Cytopainter F-actin in Hydractinia

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We used cytopainter F-actin staining kit to study morphology in the model organism Hydractinia during head regeneration. We fixed our whole animals in 4% pararfomaldehde in PBS followed by three ten minute washes in 0.1%triton in PBS. The animals were incubated in component in at a 1:500 dilution in component B for 45 minutes. They were then washed in PBS three times and mounted in fluorescent mounting medium.
The picture shows F-actin staining(Red) and Hoescht nuclear staining(blue). The image was taken on a spinning disc confocal microscope.

Mr. James Gahan

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Submitted Jan 23 2014

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