Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-F4/80 antibody [CI:A3-1]

Excellent
Abreviews
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 20°C
Antigen retrieval step
Enzymatic - Buffer/Enzyme Used: proteinaseK
Sample
Mouse Tissue sections (Brain)
Specification
Brain
Permeabilization
Yes - triton-x
Fixative
Paraformaldehyde

Other product details

Incubation time
24 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution
1/50

Secondary antibody

Name
Non-Abcam antibody was used: sigma anti-rat-IgG
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Biotin
Dilution
1/400

Miss. Cathy Wyse

Verified customer

Submitted May 28 2014

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