Overview

  • Product name
    F-actin Staining Kit - Green Fluorescence - Cytopainter
    See all F-actin kits
  • Sample type
    Tissue, Adherent cells, Suspension cells
  • Assay type
    Cell-based (qualitative)
  • Product overview

    F-actin Staining Kit - Green Fluorescence | Cytopainter (ab112125) fluorescence imaging kits are a set of fluorescence imaging tools for labeling sub-cellular organelles such as lysosomes, mitochondria, and actin filaments. The selective staining of cell compartments provides a powerful method for studying cellular events in a spatial and temporal context.


    ab112125 is designed to stain F-actins in fixed cells with green fluorescence. The kit uses a green fluorescent phalloidin conjugate that is selectively bound to F-actins. This green fluorescent phalloidin conjugate is a high-affinity probe for F-actins. When used at nanomolar concentrations, phallotoxins are convenient probes for labeling, identifying and quantitating F-actins in formaldehyde-fixed and permeabilized tissue sections, cell cultures or cell-free experiments.


    ab112125 provides all the essential components with an optimized staining protocol, which is robust requiring minimal hands-on time. It is an excellent tool for preserving the fluorescent images of particular cells. The phalloidin conjugate has spectral properties similar to those of FITC (Ex/Em = 500/520 nm). It can also be used for fluorescence microscope demonstrations using FITC filter (Ex/Em = 490/525 nm).


     

  • Notes

    ab112125 should be stored dessicated.

  • Platform
    Fluorescence microscope

Properties

Images

  • Left Panel: Cells labeled with 1X Green Fluorescent Phalloidin Conjugate for 30 minutes only.
    Right Panel: Cells treated with phalloidin for 10 minutes, then stained with Green Fluorescent Phalloidin Conjugate for 30 minutes.

References

This product has been referenced in:
  • Banni M  et al. Assessing the impact of Benzo[a]pyrene on Marine Mussels: Application of a novel targeted low density microarray complementing classical biomarker responses. PLoS One 12:e0178460 (2017). Read more (PubMed: 28651000) »
See 1 Publication for this product

Customer reviews and Q&As

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Abreviews
The kit is easy to use. This is an advantage, though the fact that it comes with a buffer to use for the fixation protocol does not allow for easy versatility. Is a bit dis courageous to try using it adding other fluoroscopes and knowing which buffer suits the other fluorophores best.
For STED purposes, it could be used, but we found a bit higher the background noise. This might be as well the buffer, we did not test it further.
We used not very high STED power and work, but not being better than other dyes we are used to employ for actin staining.

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Verified customer

Submitted Mar 03 2015

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