Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-F4/80 antibody [CI:A3-1]

Excellent
Abreviews
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (kidney)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate buffer 1X
Permeabilization
No
Specification
kidney
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2.5% · Temperature: 25°C
Fixative
Formaldehyde

Other product details

Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: PBS 1X + 3% of 2.5% horse serum
Dilution
1/500

Secondary antibody

Name
Non-Abcam antibody was used: immpress anti-rabbit
Host species: Horse
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase

Additional data

Additional Notes
Obese mouse - kidney tissue

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Submitted Oct 14 2015

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