Anti-F4/80 antibody - C-terminal (ab186073)
Overview
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Product name
Anti-F4/80 antibody - C-terminal
See all F4/80 primary antibodies -
Description
Chicken polyclonal to F4/80 - C-terminal -
Host species
Chicken -
Tested applications
Suitable for: ELISA, ICC/IF, WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse
Predicted to work with: Rat, Human
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Immunogen
Synthetic peptide corresponding to Mouse F4/80 (C terminal).
Database link: Q61549 -
Positive control
- Mouse spleen lysate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 98% PBS, 1% Trehalose -
Concentration information loading... -
Purity
IgY fraction -
Purification notes
Crude antiserum was purified by Thiopropyl Sepharose affinity chromatography. -
Clonality
Polyclonal -
Isotype
IgY -
Research areas
Associated products
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Conjugation kits
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab186073 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ELISA | Use at an assay dependent concentration. | |
| ICC/IF | Use at an assay dependent concentration. | |
| WB | Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 102 kDa. | |
| IHC-P | Use at an assay dependent concentration. |
Target
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Function
Orphan receptor involved in cell adhesion and probably in cell-cell interactions specifically involving cells of the immune system. May play a role in regulatory T-cells (Treg) development. -
Tissue specificity
Expression is restricted to eosinophils. -
Sequence similarities
Belongs to the G-protein coupled receptor 2 family. Adhesion G-protein coupled receptor (ADGR) subfamily.
Contains 6 EGF-like domains.
Contains 1 GPS domain. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 2015 Human
- Entrez Gene: 13733 Mouse
- Entrez Gene: 316137 Rat
- Omim: 600493 Human
- SwissProt: Q14246 Human
- SwissProt: Q61549 Mouse
- SwissProt: Q5Y4N8 Rat
- Unigene: 2375 Human
see all -
Alternative names
- ADGRE1 antibody
- Adhesion G protein coupled receptor E1 antibody
- Adhesion G protein-coupled receptor E1 antibody
see all
Images
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Western blot - Anti-F4/80 antibody - C-terminal (ab186073)Anti-F4/80 antibody - C-terminal (ab186073) at 1 µg/ml + Mouse spleen lysate
Developed using the ECL technique.
Predicted band size: 102 kDa
Exposure time: 5 seconds -
Immunocytochemistry/ Immunofluorescence - Anti-F4/80 antibody - C-terminal (ab186073)Chicken anti mouse F4/80 (ab186073, polyclonal) staining of mouse RAW cells by immunofluorescence, using a FITC-conjugated anti chicken antibody as a secondary antibody.
Protocols
Datasheets and documents
References
ab186073 has been referenced in 2 publications.
- Amini-Nik S et al. Accumulation of myeloid lineage cells is mapping out liver fibrosis post injury: a targetable lesion using Ketanserin. Exp Mol Med 50:81 (2018). PubMed: 30026607
- Secchi MF et al. Heparanase and macrophage interplay in the onset of liver fibrosis. Sci Rep 7:14956 (2017). PubMed: 29097791
