F-actin Staining Kit - Orange Fluorescence - Cytopainter (ab112126)

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Overview

  • Product name
    F-actin Staining Kit - Orange Fluorescence - Cytopainter
    See all F-actin kits
  • Sample type
    Tissue, Adherent cells, Suspension cells
  • Assay type
    Cell-based
  • Product overview

    F-actin Staining Kit - Orange Fluorescence | Cytopainter (ab112126) fluorescence imaging kits are a set of fluorescence imaging tools for labeling sub-cellular organelles such as lysosomes, mitochondria, and actin filaments. The selective staining of cell compartments provides a powerful method for studying cellular events in a spatial and temporal context.


    ab112126 is designed to stain F-actins in fixed cells with orange fluorescence. ab112126 uses an orange fluorescent phalloidin conjugate that is selectively bound to F-actins. The orange fluorescent phalloidin conjugate has Ex/Em = 550/575 nm. The conjugate also has good photostability for fluorescence imaging. It is a high-affinity probe for labeling, identifying and quantitating F-actins in formaldehyde-fixed and permeabilized tissue sections, cell cultures or cell-free experiments. ab112126 provides all the essential components with an optimized labeling protocol.


     

  • Notes

    ab112126 should be stored dessicated.

  • Tested applications
    Suitable for: FM, ICC/IFmore details

Properties

Applications

Our Abpromise guarantee covers the use of ab112126 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
FM Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.

Images

  • Functional Studies - CytoPainter F-actin Staining Kit - Orange Fluorescence (ab112126)
    Functional Studies - CytoPainter F-actin Staining Kit - Orange Fluorescence (ab112126)
    ab112126, staining CPA cells fixed with formaldehyde in a black 96-well plate.
    Left panel: The cells were labeled with Orange Fluorescent Phalloidin Conjugate for 30 minutes only.
    Right panel: The cells were treated with phalloidin for 10 minutes, then stained with Orange Fluorescent Phalloidin Conjugate for 30 minutes.

Protocols

References

ab112126 has not yet been referenced specifically in any publications.

Publishing research using ab112126? Please let us know so that we can cite the reference in this datasheet.

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Can I use these kits along with traditional primary and secondary antibodies for staining?

Thank you for contacting us.

The CytoPainter kits are compatible with secondary antibodies and nuclear dyes for multicolor staining or GFP-transfected cells, for example. The lab has confirmed that these kits can be used with other antibodies...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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