nous ne pouvons pas garantir qu’il n’y a pas de toxine type phalloidin présent.


    Malheureusement, nous n’avons pas d’anticorps anti-G-actine. Tous nos autres anticorps reconnaissent les deux formes F et G.

    Read More

    Elisa Thomas

    Abcam Scientific Support

    Answered on Sep 01 2014

    Western blot abreview for Anti-F-actin antibody [NH3]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Loading amount
    30 µg
    Gel Running Conditions
    Reduced Denaturing (12)
    Sample
    Human Tissue lysate - whole (CNS)
    Specification
    CNS
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    Submitted Aug 01 2014

    Immunohistochemistry (Resin sections) abreview for Anti-F-actin antibody [NH3]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Resin sections)
    Sample
    Mouse Tissue sections (Bone Tissue Cells)
    Specification
    Bone Tissue Cells
    Read More

    Abcam user community

    Verified customer

    Submitted Jul 10 2013

    Question

    I got the reply from the customer in regards to ab205:
    "I have tried, according to your suggestion, to do the IHC experiment again with a different secondary antibody "Alexa Fluor® 488 Goat Anti-Mouse IgG, IgM (H+L)" that recognize both IgG and IgM. This didn't recognize the F-actin antibody and resulted in no positive-specific staining.
    At this point I think I have tried almost every step in the attempts to get a positive signal."

    Looking forward to your reply.
    Thanks in advance for your assistance.

    Best Regards,

    Read More

    Abcam community

    Verified customer

    Asked on Dec 04 2012

    Answer

    Thank you for your message and for providing this further information.
    I am sorry to hear the suggestions made have not improved the results on this occasion. I appreciate the time you have spent on these experiments and would be pleased to arrange a free of charge replacement or refund in compensation.
    If you decide to get a free of charge replacement we would recommend it would be beneficial to include a positive control, such as gastrointestinal tissue or brain tissue in the following experiments.
    I look forward hearing from you with details of how you would like to proceed.

    Read More

    Abcam Scientific Support

    Answered on Dec 04 2012

    1-10 of 36 Abreviews or Q&A

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