Anti-F4/80 antibody [CI:A3-1] - Macrophage Marker (ab6640)
![Immunocytochemistry/ Immunofluorescence - Anti-F4/80 antibody [CI:A3-1] - Macrophage Marker (ab6640)](https://www.abcam.com/ps/products/6/ab6640/Images/ab6640-266714-anti-f4-80-antibody-cia3-1-immunofluorescence.jpg "Immunocytochemistry/ Immunofluorescence - Anti-F4/80 antibody [CI:A3-1] - Macrophage Marker (ab6640)")
Key features and details
- Rat monoclonal [CI:A3-1] to F4/80 - Macrophage Marker
- Suitable for: Flow Cyt, ICC/IF
- Reacts with: Mouse
- Isotype: IgG2b
Overview
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Product name
Anti-F4/80 antibody [CI:A3-1] - Macrophage Marker
See all F4/80 primary antibodies -
Description
Rat monoclonal [CI:A3-1] to F4/80 - Macrophage Marker -
Host species
Rat -
Specificity
Anti-F4/80 antibody (ab6640) recognises the mouse F4/80 antigen, a 160kD glycoprotein expressed by murine macrophages.
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Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt MouseICC/IF Mouse
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Immunogen
Thioglycollate stimulated peritoneal macrophages from C57/BL mice
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Positive control
- WB: Mouse spleen and macrophages lysate. ICC/IF: Raw264.7 cells; Mouse bone marrow cells. IHC-Fr: Mouse spleen and intestine tissue. Flow Cyt: J774 cells and peritoneal macrophages.
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General notes
Please note we cannot guarantee IHC-P, however should you use this application for this product then please use a specific protocol which can be found in the protocols section of our datasheet here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant. -
Clonality
Monoclonal -
Clone number
CI:A3-1 -
Myeloma
NS1 -
Isotype
IgG2b -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
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Related Products
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- Anti-F4/80 antibody [F4/80] - Macrophage Marker (ab90247)
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab6640 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| Application | Species |
|---|---|
| Flow Cyt |
Mouse
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| ICC/IF |
Mouse
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| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt |
1/10 - 1/50.
ab18536 - Rat monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
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| ICC/IF | (9) |
Use a concentration of 5 - 10 µg/ml.
We recommend ab150165 Goat Anti-Rat IgG H&L (Alexa Fluor® 488) secondary antibody. |
| Notes |
|---|
|
Flow Cyt
1/10 - 1/50. ab18536 - Rat monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
|
ICC/IF
Use a concentration of 5 - 10 µg/ml. We recommend ab150165 Goat Anti-Rat IgG H&L (Alexa Fluor® 488) secondary antibody. |
Target
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Function
Orphan receptor involved in cell adhesion and probably in cell-cell interactions specifically involving cells of the immune system. May play a role in regulatory T-cells (Treg) development. -
Tissue specificity
Expression is restricted to eosinophils. -
Sequence similarities
Belongs to the G-protein coupled receptor 2 family. Adhesion G-protein coupled receptor (ADGR) subfamily.
Contains 6 EGF-like domains.
Contains 1 GPS domain. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 13733 Mouse
- SwissProt: Q61549 Mouse
- Unigene: 2254 Mouse
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Alternative names
- ADGRE1 antibody
- Adhesion G protein coupled receptor E1 antibody
- Adhesion G protein-coupled receptor E1 antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence - Anti-F4/80 antibody [CI:A3-1] - Macrophage Marker (ab6640)
ab6640 stained Raw 264.7 cells. The cells were 100% methanol fixed for 5 minutes at room temperatureand then incubated in 1%BSA / 10% normal Goat serum / 0.3M glycine in 0.1% PBS-Tween for 1hour at room temperature to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab6640 at 5µg/ml) overnight at +4°C. The secondary antibody (pseudo-colored green) was ab150165 Goat Anti-Rat IgG H&L (Alexa Fluor® 488) preadsorbed used at a 1/1000 dilution for 1 hour at room temperature. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1hour at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.
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![Flow Cytometry - Anti-F4/80 antibody [CI:A3-1] - Macrophage Marker (ab6640)](https://www.abcam.com/ps/products/6/ab6640/Images/ab6640-264399-anti-f4-80-antibody-cia3-1-flow-cytometry.jpg)
Flow Cytometry - Anti-F4/80 antibody [CI:A3-1] - Macrophage Marker (ab6640)Flow cytometry analysis of J774 cells labelling F4/80 (red) with ab6640 at a dilution of 1/10 followed by streptavidin FITC secondary at 1/100 dilution. The blue line shows J774 cells stained with Rat anti mouse isotype control.
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![Immunocytochemistry/ Immunofluorescence - Anti-F4/80 antibody [CI:A3-1] - Macrophage Marker (ab6640)](https://www.abcam.com/ps/products/6/ab6640/Images/ab6640-12433-anti-f4-80-antibody-cia3-1-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-F4/80 antibody [CI:A3-1] - Macrophage Marker (ab6640)This image is courtesy of an anonymous Abreviewab6640 staining F4/80 in mouse bone marrow cells by immunocytochemistry/ immunofluorescence. Cells were formaldehyde fixed and permeabilized in 0.2% Triton X-100 prior to blocking in 2% BSA for 30 minutes at 20°C. The primary antibody was diluted 1/200 and incubated with the sample for 9 hour at 4°C. Alexa fluor® 488 goat polyclonal to rat Ig, diluted 1/200, was used as the secondary antibody. -
![Flow Cytometry - Anti-F4/80 antibody [CI:A3-1] - Macrophage Marker (ab6640)](https://www.abcam.com/ps/products/6/ab6640/Images/ab6640-264401-anti-f4-80-antibody-cia3-1-flow-cytometry.jpg)
Flow Cytometry - Anti-F4/80 antibody [CI:A3-1] - Macrophage Marker (ab6640)Flow cytometry analysis of peritoneal macrophages labelling F4/80 (red) with ab6640 at a dilution of 1/10. The blue line shows J774 cells stained with Rat anti mouse isotype control.
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![Flow Cytometry - Anti-F4/80 antibody [CI:A3-1] - Macrophage Marker (ab6640)](https://www.abcam.com/ps/products/6/ab6640/Images/ab6640-264400-anti-f4-80-antibody-cia3-1-flow-cytometry.jpg)
Flow Cytometry - Anti-F4/80 antibody [CI:A3-1] - Macrophage Marker (ab6640)Flow cytometry analysis of J774 cells labelling F4/80 (red) with ab6640 at a dilution of 1/50 followed by goat anti rat IgG FITC secondary antibody at 1/100 dilution. The blue line shows J774 cells stained with Rat anti mouse isotype control.
Protocols
References (603)
ab6640 has been referenced in 603 publications.
- Bi HL et al. Inhibition of UCHL1 by LDN-57444 attenuates Ang II-Induced atrial fibrillation in mice. Hypertens Res 43:168-177 (2020). PubMed: 31700166
- Wang C et al. Alpha-hemolysin of uropathogenic Escherichia coli induces GM-CSF-mediated acute kidney injury. Mucosal Immunol 13:22-33 (2020). PubMed: 31719643
- Wan Z et al. Mononuclear phagocyte system blockade improves therapeutic exosome delivery to the myocardium. Theranostics 10:218-230 (2020). PubMed: 31903116
- Tharp ME et al. Maximizing the ovarian reserve in mice by evading LINE-1 genotoxicity. Nat Commun 11:330 (2020). PubMed: 31949138
- Jones JEC et al. The Adipocyte Acquires a Fibroblast-Like Transcriptional Signature in Response to a High Fat Diet. Sci Rep 10:2380 (2020). PubMed: 32047213
