F-actin Staining Kit - Green Fluorescence - Cytopainter (ab112125)
Key features and details
- Assay type: Cell-based (qualitative)
- Platform: Fluorescence microscope
- Sample type: Adherent cells, Suspension cells, Tissue
Overview
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Product name
F-actin Staining Kit - Green Fluorescence - Cytopainter
See all F-actin kits -
Sample type
Tissue, Adherent cells, Suspension cells -
Assay type
Cell-based (qualitative) -
Product overview
F-actin Staining Kit - Green Fluorescence | Cytopainter (ab112125) fluorescence imaging kits are a set of fluorescence imaging tools for labeling sub-cellular organelles such as lysosomes, mitochondria, and actin filaments. The selective staining of cell compartments provides a powerful method for studying cellular events in a spatial and temporal context.
ab112125 is designed to stain F-actins in fixed cells with green fluorescence. The kit uses a green fluorescent phalloidin conjugate that is selectively bound to F-actins. This green fluorescent phalloidin conjugate is a high-affinity probe for F-actins. When used at nanomolar concentrations, phallotoxins are convenient probes for labeling, identifying and quantitating F-actins in formaldehyde-fixed and permeabilized tissue sections, cell cultures or cell-free experiments.
ab112125 provides all the essential components with an optimized staining protocol, which is robust requiring minimal hands-on time. It is an excellent tool for preserving the fluorescent images of particular cells. The phalloidin conjugate has spectral properties similar to those of FITC (Ex/Em = 500/520 nm). It can also be used for fluorescence microscope demonstrations using FITC filter (Ex/Em = 490/525 nm).
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Notes
ab112125 should be stored dessicated.
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Platform
Fluorescence microscope
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 500 tests Green Fluorescent Phalloidin Conjugate 1 x 50µl Labeling Buffer 1 x 50ml -
Research areas
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Alternative names
- actin filament
- f actin
- Filamentous actin
Images
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Left Panel: Cells labeled with 1X Green Fluorescent Phalloidin Conjugate for 30 minutes only.
Right Panel: Cells treated with phalloidin for 10 minutes, then stained with Green Fluorescent Phalloidin Conjugate for 30 minutes.
Datasheets and documents
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SDS download
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Datasheet download
References (5)
ab112125 has been referenced in 5 publications.
- Lou X et al. VIP Stabilizes the Cytoskeleton of Schlemm's Canal Endothelia via Reducing Caspase-3 Mediated ZO-1 Endolysosomal Degradation. Oxid Med Cell Longev 2021:9397960 (2021). PubMed: 34552687
- Dashtimoghadam E et al. Microfluidic fabrication of microcarriers with sequential delivery of VEGF and BMP-2 for bone regeneration. Sci Rep 10:11764 (2020). PubMed: 32678204
- Song Y et al. Epithelial C5aR1 Signaling Enhances Uropathogenic Escherichia coli Adhesion to Human Renal Tubular Epithelial Cells. Front Immunol 9:949 (2018). PubMed: 29765378
- Gong X et al. Expression of ILK in renal stroma is essential for multiple aspects of renal development. Am J Physiol Renal Physiol 315:F374-F385 (2018). PubMed: 29638158
- Banni M et al. Assessing the impact of Benzo[a]pyrene on Marine Mussels: Application of a novel targeted low density microarray complementing classical biomarker responses. PLoS One 12:e0178460 (2017). PubMed: 28651000