Western blot abreview for Anti-F4/80 antibody [CI:A3-1]

Average
Abreviews
Application
Western blot
Sample
Human Cell lysate - whole cell (Human Macrophages)
Gel Running Conditions
Reduced Denaturing (12)
Loading amount
30 µg
Specification
Human Macrophages
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 26°C

Other product details

Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 2% Milk in PBS
Dilution
1/250

Secondary antibody

Dilution
1/10000
Name

Detection

Detection method
ECL
Exposure
2 minute(s) and 0 second(s)
Bands
Specific: 102 kDa

Additional data

Additional Notes
Please note that I tested this antibody on human macrophages. However, Abcam guarantees the antibody to react with Mouse Only.

Dr. Francesco Elia Marino

Verified customer

Submitted Apr 06 2016

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