Anti-F4/80 antibody - C-terminal (ab186073)
- Datasheet
- Specific References (1)
- Protocols
Overview
-
Product nameAnti-F4/80 antibody - C-terminal
See all F4/80 primary antibodies -
DescriptionChicken polyclonal to F4/80 - C-terminal
-
Host speciesChicken
-
Tested applicationsSuitable for: ELISA, ICC/IF, WB, IHC-Pmore details
-
Species reactivityReacts with: Mouse
Predicted to work with: Rat, Human
-
Immunogen
Synthetic peptide corresponding to Mouse F4/80 (C terminal).
Database link: Q61549 -
Positive control
- Mouse spleen lysate.
Properties
-
FormLiquid
-
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
-
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 98% PBS, 1% Trehalose -
Concentration information loading... -
PurityIgY fraction
-
Purification notesCrude antiserum was purified by Thiopropyl Sepharose affinity chromatography.
-
ClonalityPolyclonal
-
IsotypeIgY
-
Research areas
Associated products
-
Positive Controls
Applications
Our Abpromise guarantee covers the use of ab186073 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ELISA | Use at an assay dependent concentration. | |
| ICC/IF | Use at an assay dependent concentration. | |
| WB | Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 102 kDa. | |
| IHC-P | Use at an assay dependent concentration. |
Target
-
FunctionOrphan receptor involved in cell adhesion and probably in cell-cell interactions specifically involving cells of the immune system. May play a role in regulatory T-cells (Treg) development.
-
Tissue specificityExpression is restricted to eosinophils.
-
Sequence similaritiesBelongs to the G-protein coupled receptor 2 family. Adhesion G-protein coupled receptor (ADGR) subfamily.
Contains 6 EGF-like domains.
Contains 1 GPS domain. -
Cellular localizationCell membrane.
- Information by UniProt
-
Database links
- Entrez Gene: 2015 Human
- Entrez Gene: 13733 Mouse
- Entrez Gene: 316137 Rat
- Omim: 600493 Human
- SwissProt: Q14246 Human
- SwissProt: Q61549 Mouse
- SwissProt: Q5Y4N8 Rat
- Unigene: 2375 Human
see all -
Alternative names
- ADGRE1 antibody
- Adhesion G protein coupled receptor E1 antibody
- Adhesion G protein-coupled receptor E1 antibody
see all
Images
-
Western blot - Anti-F4/80 antibody - C-terminal (ab186073)Anti-F4/80 antibody - C-terminal (ab186073) at 1 µg/ml + Mouse spleen lysate
Developed using the ECL technique.
Predicted band size: 102 kDa
Exposure time: 5 seconds -
Immunocytochemistry/ Immunofluorescence - Anti-F4/80 antibody - C-terminal (ab186073)Chicken anti mouse F4/80 (ab186073, polyclonal) staining of mouse RAW cells by immunofluorescence, using a FITC-conjugated anti chicken antibody as a secondary antibody.
Protocols
References
This product has been referenced in:
- Secchi MF et al. Heparanase and macrophage interplay in the onset of liver fibrosis. Sci Rep 7:14956 (2017). Read more (PubMed: 29097791) »
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"