F-actin Staining Kit - Blue Fluorescence - Cytopainter (ab112124)

Overview

  • Product name
    F-actin Staining Kit - Blue Fluorescence - Cytopainter
    See all F-actin kits
  • Sample type
    Tissue, Adherent cells, Suspension cells
  • Assay type
    Cell-based (qualitative)
  • Species reactivity
    Reacts with: Other species, Mammals
  • Product overview

    F-actin Staining Kit - Blue Fluorescence | Cytopainter (ab112124) fluorescence imaging kits are a set of fluorescence imaging tools for labeling sub-cellular organelles such as lysosomes, mitochondria, and actin filaments. The selective staining of cell compartments provides a powerful method for studying cellular events in a spatial and temporal context.


    ab112124 is designed to stain F-actins in fixed cells with blue fluorescence. The kit uses a blue fluorescent phalloidin conjugate that is selectively bound to F-actins. The phalloidin conjugate has Ex/Em = 350/450 nm, compatible with DAPI filter set that comes with most of fluorescence microscopes. It is a high-affinity probe for labeling, identifying and quantitating F-actins in formaldehyde-fixed and permeabilized tissue sections, cell cultures or cell-free experiments. ab112124 provides all the essential components with an optimized labeling protocol.


     

  • Notes

    Store ab112124 desiccated.

  • Tested applications
    Suitable for: FM, ICC/IFmore details

Properties

Applications

Our Abpromise guarantee covers the use of ab112124 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
FM Use at an assay dependent concentration.

Use formaldehyde-fixed and permeabilized tissue sections or cell culture.

ICC/IF Use at an assay dependent concentration.

Use formaldehyde-fixed and permeabilized tissue sections or cell culture.

Images

  • Image: Courtesy of Dr. Shaohua Li, UMDNJ-Robert Wood Johnson Medical School

    Sample: mouse embryonic stem cell-differentiated embryoid bodies (EBs)

    Preparation:

    • Fix in 3%PFA in PBS for 30 min at RT
    • Incubate in 7.5% sucrose-PBS for 3h at RT
    • Incubate in 15% sucrose-PBS at 4 degree Celsius overnight
    • Embed the EBs in tissue-Tek OCT compound
    • Cut frozen sections to 4-20 µm thickness

    Primary antibody: Rabbit anti laminin alpha 1, 1:400
    Secondary antibody: Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Cy5®) (ab97077), 1:100
    F-actin was stained with CytoPainter blue (ab112124), 1:100
    Nuclei were counterstained with SYTO 16

  • CytoPainter F-actin Labelling Kit - Blue Fluorescence (ab112124) - CPA cells were fixed with 4.0% formaldehyde in a Costar black 96-well plate.
    Left panel: Cells were labelled with CytoPainter F-actin Labelling Kit - Blue for 30 minutes. Right panel: Cells were pre-treated  for 10 minutes with phalloidin, prior staining with CytoPainter F-actin Labelling Kit - Blue for 30 minutes.

Protocols

References

ab112124 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Thank you for contacting us.

The CytoPainter kits are compatible with secondary antibodies and nuclear dyes for multicolor staining or GFP-transfected cells, for example. The lab has confirmed that these kits can be used with other antibodies...

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Thank you for contacting us. To perform IHC-P with fluorescent secondary antibodies, I would recommend the following protocol:

De-paraffinise sections thoroughly in Xylene/synthetic solvent and hydrate through graded series of alcohols. Wash ...

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Thank you for your phone call. The dyes we were discussing are available in several colors, and the full list can be found here:

https://www.abcam.com/CytoPainter


I hope this helps, please let me know if you have any other ques...

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Thank you for your enquiry. I have looked into this issue and ab112124 does only stain dead (fixed) cells. Sorry for the confusion. Please feel free to contact me with any further questions.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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