Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-F4/80 antibody [CI:A3-1]

Good
Abreviews
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (skin)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate buffer pH=6
Permeabilization
No
Specification
skin
Blocking step
Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C
Fixative
10% formalin

Other product details

Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 10% goat serum
Dilution
1/100

Secondary antibody

Dilution
1/400
Name
Non-Abcam antibody was used: biotinylated goat anti-rat IgG
Host species: Goat
Clonality: Polyclonal
Conjugation: Biotin

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Submitted Feb 18 2016

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