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Anti-F4/80 antibody [BM8] (ab16911)

Reviews (12)Q&A (7)References (110)
Flow Cytometry - Anti-F4/80 antibody [BM8] (ab16911)
  • Immunohistochemistry (Frozen sections) - Anti-F4/80 antibody [BM8] (ab16911)
  • Immunocytochemistry - Anti-F4/80 antibody [BM8] (ab16911)
  • Flow Cytometry - Anti-F4/80 antibody [BM8] (ab16911)
  • Immunohistochemistry (Frozen sections) - Anti-F4/80 antibody [BM8] (ab16911)
  • Immunocytochemistry - Anti-F4/80 antibody [BM8] (ab16911)

Key features and details

  • Rat monoclonal [BM8] to F4/80
  • Suitable for: ICC, Flow Cyt, IHC-Fr
  • Reacts with: Mouse, Human
  • Isotype: IgG2a

Conjugates logo Related conjugates and formulations

Biotin FITC PerCP

Overview

  • Product name

    Anti-F4/80 antibody [BM8]
    See all F4/80 primary antibodies

  • Description

    Rat monoclonal [BM8] to F4/80

  • Host species

    Rat

  • Specificity

    The monoclonal antibody BM8 recognizes a 125 kDa extracellular macrophage membrane molecule, highly restricted to mature macrophage subpopulations residing in tissue. This antibody does not cross react with any of the following cell types from Mouse: granulocytes, mast cells, platelets, lymphocytes, fibroblasts or endothelial cells.

  • Tested applications

    Suitable for: ICC, Flow Cyt, IHC-Frmore details

  • Species reactivity

    Reacts with: Mouse, Human

  • Immunogen

    Tissue, cells or virus corresponding to Mouse F4/80. BALB/c macrophages obtained from 14-day-old bone marrow cell cultures

  • Positive control

    • Flow Cyt: RAW and HeLa cells. IHC-Fr: Mouse liver and spleen tissues. ICC: Mouse brain and RAW246.7 cells.

  • General notes

    ab16911 is the only macrophage marker that is able to distinguish non-destructive from destructive inflammation prcoesses in the pancreas. Furthermore it is a unique histological marker of the progression from peri-insulitis to beta-cell and diabetes in a mouse diabetes model.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

  • Storage buffer

    Preservative: 0.02% Sodium azide
    Constituents: PBS, 0.1% BSA
  • Concentration information loading...

  • Purity

    Protein G purified

  • Purification notes

    Provided as a 0.2µm filtered antibody solution.

  • Primary antibody notes

    ab16911 is the only macrophage marker that is able to distinguish non-destructive from destructive inflammation prcoesses in the pancreas. Furthermore it is a unique histological marker of the progression from peri-insulitis to beta-cell and diabetes in a mouse diabetes model.

  • Clonality

    Monoclonal

  • Clone number

    BM8

  • Isotype

    IgG2a

  • Research areas

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab16911 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC (3)
Use at an assay dependent concentration.
Flow Cyt
1/50.

(Methanol fixed cells)

ab18450 - Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
IHC-Fr (3)
1/50.

See Schaller et al.

Fixation with acetone for 10 min at RT is recommended as is an incubation with 0.02 M sodium azide in PBS containing 0.1 % H2O2 for 10 min at RT to destroy endogenous peroxidase
Notes
ICC
Use at an assay dependent concentration.
Flow Cyt
1/50.

(Methanol fixed cells)

ab18450 - Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
IHC-Fr
1/50.

See Schaller et al.

Fixation with acetone for 10 min at RT is recommended as is an incubation with 0.02 M sodium azide in PBS containing 0.1 % H2O2 for 10 min at RT to destroy endogenous peroxidase

Target

  • Function

    Orphan receptor involved in cell adhesion and probably in cell-cell interactions specifically involving cells of the immune system. May play a role in regulatory T-cells (Treg) development.

  • Tissue specificity

    Expression is restricted to eosinophils.

  • Sequence similarities

    Belongs to the G-protein coupled receptor 2 family. Adhesion G-protein coupled receptor (ADGR) subfamily.
    Contains 6 EGF-like domains.
    Contains 1 GPS domain.

  • Cellular localization

    Cell membrane.
  • Information by UniProt

  • Database links

    • Alternative names

      • ADGRE1 antibody
      • Adhesion G protein coupled receptor E1 antibody
      • Adhesion G protein-coupled receptor E1 antibody
      • AGRE1_HUMAN antibody
      • Cell surface glycoprotein EMR1 antibody
      • Cell surface glycoprotein F4/80 antibody
      • DD7A5 7 antibody
      • Egf like module containing mucin like hormone receptor like 1 antibody
      • Egf like module containing mucin like hormone receptor like sequence 1 antibody
      • EGF like module receptor 1 antibody
      • EGF TM7 antibody
      • EGF-like module receptor 1 antibody
      • EGF-like module-containing mucin-like hormone receptor-like 1 antibody
      • EGFTM7 antibody
      • EMR 1 antibody
      • EMR1 antibody
      • EMR1 hormone receptor antibody
      • Gpf480 antibody
      • Ly71 antibody
      • Lymphocyte antigen 71 antibody
      • TM7LN3 antibody
      see all

    Images

    • Flow Cytometry - Anti-F4/80 antibody [BM8] (ab16911)
      Flow Cytometry - Anti-F4/80 antibody [BM8] (ab16911)

      Detection of F4/80 in RAW cells. Red, black and blue line represent the isotype control, cells only and ab16911 at 10 μg/ml, respectively.

    • Immunohistochemistry (Frozen sections) - Anti-F4/80 antibody [BM8] (ab16911)
      Immunohistochemistry (Frozen sections) - Anti-F4/80 antibody [BM8] (ab16911)
      ab16911 staining F4/80 on macrophages in mouse liver tissue by Immunohistochemistry (Frozen sections).
    • Immunocytochemistry - Anti-F4/80 antibody [BM8] (ab16911)
      Immunocytochemistry - Anti-F4/80 antibody [BM8] (ab16911)This image is courtesy of an anonymous Abreview
      ab16911 staining F4/80 in Mouse brain cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with acetone and blocked with 5% BSA for 1 hour at 20°C. Samples were incubated with primary antibody (1/250) for 16 hours at 4°C. An Alexa Fluor®568-conjugated Goat anti-rat IgG polyclonal (1/1000) was used as the secondary antibody.

      See Abreview

    • Flow Cytometry - Anti-F4/80 antibody [BM8] (ab16911)
      Flow Cytometry - Anti-F4/80 antibody [BM8] (ab16911)
      Overlay histogram showing HeLa cells stained with ab16911 (red line). The cells were fixed with methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab16911, 1/10 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rat IgG (Fc) (ab96971) at 1/250 dilution for 30 min at 22°C. Isotype control antibody (black line) was rat IgG2a [aRTK2758] (ab18450, 2µg/1x106 cells cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a significantly decreased signal in HeLa cells fixed with 4% paraformaldehyde (10 min) used under the same conditions.

      Please note that Abcam does not have data for use of this antibody on non-fixed cells. We welcome any customer feedback.
    • Immunohistochemistry (Frozen sections) - Anti-F4/80 antibody [BM8] (ab16911)
      Immunohistochemistry (Frozen sections) - Anti-F4/80 antibody [BM8] (ab16911)This image is courtesy of an Abreview submitted by Miss Silke Vorwald
      ab16911 staining mouse spleen tissue sections by immunohistochemistry (frozen sections). Sections were paraformaldehyde fixed without permeabilization and blocked in 1% serum for 10 minutes at 20°C. The primary antibody was used undiluted and incubated with sample for 16 hour at 20°C. A Biotin conjugated goat polyclonal to rat Ig, diluted 1/500 was used as the secondary antibody.

      See Abreview

    • Immunocytochemistry - Anti-F4/80 antibody [BM8] (ab16911)
      Immunocytochemistry - Anti-F4/80 antibody [BM8] (ab16911)

      ab16911 stained RAW246.7 cells. The cells were 100% methanol fixed for 5 minutes at -20°C and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1hour at room temperature to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab16911 at 1in50 dilution) overnight at +4°C. The secondary antibody (pseudo-colored green) was Goat Anti-Rat IgG H&L (Alexa Fluor® 488) preadsorbed (ab150165) used at a 1/1000 dilution for 1hour at room temperature. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1hour at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.

    References (110)

    Publishing research using ab16911? Please let us know so that we can cite the reference in this datasheet.

    ab16911 has been referenced in 110 publications.

    • Rojas Márquez JD  et al. Cutting Edge: NOX2 NADPH Oxidase Controls Infection by an Intracellular Bacterial Pathogen through Limiting the Type 1 IFN Response. J Immunol 206:323-328 (2021). PubMed: 33288542
    • Pan W  et al. ß-Glucan from Lentinula edodes prevents cognitive impairments in high-fat diet-induced obese mice: involvement of colon-brain axis. J Transl Med 19:54 (2021). PubMed: 33541370
    • Wieghofer P  et al. Mapping the origin and fate of myeloid cells in distinct compartments of the eye by single-cell profiling. EMBO J 40:e105123 (2021). PubMed: 33555074
    • Li J  et al. Omega-3FAs Can Inhibit the Inflammation and Insulin Resistance of Adipose Tissue Caused by HHcy Induced Lipids Profile Changing in Mice. Front Physiol 12:628122 (2021). PubMed: 33643070
    • Ang AD  et al. Gene and Protein Expression Is Altered by Ascorbate Availability in Murine Macrophages Cultured under Tumour-Like Conditions. Antioxidants (Basel) 10:N/A (2021). PubMed: 33799728
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-3 of 3 Abreviews or Q&A

    Immunocytochemistry abreview for Anti-F4/80 antibody [BM8]

     Excellent
    Abreviews
    abreview image
    Application
    Immunocytochemistry
    Sample
    Mouse Cell (Monocyte/Macrophages)
    Permeabilization
    Yes - 0.1% Triton X-100 in TBS for 5 to 10 minutes
    Specification
    Monocyte/Macrophages
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 37°C
    Fixative
    Formaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Dec 30 2020

    Immunocytochemistry abreview for Anti-F4/80 antibody [BM8]

     Good
    Abreviews
    abreview image
    Application
    Immunocytochemistry
    Sample
    Human Cell (Human Brain)
    Specification
    Human Brain
    Fixative
    Acetone
    Permeabilization
    No
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 18°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Sep 01 2010

    Immunocytochemistry abreview for Anti-F4/80 antibody [BM8]

     Good
    Abreviews
    abreview image
    Application
    Immunocytochemistry
    Sample
    Mouse Cell (Mouse Brain)
    Specification
    Mouse Brain
    Fixative
    Acetone
    Permeabilization
    No
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Sep 01 2010

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