Anti-F-actin antibody [NH3] (ab205)
Key features and details
- Mouse monoclonal [NH3] to F-actin
- Suitable for: ELISA, IHC-Fr, ICC/IF, IHC-R, Flow Cyt, WB, IP
- Reacts with: Mouse, Rat, Rabbit, Human
- Isotype: IgM
Overview
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Product name
Anti-F-actin antibody [NH3]
See all F-actin primary antibodies -
Description
Mouse monoclonal [NH3] to F-actin -
Host species
Mouse -
Specificity
Clone NH3 recognises human Filamentous actin (F-actin) and reportedly also recognizes Globular actin (G-actin). The antibody binds to the N-terminal region of actin, but not to the extreme N-terminal 40 amino acids. In tissue sections the antibody stains the cytoplasm of macrophages strongly, and gives granular, localised nuclear staining of all cell types. As this is a mouse IgM antibody, an anti-mouse IgM secondary antibody must be used to detect this primary. -
Tested applications
Suitable for: ELISA, IHC-Fr, ICC/IF, IHC-R, Flow Cyt, WB, IPmore details -
Species reactivity
Reacts with: Mouse, Rat, Rabbit, Human -
Immunogen
Tissue, cells or virus corresponding to F-actin. Human monocytes and U397 cell line.
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Epitope
This antibody is reported to recognise actin in the filamentous form with the epitope likely to be located between residues 120 and 226 of the molecule. -
Positive control
- gastrointestinal tissue
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.1% Sodium azide -
Concentration information loading... -
Purity
Purified IgM -
Purification notes
Purified IgM prepared from tissue culture supernatant. -
Clonality
Monoclonal -
Clone number
NH3 -
Myeloma
NS1 -
Isotype
IgM -
Light chain type
unknown -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab205 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ELISA |
Use at an assay dependent concentration.
|
|
| IHC-Fr | (2) |
Use at an assay dependent concentration.
|
| ICC/IF | (3) |
Use at an assay dependent concentration.
|
| IHC-R | (1) |
Use at an assay dependent concentration.
|
| Flow Cyt |
Use at an assay dependent concentration.
ab91545 - Mouse monoclonal IgM, is suitable for use as an isotype control with this antibody. |
|
| WB | (4) |
Use at an assay dependent concentration. Predicted molecular weight: 41 kDa.
As this is a mouse IgM antibody, an anti-mouse IgM secondary antibody must be used to detect this primary. |
| IP |
Use at an assay dependent concentration. PubMed: 27336173
|
| Notes |
|---|
|
ELISA
Use at an assay dependent concentration. |
|
IHC-Fr
Use at an assay dependent concentration. |
|
ICC/IF
Use at an assay dependent concentration. |
|
IHC-R
Use at an assay dependent concentration. |
|
Flow Cyt
Use at an assay dependent concentration. ab91545 - Mouse monoclonal IgM, is suitable for use as an isotype control with this antibody. |
|
WB
Use at an assay dependent concentration. Predicted molecular weight: 41 kDa. As this is a mouse IgM antibody, an anti-mouse IgM secondary antibody must be used to detect this primary. |
|
IP
Use at an assay dependent concentration. PubMed: 27336173 |
Target
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Function
Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. -
Involvement in disease
Defects in ACTB are a cause of dystonia juvenile-onset (DYTJ) [MIM:607371]. DYTJ is a form of dystonia with juvenile onset. Dystonia is defined by the presence of sustained involuntary muscle contraction, often leading to abnormal postures. DYTJ patients manifest progressive, generalized, dopa-unresponsive dystonia, developmental malformations and sensory hearing loss. -
Sequence similarities
Belongs to the actin family. -
Post-translational
modificationsISGylated. -
Cellular localization
Cytoplasm > cytoskeleton. Localized in cytoplasmic mRNP granules containing untranslated mRNAs. - Information by UniProt
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Database links
- Entrez Gene: 60 Human
- Entrez Gene: 11461 Mouse
- Entrez Gene: 100009272 Rabbit
- Entrez Gene: 81822 Rat
- Omim: 102630 Human
- SwissProt: P60709 Human
- SwissProt: P60710 Mouse
- SwissProt: P29751 Rabbit
see all -
Alternative names
- ACTB antibody
- ACTB_HUMAN antibody
- Actin, cytoplasmic 1, N-terminally processed antibody
see all
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (102)
ab205 has been referenced in 102 publications.
- Liu WL et al. High Mobility Group Box 1 Promotes Lung Cancer Cell Migration and Motility via Regulation of Dynamin-Related Protein 1. Int J Mol Sci 22:N/A (2021). PubMed: 33807275
- Zhong Y et al. Inhibiting Th1/2 cells influences hepatic capillarization by adjusting sinusoidal endothelial fenestrae through Rho-ROCK-myosin pathway. Aging (Albany NY) 13:5069-5086 (2021). PubMed: 33535174
- Kong M et al. Myocardin-related transcription factor A nuclear translocation contributes to mechanical overload-induced nucleus pulposus fibrosis in rats with intervertebral disc degeneration. Int J Mol Med 48:N/A (2021). PubMed: 33982787
- Hattori K et al. The Infantile Leukoencephalopathy-Associated Mutation of C11ORF73/HIKESHI Proteins Generates de novo Interactive Activity with Filamin A, Inhibiting Oligodendroglial Cell Morphological Differentiation. Medicines (Basel) 8:N/A (2021). PubMed: 33535532
- Liu Y et al. Cell Softness Prevents Cytolytic T-cell Killing of Tumor-Repopulating Cells. Cancer Res 81:476-488 (2021). PubMed: 33168645