Question (69671) | F-actin Staining Kit - Red Fluorescence - Cytopainter (ab112127)

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Question

I need to perform IF for Bone marrow stem cells. I need to do multiple staining such as "Vinculin" and F-actin. Can I use this kit along with another primary antibody? I want to incubate the primary antibody overnight at 4 degree C and use the F-actin along with the secondary antibody incubation. Could you please let me know if it should be a correct procedure.

Answer

Thank you for contacting us.

This kit has an excitation of 583 nm and an emission at 603 nm. I have contacted the lab who confirm that this kit can be used with other antibodies providing that its emission does not overlap with the kits emission.

I recommend using an Alexa488 conjugated secondary antibody against the primary antibody that you wish to use in conjugation with this kit.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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