Immunocytochemistry/ Immunofluorescence abreview for Anti-F4/80 antibody [CI:A3-1]

Excellent
Abreviews
Application
Immunocytochemistry/ Immunofluorescence
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
Sample
Mouse Cell (peritoneal macrophage)
Specification
peritoneal macrophage
Permeabilization
Yes - 0.2% Triton X100
Fixative
Formaldehyde

Other product details

Incubation time
1 hour(s) and 0 minute(s) · Temperature: 20°C · Diluent: 10% goat serum
Dilution
1/150

Secondary antibody

Name
Non-Abcam antibody was used: goat anti-mouse
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor­ 647
Dilution
1/350

Additional data

Additional Notes
Peritoneal macrophages were stained with anti-F4/80 antibody ab6640 (green) and counterstained with rhodamine phalloidin to label F-actin (red). The staining was very clean and showed F4/80 to be often together with F-actin at cell edges.

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Submitted Aug 19 2014

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