F-actin Staining Kit - Orange Fluorescence - Cytopainter (ab112126)

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Functional Studies - CytoPainter F-actin Staining Kit - Orange Fluorescence (ab112126)

    Key features and details

    • Assay type: Cell-based
    • Platform: Fluorescence microscope
    • Sample type: Adherent cells, Suspension cells, Tissue

    Overview

    • Product name

      F-actin Staining Kit - Orange Fluorescence - Cytopainter
      See all F-actin kits

    • Sample type

      Tissue, Adherent cells, Suspension cells

    • Assay type

      Cell-based

    • Product overview

      F-actin Staining Kit - Orange Fluorescence | Cytopainter (ab112126) fluorescence imaging kits are a set of fluorescence imaging tools for labeling sub-cellular organelles such as lysosomes, mitochondria, and actin filaments. The selective staining of cell compartments provides a powerful method for studying cellular events in a spatial and temporal context.


      ab112126 is designed to stain F-actins in fixed cells with orange fluorescence. ab112126 uses an orange fluorescent phalloidin conjugate that is selectively bound to F-actins. The orange fluorescent phalloidin conjugate has Ex/Em = 550/575 nm. The conjugate also has good photostability for fluorescence imaging. It is a high-affinity probe for labeling, identifying and quantitating F-actins in formaldehyde-fixed and permeabilized tissue sections, cell cultures or cell-free experiments. ab112126 provides all the essential components with an optimized labeling protocol.


       

    • Notes

      ab112126 should be stored dessicated.

    • Platform

      Fluorescence microscope

    Properties

    Images

    • Functional Studies - CytoPainter F-actin Staining Kit - Orange Fluorescence (ab112126)
      Functional Studies - CytoPainter F-actin Staining Kit - Orange Fluorescence (ab112126)
      ab112126, staining CPA cells fixed with formaldehyde in a black 96-well plate.
      Left panel: The cells were labeled with Orange Fluorescent Phalloidin Conjugate for 30 minutes only.
      Right panel: The cells were treated with phalloidin for 10 minutes, then stained with Orange Fluorescent Phalloidin Conjugate for 30 minutes.

    References (1)

    Publishing research using ab112126? Please let us know so that we can cite the reference in this datasheet.

    ab112126 has been referenced in 1 publication.

    • Rowbotham SP  et al. H3K9 methyltransferases and demethylases control lung tumor-propagating cells and lung cancer progression. Nat Commun 9:4559 (2018). PubMed: 30455465

    Customer reviews and Q&As

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    Question

    Can I use these kits along with traditional primary and secondary antibodies for staining?

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    Answer

    Thank you for contacting us.

    The CytoPainter kits are compatible with secondary antibodies and nuclear dyes for multicolor staining or GFP-transfected cells, for example. The lab has confirmed that these kits can be used with other antibodies providing that their emissions do not overlap with the kit's emission.

    https://www.abcam.com/index.html?pageconfig=resource&rid=14270

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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