Product nameAnti-F4/80 antibody - C-terminal
See all F4/80 primary antibodies
DescriptionChicken polyclonal to F4/80 - C-terminal
Tested applicationsSuitable for: ELISA, ICC/IF, WB, IHC-Pmore details
Species reactivityReacts with: Mouse
Predicted to work with: Rat, Human
Synthetic peptide corresponding to Mouse F4/80 (C terminal).
Database link: Q61549
- Mouse spleen lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 98% PBS, 1% Trehalose
Concentration information loading...
Purification notesCrude antiserum was purified by Thiopropyl Sepharose affinity chromatography.
Our Abpromise guarantee covers the use of ab186073 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ELISA||Use at an assay dependent concentration.|
|ICC/IF||Use at an assay dependent concentration.|
|WB||Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 102 kDa.|
|IHC-P||Use at an assay dependent concentration.|
FunctionOrphan receptor involved in cell adhesion and probably in cell-cell interactions specifically involving cells of the immune system. May play a role in regulatory T-cells (Treg) development.
Tissue specificityExpression is restricted to eosinophils.
Sequence similaritiesBelongs to the G-protein coupled receptor 2 family. Adhesion G-protein coupled receptor (ADGR) subfamily.
Contains 6 EGF-like domains.
Contains 1 GPS domain.
Cellular localizationCell membrane.
- Information by UniProt
- ADGRE1 antibody
- Adhesion G protein coupled receptor E1 antibody
- Adhesion G protein-coupled receptor E1 antibody
Anti-F4/80 antibody - C-terminal (ab186073) at 1 µg/ml + Mouse spleen lysate
Developed using the ECL technique.
Predicted band size: 102 kDa
Exposure time: 5 seconds
Chicken anti mouse F4/80 (ab186073, polyclonal) staining of mouse RAW cells by immunofluorescence, using a FITC-conjugated anti chicken antibody as a secondary antibody.
ab186073 has been referenced in 2 publications.
- Amini-Nik S et al. Accumulation of myeloid lineage cells is mapping out liver fibrosis post injury: a targetable lesion using Ketanserin. Exp Mol Med 50:81 (2018). PubMed: 30026607
- Secchi MF et al. Heparanase and macrophage interplay in the onset of liver fibrosis. Sci Rep 7:14956 (2017). PubMed: 29097791