Western blot abreview for Anti-F4/80 antibody [CI:A3-1]

Good
Abreviews
Application
Western blot
Sample
Mouse Tissue lysate - whole (Heart)
Gel Running Conditions
Reduced Denaturing (4-12%)
Loading amount
30 µg
Specification
Heart
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Other product details

Incubation time
14 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: TBST with 2.5% BSA
Dilution
1/1000

Secondary antibody

Dilution
1/3000
Name
Non-Abcam antibody was used: Polyclonal Rabbit Anti Rat Immunoglobulins/HRP
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase

Detection

Detection method
ECL
Exposure
5 second(s)
Bands
Specific: 120 kDa Non-specific: 200, 220 kDa
Positive control
This gene is expressed in the heart according to public microarray data.

Additional data

Additional Notes
This antibody has a strong background. Extra blocking time is recommended. Choose milk over BSA as blocking agent may reduce the background as well.

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Submitted Oct 04 2016

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