For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome

We use cookies to make our site as useful as possible.

Our Cookie Policy explains how you can opt-out of the cookies we use.

If you continue without changing your cookie settings, we'll assume you’re happy with this.

Continue Continue

United States
Your country/region is currently set to:

If incorrect, please enter your country/region into the box below, to view site information related to your country/region.

Call (888) 77-ABCAM (22226) or contact us
Need help? Contact us

  • My account
  • Sign out
Sign in or Register with us

Welcome

Sign in or

Don't have an account?

Register with us
My basket
Quick order
Abcam homepage

  • Research Products
    By product type
    Primary antibodies
    Secondary antibodies
    ELISA and Matched Antibody Pair Kits
    Cell and tissue imaging tools
    Cellular and biochemical assays
    Proteins and Peptides
    By product type
    Proteomics tools
    Agonists, activators, antagonists and inhibitors
    Cell lines and Lysates
    Multiplex miRNA assays
    Multiplex Assays
    By research area
    Cancer
    Cardiovascular
    Cell Biology
    Epigenetics
    Metabolism
    Developmental Biology
    By research area
    Immunology
    Microbiology
    Neuroscience
    Signal Transduction
    Stem Cells
  • Diagnostic & Therapeutic Solutions
    Custom solutions & partnerships

    Custom antibody development and commercial partnerships to advance your diagnostic and therapeutic discovery.

    Create custom solutions with us

    Partner with us

  • Support
    Support hub

    Access advice and support for any research roadblock

    View support hub

    Protocols

    Your experiments laid out step by step

    View protocols

  • Events
    • Conference calendar
    • Cancer
    • Cardiovascular
    • Epigenetics & Nuclear signaling
    • Immunology
    • Neuroscience
    • Stem cells
    • Tradeshows
    • Scientific webinars
    Keep up to date with the latest events

    Full event breakdown with abstracts, speakers, registration and more

    View global event calendar

  • Pathways
    Cell signalling pathways

    View all pathways

    View all interactive pathways

Supporting our customers and employees during the COVID-19 pandemic. Read more

  1. Link

    f480-antibody-c-terminal-ab186073.pdf

  1. Send me a copy of this email
    I agree to the terms and conditions.
Immunology Innate Immunity Macrophage / Inflamm.
Share by email

Anti-F4/80 antibody - C-terminal (ab186073)

  • Datasheet
  • SDS
Reviews (2) Submit a question References (4)

Product price, shipping and contact information

Currently unavailable

Sorry, we can't display this right now.

Please contact us to place your order, or try again later.

 

Loading size & price…

 

Shipping and order information

Abpromise

Guaranteed product quality, expert customer support.

Find out more.

Western blot - Anti-F4/80 antibody - C-terminal (ab186073)
  • Immunocytochemistry/ Immunofluorescence - Anti-F4/80 antibody - C-terminal (ab186073)

Key features and details

  • Chicken polyclonal to F4/80 - C-terminal
  • Suitable for: ICC/IF, WB
  • Reacts with: Mouse
  • Isotype: IgY

You may also be interested in

Protein
Product image
Recombinant Human EMR1/ADGRE1 protein (ab131740)
Secondary
Product image
Goat Anti-Chicken IgY H&L (Alexa Fluor® 488) (ab150169)
Knockout
Product image
Human EMR1 (F4/80) knockout HeLa cell line (ab265670)

View more associated products

Overview

  • Product name

    Anti-F4/80 antibody - C-terminal
    See all F4/80 primary antibodies
  • Description

    Chicken polyclonal to F4/80 - C-terminal
  • Host species

    Chicken
  • Tested applications

    Suitable for: ICC/IF, WBmore details
  • Species reactivity

    Reacts with: Mouse
    Predicted to work with: Rat, Human
  • Immunogen

    Synthetic peptide corresponding to Mouse F4/80 (C terminal).
    Database link: Q61549

  • Positive control

    • Mouse spleen lysate.
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 98% PBS, 1% Trehalose
  • Concentration information loading...
  • Purity

    IgY fraction
  • Purification notes

    Crude antiserum was purified by Thiopropyl Sepharose affinity chromatography.
  • Clonality

    Polyclonal
  • Isotype

    IgY
  • Research areas

    • Immunology
    • Innate Immunity
    • Macrophage / Inflamm.
    • Tags & Cell Markers
    • Cell Type Markers
    • Other Cell Types

Associated products

  • Conjugation kits

    • PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918)
    • APC Conjugation Kit - Lightning-Link® (ab201807)
  • Isotype control

    • Chicken IgY - Isotype Control (ab50579)
  • Positive Controls

    • Mouse spleen tissue lysate - total protein (ab29293)
  • Recombinant Protein

    • Recombinant Human EMR1/ADGRE1 protein (ab131740)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab186073 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
Use at an assay dependent concentration.
WB
Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 102 kDa.
Notes
ICC/IF
Use at an assay dependent concentration.
WB
Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 102 kDa.

Target

  • Function

    Orphan receptor involved in cell adhesion and probably in cell-cell interactions specifically involving cells of the immune system. May play a role in regulatory T-cells (Treg) development.
  • Tissue specificity

    Expression is restricted to eosinophils.
  • Sequence similarities

    Belongs to the G-protein coupled receptor 2 family. Adhesion G-protein coupled receptor (ADGR) subfamily.
    Contains 6 EGF-like domains.
    Contains 1 GPS domain.
  • Cellular localization

    Cell membrane.
  • Target information above from: UniProt accession Q14246 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 2015 Human
    • Entrez Gene: 13733 Mouse
    • Entrez Gene: 316137 Rat
    • Omim: 600493 Human
    • SwissProt: Q14246 Human
    • SwissProt: Q61549 Mouse
    • SwissProt: Q5Y4N8 Rat
    • Unigene: 2375 Human
    • Unigene: 2254 Mouse
    see all
  • Alternative names

    • ADGRE1 antibody
    • Adhesion G protein coupled receptor E1 antibody
    • Adhesion G protein-coupled receptor E1 antibody
    • AGRE1_HUMAN antibody
    • Cell surface glycoprotein EMR1 antibody
    • Cell surface glycoprotein F4/80 antibody
    • DD7A5 7 antibody
    • Egf like module containing mucin like hormone receptor like 1 antibody
    • Egf like module containing mucin like hormone receptor like sequence 1 antibody
    • EGF like module receptor 1 antibody
    • EGF TM7 antibody
    • EGF-like module receptor 1 antibody
    • EGF-like module-containing mucin-like hormone receptor-like 1 antibody
    • EGFTM7 antibody
    • EMR 1 antibody
    • EMR1 antibody
    • EMR1 hormone receptor antibody
    • Gpf480 antibody
    • Ly71 antibody
    • Lymphocyte antigen 71 antibody
    • TM7LN3 antibody
    see all

Images

  • Western blot - Anti-F4/80 antibody - C-terminal (ab186073)
    Western blot - Anti-F4/80 antibody - C-terminal (ab186073)
    Anti-F4/80 antibody - C-terminal (ab186073) at 1 µg/ml + Mouse spleen lysate

    Developed using the ECL technique.

    Predicted band size: 102 kDa


    Exposure time: 5 seconds
  • Immunocytochemistry/ Immunofluorescence - Anti-F4/80 antibody - C-terminal (ab186073)
    Immunocytochemistry/ Immunofluorescence - Anti-F4/80 antibody - C-terminal (ab186073)

    Chicken anti mouse F4/80 (ab186073, polyclonal) staining of mouse RAW cells by immunofluorescence, using a FITC-conjugated anti chicken antibody as a secondary antibody.

Protocols

  • Immunohistochemistry protocols
  • Immunocytochemistry & immunofluorescence protocols
  • Western blot protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (4)

Publishing research using ab186073? Please let us know so that we can cite the reference in this datasheet.

ab186073 has been referenced in 4 publications.

  • Andreichenko IN  et al. 4-methylumbelliferone Prevents Liver Fibrosis by Affecting Hyaluronan Deposition, FSTL1 Expression and Cell Localization. Int J Mol Sci 20:N/A (2019). PubMed: 31847129
  • Amini-Nik S  et al. Accumulation of myeloid lineage cells is mapping out liver fibrosis post injury: a targetable lesion using Ketanserin. Exp Mol Med 50:81 (2018). PubMed: 30026607
  • Masola V  et al. Heparanase regulates the M1 polarization of renal macrophages and their crosstalk with renal epithelial tubular cells after ischemia/reperfusion injury. FASEB J 32:742-756 (2018). PubMed: 28970256
  • Secchi MF  et al. Heparanase and macrophage interplay in the onset of liver fibrosis. Sci Rep 7:14956 (2017). PubMed: 29097791

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

1-2 of 2 Abreviews or Q&A

Immunohistochemistry (Frozen sections) abreview for Anti-F4/80 antibody - C-terminal

Inconclusive
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (gastrocnemius muscle)
Permeabilization
No
Specification
gastrocnemius muscle
Blocking step
Dako / Protein Block / X0909 as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 22°C
Fixative
nothing
Read More

Abcam user community

Verified customer

Submitted Nov 25 2016

Immunohistochemistry (Frozen sections) abreview for Anti-F4/80 antibody - C-terminal

Poor
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Liver)
Permeabilization
No
Specification
Liver
Blocking step
BSA as blocking agent for 15 minute(s) · Concentration: 1% · Temperature: 22°C
Fixative
Paraformaldehyde
Read More

Abcam user community

Verified customer

Submitted Dec 04 2015

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Get resources and offers direct to your inbox Sign up
A-Z by research area
  • Cancer
  • Cardiovascular
  • Cell biology
  • Developmental biology
  • Epigenetics & Nuclear signaling
  • Immunology
  • Metabolism
  • Microbiology
  • Neuroscience
  • Signal transduction
  • Stem cells
A-Z by product type
  • Primary antibodies
  • Secondary antibodies
  • Biochemicals
  • Isotype controls
  • Flow cytometry multi-color selector
  • Kits
  • Loading controls
  • Lysates
  • Peptides
  • Proteins
  • Slides
  • Tags and cell markers
  • Tools & Reagents
Help & support
  • Support
  • Make an Inquiry
  • Protocols & troubleshooting
  • Placing an order
  • RabMAb products
  • Biochemical product FAQs
  • Training
  • Browse by Target
Company
  • Corporate site
  • Investor relations
  • Company news
  • Careers
  • About us
  • Blog
Events
  • Tradeshows
  • Conferences
International websites
  • abcam.cn
  • abcam.co.jp

Join with us

  • LinkedIn
  • facebook
  • Twitter
  • YouTube
  • Terms of sale
  • Website terms of use
  • Cookie policy
  • Privacy policy
  • Legal
  • Modern slavery statement
© 1998-2021 Abcam plc. All rights reserved.