Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-F4/80 antibody [CI:A3-1]

Good
Abreviews
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (mouse adipose tissue)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10mm sodium citrate Ph6.0
Permeabilization
No
Specification
mouse adipose tissue
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Fixative
Paraformaldehyde

Other product details

Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 2% BSA
Dilution
1/100

Secondary antibody

Dilution
1/400
Name
Non-Abcam antibody was used: Biotinylated Donkey anti rat
Host species: Donkey
Clonality: Polyclonal
Conjugation: Biotin

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Submitted Apr 15 2013

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