Overview

  • Product name
    F-actin Staining Kit - Orange Fluorescence - Cytopainter
    See all F-actin kits
  • Sample type
    Tissue, Adherent cells, Suspension cells
  • Assay type
    Cell-based
  • Product overview

    F-actin Staining Kit - Orange Fluorescence | Cytopainter (ab112126) fluorescence imaging kits are a set of fluorescence imaging tools for labeling sub-cellular organelles such as lysosomes, mitochondria, and actin filaments. The selective staining of cell compartments provides a powerful method for studying cellular events in a spatial and temporal context.


    ab112126 is designed to stain F-actins in fixed cells with orange fluorescence. ab112126 uses an orange fluorescent phalloidin conjugate that is selectively bound to F-actins. The orange fluorescent phalloidin conjugate has Ex/Em = 550/575 nm. The conjugate also has good photostability for fluorescence imaging. It is a high-affinity probe for labeling, identifying and quantitating F-actins in formaldehyde-fixed and permeabilized tissue sections, cell cultures or cell-free experiments. ab112126 provides all the essential components with an optimized labeling protocol.


     

  • Notes

    ab112126 should be stored dessicated.

  • Platform
    Fluorescence microscope

Properties

Images

  • ab112126, staining CPA cells fixed with formaldehyde in a black 96-well plate.
    Left panel: The cells were labeled with Orange Fluorescent Phalloidin Conjugate for 30 minutes only.
    Right panel: The cells were treated with phalloidin for 10 minutes, then stained with Orange Fluorescent Phalloidin Conjugate for 30 minutes.

Protocols

References

This product has been referenced in:
  • Rowbotham SP  et al. H3K9 methyltransferases and demethylases control lung tumor-propagating cells and lung cancer progression. Nat Commun 9:4559 (2018). Read more (PubMed: 30455465) »
See 1 Publication for this product

Customer reviews and Q&As

Question
Answer

Thank you for contacting us.

The CytoPainter kits are compatible with secondary antibodies and nuclear dyes for multicolor staining or GFP-transfected cells, for example. The lab has confirmed that these kits can be used with other antibodies providing that their emissions do not overlap with the kit's emission.

https://www.abcam.com/index.html?pageconfig=resource&rid=14270

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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