FITC Anti-F4/80 antibody [CI:A3-1] (ab105155)
Key features and details
- FITC Rat monoclonal [CI:A3-1] to F4/80
- Suitable for: Flow Cyt
- Reacts with: Mouse
- Conjugation: FITC. Ex: 493nm, Em: 528nm
- Isotype: IgG2b
Related conjugates and formulations
Overview
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Product name
FITC Anti-F4/80 antibody [CI:A3-1]
See all F4/80 primary antibodies -
Description
FITC Rat monoclonal [CI:A3-1] to F4/80 -
Host species
Rat -
Conjugation
FITC. Ex: 493nm, Em: 528nm -
Specificity
ab105155 recognises the murine F4/80 antigen. -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
Tissue, cells or virus corresponding to Mouse F4/80. Thioglycollate stimulated peritoneal macrophages from C57/BL mice.
Database link: Q61549 -
Positive control
- Mouse peritoneal macrophages
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General notes
Clone CI:A3-1 has been reported to modulate cytokine levels released in response to Listeria monocytogenes.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.40
Preservative: 0.09% Sodium azide
Constituents: 1% BSA, PBS -
Concentration information loading...
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Purity
Protein G purified -
Primary antibody notes
Clone CI:A3-1 has been reported to modulate cytokine levels released in response to Listeria monocytogenes. -
Clonality
Monoclonal -
Clone number
CI:A3-1 -
Myeloma
NS1 -
Isotype
IgG2b -
Light chain type
kappa -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab105155 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
Flow Cyt |
Use 10µl for 106 cells.
in 100ul. ab136125 - Rat monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
Notes |
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Flow Cyt
Use 10µl for 106 cells. in 100ul. ab136125 - Rat monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
Target
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Function
Orphan receptor involved in cell adhesion and probably in cell-cell interactions specifically involving cells of the immune system. May play a role in regulatory T-cells (Treg) development. -
Tissue specificity
Expression is restricted to eosinophils. -
Sequence similarities
Belongs to the G-protein coupled receptor 2 family. Adhesion G-protein coupled receptor (ADGR) subfamily.
Contains 6 EGF-like domains.
Contains 1 GPS domain. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 13733 Mouse
- SwissProt: Q61549 Mouse
- Unigene: 2254 Mouse
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Alternative names
- ADGRE1 antibody
- Adhesion G protein coupled receptor E1 antibody
- Adhesion G protein-coupled receptor E1 antibody
see all
Images
Protocols
Datasheets and documents
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Datasheet download
References (15)
ab105155 has been referenced in 15 publications.
- Chen Y et al. Prevotellaceae produces butyrate to alleviate PD-1/PD-L1 inhibitor-related cardiotoxicity via PPARα-CYP4X1 axis in colonic macrophages. J Exp Clin Cancer Res 41:1 (2022). PubMed: 34980222
- Stahl EC et al. Inflammation and Ectopic Fat Deposition in the Aging Murine Liver Is Influenced by CCR2. Am J Pathol 190:372-387 (2020). PubMed: 31843499
- Mohamad NE et al. Apoptosis and metastasis inhibitory potential of pineapple vinegar against mouse mammary gland cells in vitro and in vivo. Nutr Metab (Lond) 16:49 (2019). PubMed: 31372176
- Kim H et al. Exosome-Guided Phenotypic Switch of M1 to M2 Macrophages for Cutaneous Wound Healing. Adv Sci (Weinh) 6:1900513 (2019). PubMed: 31637157
- Rodríguez MM et al. 4Mu Decreases CD47 Expression on Hepatic Cancer Stem Cells and Primes a Potent Antitumor T Cell Response Induced by Interleukin-12. Mol Ther 26:2738-2750 (2018). PubMed: 30301668