Overview

  • Product name
    Anti-F4/80 antibody [CI:A3-1] (Allophycocyanin)
    See all F4/80 primary antibodies
  • Description
    Rat monoclonal [CI:A3-1] to F4/80 (Allophycocyanin)
  • Host species
    Rat
  • Conjugation
    Allophycocyanin. Ex: 645nm, Em: 660nm
  • Specificity
    Recognises the mouse F4/80 antigen.
  • Tested applications
    Suitable for: Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    Thioglycollate stimulated peritoneal cavity macrophages from C57/BL mice.

  • Positive control
    • Mouse peritoneal macrophages cells

Properties

Applications

Our Abpromise guarantee covers the use of ab105080 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 10µl for 106 cells.

of Neat - 1/10 diluted antibody in 100µl.

 

 

 

ab154434 - Rat monoclonal IgG2b, is suitable for use as an isotype control with this antibody.

 

Target

  • Function
    Orphan receptor involved in cell adhesion and probably in cell-cell interactions specifically involving cells of the immune system. May play a role in regulatory T-cells (Treg) development.
  • Tissue specificity
    Expression is restricted to eosinophils.
  • Sequence similarities
    Belongs to the G-protein coupled receptor 2 family. Adhesion G-protein coupled receptor (ADGR) subfamily.
    Contains 6 EGF-like domains.
    Contains 1 GPS domain.
  • Cellular localization
    Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • ADGRE1 antibody
    • Adhesion G protein coupled receptor E1 antibody
    • Adhesion G protein-coupled receptor E1 antibody
    • AGRE1_HUMAN antibody
    • Cell surface glycoprotein EMR1 antibody
    • Cell surface glycoprotein F4/80 antibody
    • DD7A5 7 antibody
    • Egf like module containing mucin like hormone receptor like 1 antibody
    • Egf like module containing mucin like hormone receptor like sequence 1 antibody
    • EGF like module receptor 1 antibody
    • EGF TM7 antibody
    • EGF-like module receptor 1 antibody
    • EGF-like module-containing mucin-like hormone receptor-like 1 antibody
    • EGFTM7 antibody
    • EMR 1 antibody
    • EMR1 hormone receptor antibody
    • Gpf480 antibody
    • Ly71 antibody
    • Lymphocyte antigen 71 antibody
    • TM7LN3 antibody
    see all

Images

  • Flow cytometry staining of mouse peritoneal macrophages cells with ab105080.
  • FSC/SSC gated mouse peritoneal macrophages dual stained with ab105080 at 1/5 dilution and anti-CD169 at a neat dilution by Flow cytometry. Isotype control pair in red. Fc receptors were blocked.

References

This product has been referenced in:
  • Combs MD  et al. Microfibril-associated glycoprotein 2 (MAGP2) loss of function has pleiotropic effects in vivo. J Biol Chem 288:28869-80 (2013). Flow Cyt ; Mouse . Read more (PubMed: 23963447) »
See 1 Publication for this product

Customer reviews and Q&As

Question
Answer

Thank you for contacting us.

The concentration of lot you have received is 1mg/ml.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
For licensing inquiries, please contact partnerships@abcam.com

Sign up