Anti-F4/80 antibody [BM8.1] (Allophycocyanin) (ab210250)

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Overview

  • Product name

    Anti-F4/80 antibody [BM8.1] (Allophycocyanin)
    See all F4/80 primary antibodies

  • Description

    Rat monoclonal [BM8.1] to F4/80 (Allophycocyanin)

  • Host species

    Rat

  • Conjugation

    Allophycocyanin. Ex: 645nm, Em: 660nm

  • Tested applications

    Suitable for: Flow Cytmore details

  • Species reactivity

    Reacts with: Mouse

  • Immunogen

    The details of the immunogen for this antibody are not available.

  • Positive control

    • Mouse C57Bl/6 bone marrow cells

  • General notes

    ab210250 is widely used together with antibodies to CD115, CD11b and CD11c to identify myeloid / macrophage cells by flow cytometry.

    The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C. Store In the Dark.

  • Storage buffer

    pH: 7.2
    Preservative: 0.09% Sodium azide
    Constituents: 0.12% Monobasic dihydrogen sodium phosphate, 0.87% Sodium chloride, 0.1% Gelatin
  • Concentration information loading...

  • Purity

    Affinity purified

  • Purification notes

    ab210250 was purified from tissue culture supernatant.

  • Clonality

    Monoclonal

  • Clone number

    BM8.1

  • Isotype

    IgG2a

  • Light chain type

    kappa

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab210250 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent concentration.

Target

  • Function

    Orphan receptor involved in cell adhesion and probably in cell-cell interactions specifically involving cells of the immune system. May play a role in regulatory T-cells (Treg) development.

  • Tissue specificity

    Expression is restricted to eosinophils.

  • Sequence similarities

    Belongs to the G-protein coupled receptor 2 family. Adhesion G-protein coupled receptor (ADGR) subfamily.
    Contains 6 EGF-like domains.
    Contains 1 GPS domain.

  • Cellular localization

    Cell membrane.
  • Information by UniProt

  • Database links

    • Alternative names

      • ADGRE1 antibody
      • Adhesion G protein coupled receptor E1 antibody
      • Adhesion G protein-coupled receptor E1 antibody
      • AGRE1_HUMAN antibody
      • Cell surface glycoprotein EMR1 antibody
      • Cell surface glycoprotein F4/80 antibody
      • DD7A5 7 antibody
      • Egf like module containing mucin like hormone receptor like 1 antibody
      • Egf like module containing mucin like hormone receptor like sequence 1 antibody
      • EGF like module receptor 1 antibody
      • EGF TM7 antibody
      • EGF-like module receptor 1 antibody
      • EGF-like module-containing mucin-like hormone receptor-like 1 antibody
      • EGFTM7 antibody
      • EMR 1 antibody
      • EMR1 antibody
      • EMR1 hormone receptor antibody
      • Gpf480 antibody
      • Ly71 antibody
      • Lymphocyte antigen 71 antibody
      • TM7LN3 antibody
      see all

    Images

    • Flow Cytometry - Anti-F4/80 antibody [BM8.1] (Allophycocyanin) (ab210250)
      Flow Cytometry - Anti-F4/80 antibody [BM8.1] (Allophycocyanin) (ab210250)

      Flow cytometric analysis of F4/80 in mouse C57Bl/6 bone marrow cells using FITC Anti-Mouse CD11b and 0.5 μg ab210250 (right panel), or 0.5 μg Allophycocyanin Rat IgG2a isotype control (left panel).

    References

    ab210250 has not yet been referenced specifically in any publications.

    Publishing research using ab210250? Please let us know so that we can cite the reference in this datasheet.

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