Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-F4/80 antibody [CI:A3-1]

Excellent
Abreviews
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Upper jaw)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10x Citrate Buffer pH 6.0 (ab64214)
Permeabilization
No
Specification
Upper jaw
Blocking step
Protein block (X0909, DAKO) as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C
Fixative
Paraformaldehyde

Other product details

Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: Antibody diluent (S0809, DAKO)
Dilution
1/50

Secondary antibody

Name
Non-Abcam antibody was used: MAX-PO (Rat) (414311, Nichirei Biosciences)
Host species: Rat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase

Additional data

PubMed ID
doi: 10.1111/jre.12578: View

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Submitted Feb 28 2019

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