Key features and details
- Rat monoclonal [BM8] to F4/80
- Suitable for: ICC, Flow Cyt, IHC-Fr
- Reacts with: Mouse
- Isotype: IgG2a
Product nameAnti-F4/80 antibody [BM8]
See all F4/80 primary antibodies
DescriptionRat monoclonal [BM8] to F4/80
SpecificityThe monoclonal antibody BM8 recognizes a 125 kDa extracellular macrophage membrane molecule, highly restricted to mature macrophage subpopulations residing in tissue. This antibody does not cross react with any of the following cell types from Mouse: granulocytes, mast cells, platelets, lymphocytes, fibroblasts or endothelial cells. Although several publications have used this antibody succesfully in human, we have been unable to obtain positive results in this species and so do not guarantee it.
Tested applicationsSuitable for: ICC, Flow Cyt, IHC-Frmore details
Unsuitable for: IHC-P
Species reactivityReacts with: Mouse
Tissue, cells or virus corresponding to Mouse F4/80.
- Mouse macrophages
ab16911 is the only macrophage marker that is able to distinguish non-destructive from destructive inflammation prcoesses in the pancreas. Furthermore it is a unique histological marker of the progression from peri-insulitis to beta-cell and diabetes in a mouse diabetes model.
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We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.02% Sodium azide
Constituents: PBS, 0.1% BSA
Concentration information loading...
PurityProtein G purified
Purification notesProvided as a 0.2µm filtered antibody solution.
Primary antibody notesab16911 is the only macrophage marker that is able to distinguish non-destructive from destructive inflammation prcoesses in the pancreas. Furthermore it is a unique histological marker of the progression from peri-insulitis to beta-cell and diabetes in a mouse diabetes model.
Our Abpromise guarantee covers the use of ab16911 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC||Use at an assay dependent concentration.|
(Methanol fixed cells)
ab18450 - Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
See Schaller et al.
Fixation with acetone for 10 min at RT is recommended as is an incubation with 0.02 M sodium azide in PBS containing 0.1 % H2O2 for 10 min at RT to destroy endogenous peroxidase
FunctionOrphan receptor involved in cell adhesion and probably in cell-cell interactions specifically involving cells of the immune system. May play a role in regulatory T-cells (Treg) development.
Tissue specificityExpression is restricted to eosinophils.
Sequence similaritiesBelongs to the G-protein coupled receptor 2 family. Adhesion G-protein coupled receptor (ADGR) subfamily.
Contains 6 EGF-like domains.
Contains 1 GPS domain.
Cellular localizationCell membrane.
- Information by UniProt
- ADGRE1 antibody
- Adhesion G protein coupled receptor E1 antibody
- Adhesion G protein-coupled receptor E1 antibody
Detection of F4/80 in RAW cells. Red, black and blue line represent the isotype control, cells only and ab16911 at 10 μg/ml, respectively.
ab16911 staining F4/80 on macrophages in mouse liver tissue by Immunohistochemistry (Frozen sections).
ab16911 staining F4/80 in Mouse brain cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with acetone and blocked with 5% BSA for 1 hour at 20°C. Samples were incubated with primary antibody (1/250) for 16 hours at 4°C. An Alexa Fluor®568-conjugated Goat anti-rat IgG polyclonal (1/1000) was used as the secondary antibody.
Overlay histogram showing HeLa cells stained with ab16911 (red line). The cells were fixed with methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab16911, 1/10 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rat IgG (Fc) (ab96971) at 1/250 dilution for 30 min at 22°C. Isotype control antibody (black line) was rat IgG2a [aRTK2758] (ab18450, 2µg/1x106 cells cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a significantly decreased signal in HeLa cells fixed with 4% paraformaldehyde (10 min) used under the same conditions.
Please note that Abcam does not have data for use of this antibody on non-fixed cells. We welcome any customer feedback.
ab16911 staining mouse spleen tissue sections by immunohistochemistry (frozen sections). Sections were paraformaldehyde fixed without permeabilization and blocked in 1% serum for 10 minutes at 20°C. The primary antibody was used undiluted and incubated with sample for 16 hour at 20°C. A Biotin conjugated goat polyclonal to rat Ig, diluted 1/500 was used as the secondary antibody.
ab16911 stained RAW246.7 cells. The cells were 100% methanol fixed for 5 minutes at -20°C and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1hour at room temperature to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab16911 at 1in50 dilution) overnight at +4°C. The secondary antibody (pseudo-colored green) was Goat Anti-Rat IgG H&L (Alexa Fluor® 488) preadsorbed (ab150165) used at a 1/1000 dilution for 1hour at room temperature. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1hour at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.
ab16911 has been referenced in 73 publications.
- Tashita C et al. Kynurenine plays an immunosuppressive role in 2,4,6-trinitrobenzene sulfate-induced colitis in mice. World J Gastroenterol 26:918-932 (2020). PubMed: 32206003
- Yamasuge W et al. Indoleamine 2,3-dioxygenase 2 depletion suppresses tumor growth in a mouse model of Lewis lung carcinoma. Cancer Sci 110:3061-3067 (2019). PubMed: 31444833
- Wang Y et al. 18F-labeled magnetic nanoparticles for monitoring anti-angiogenic therapeutic effects in breast cancer xenografts. J Nanobiotechnology 17:105 (2019). PubMed: 31604441
- Sehl OC et al. Trimodal Cell Tracking In Vivo: Combining Iron- and Fluorine-Based Magnetic Resonance Imaging with Magnetic Particle Imaging to Monitor the Delivery of Mesenchymal Stem Cells and the Ensuing Inflammation. Tomography 5:367-376 (2019). PubMed: 31893235
- Hu Y et al. Restoration of p53 acetylation by HDAC inhibition permits the necrosis/apoptosis switch of pancreatic ainar cell during experimental pancreatitis in mice. J Cell Physiol 234:21988-21998 (2019). PubMed: 31058328