Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-F4/80 antibody [CI:A3-1]

Excellent
Abreviews
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate buffer
Sample
Mouse Tissue sections (adipose tissue)
Specification
adipose tissue
Permeabilization
No
Fixative
Paraformaldehyde

Other product details

Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution
1/500

Secondary antibody

Name
Non-Abcam antibody was used: Biotinylated anti- RAT igG(H+L)
Host species: Rat
Clonality: Polyclonal
Conjugation: Dual Color: APC + PE

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Submitted Dec 19 2013

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