F-actin Staining Kit - Orange Fluorescence - Cytopainter (ab112126)
Key features and details
- Assay type: Cell-based
- Platform: Fluorescence microscope
- Sample type: Adherent cells, Suspension cells, Tissue
Overview
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Product name
F-actin Staining Kit - Orange Fluorescence - Cytopainter
See all F-actin kits -
Sample type
Tissue, Adherent cells, Suspension cells -
Assay type
Cell-based -
Product overview
F-actin Staining Kit - Orange Fluorescence | Cytopainter (ab112126) fluorescence imaging kits are a set of fluorescence imaging tools for labeling sub-cellular organelles such as lysosomes, mitochondria, and actin filaments. The selective staining of cell compartments provides a powerful method for studying cellular events in a spatial and temporal context.
ab112126 is designed to stain F-actins in fixed cells with orange fluorescence. ab112126 uses an orange fluorescent phalloidin conjugate that is selectively bound to F-actins. The orange fluorescent phalloidin conjugate has Ex/Em = 550/575 nm. The conjugate also has good photostability for fluorescence imaging. It is a high-affinity probe for labeling, identifying and quantitating F-actins in formaldehyde-fixed and permeabilized tissue sections, cell cultures or cell-free experiments. ab112126 provides all the essential components with an optimized labeling protocol.
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Notes
ab112126 should be stored dessicated.
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Platform
Fluorescence microscope
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 500 tests Labeling Buffer 1 x 50ml Orange Fluorescent Phalloidin Conjugate 1 x 50µl -
Research areas
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Alternative names
- actin filament
- f actin
- Filamentous actin
Associated products
Images
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Functional Studies - CytoPainter F-actin Staining Kit - Orange Fluorescence (ab112126)ab112126, staining CPA cells fixed with formaldehyde in a black 96-well plate.
Left panel: The cells were labeled with Orange Fluorescent Phalloidin Conjugate for 30 minutes only.
Right panel: The cells were treated with phalloidin for 10 minutes, then stained with Orange Fluorescent Phalloidin Conjugate for 30 minutes.
References (1)
ab112126 has been referenced in 1 publication.
- Rowbotham SP et al. H3K9 methyltransferases and demethylases control lung tumor-propagating cells and lung cancer progression. Nat Commun 9:4559 (2018). PubMed: 30455465
