Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-F4/80 antibody [CI:A3-1]

Good
Abreviews
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Colon)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Diva Decloaker
Permeabilization
No
Specification
Colon
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 22°C
Fixative
Formaldehyde

Other product details

Incubation time
20 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: PBS+1% BSA
Dilution
1/100

Secondary antibody

Dilution
1/250
Name
Non-Abcam antibody was used: Donkey anti-Rat Alexa Fluor 647
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor­ 647

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Submitted Sep 06 2013

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