Anti-F4/80 antibody [CI:A3-1] - Macrophage Marker (ab6640)
![Immunocytochemistry/ Immunofluorescence - Anti-F4/80 antibody [CI:A3-1] - Macrophage Marker (ab6640)](https://www.abcam.com/ps/products/6/ab6640/Images/ab6640-266714-anti-f4-80-antibody-cia3-1-immunofluorescence.jpg "Immunocytochemistry/ Immunofluorescence - Anti-F4/80 antibody [CI:A3-1] - Macrophage Marker (ab6640)")
Key features and details
- Rat monoclonal [CI:A3-1] to F4/80 - Macrophage Marker
- Suitable for: Flow Cyt, ICC/IF
- Reacts with: Mouse
- Isotype: IgG2b
Related conjugates and formulations
Overview
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Product name
Anti-F4/80 antibody [CI:A3-1] - Macrophage Marker
See all F4/80 primary antibodies -
Description
Rat monoclonal [CI:A3-1] to F4/80 - Macrophage Marker -
Host species
Rat -
Specificity
Anti-F4/80 antibody (ab6640) recognises the mouse F4/80 antigen, a 160kD glycoprotein expressed by murine macrophages.
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Tested applications
Suitable for: Flow Cyt, ICC/IFmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
Tissue, cells or virus corresponding to F4/80. Thioglycollate stimulated peritoneal macrophages from C57/BL mice
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Positive control
- WB: Mouse spleen and macrophages lysate. ICC/IF: Raw264.7 cells; Mouse bone marrow cells. IHC-Fr: Mouse spleen and intestine tissue. Flow Cyt: J774 cells and peritoneal macrophages.
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General notes
Please note we cannot guarantee IHC-P, however should you use this application for this product then please use a specific protocol which can be found in the protocols section of our datasheet here.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant. -
Clonality
Monoclonal -
Clone number
CI:A3-1 -
Myeloma
NS1 -
Isotype
IgG2b -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
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Related Products
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- Anti-F4/80 antibody [F4/80] - Macrophage Marker (ab90247)
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab6640 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt |
1/10 - 1/50.
ab18536 - Rat monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
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| ICC/IF | (10) |
Use a concentration of 5 - 10 µg/ml.
We recommend ab150165 Goat Anti-Rat IgG H&L (Alexa Fluor® 488) secondary antibody. |
| Notes |
|---|
|
Flow Cyt
1/10 - 1/50. ab18536 - Rat monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
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ICC/IF
Use a concentration of 5 - 10 µg/ml. We recommend ab150165 Goat Anti-Rat IgG H&L (Alexa Fluor® 488) secondary antibody. |
Target
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Function
Orphan receptor involved in cell adhesion and probably in cell-cell interactions specifically involving cells of the immune system. May play a role in regulatory T-cells (Treg) development. -
Tissue specificity
Expression is restricted to eosinophils. -
Sequence similarities
Belongs to the G-protein coupled receptor 2 family. Adhesion G-protein coupled receptor (ADGR) subfamily.
Contains 6 EGF-like domains.
Contains 1 GPS domain. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 13733 Mouse
- SwissProt: Q61549 Mouse
- Unigene: 2254 Mouse
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Alternative names
- ADGRE1 antibody
- Adhesion G protein coupled receptor E1 antibody
- Adhesion G protein-coupled receptor E1 antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence - Anti-F4/80 antibody [CI:A3-1] - Macrophage Marker (ab6640)
ab6640 stained Raw 264.7 cells. The cells were 100% methanol fixed for 5 minutes at room temperatureand then incubated in 1%BSA / 10% normal Goat serum / 0.3M glycine in 0.1% PBS-Tween for 1hour at room temperature to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab6640 at 5µg/ml) overnight at +4°C. The secondary antibody (pseudo-colored green) was ab150165 Goat Anti-Rat IgG H&L (Alexa Fluor® 488) preadsorbed used at a 1/1000 dilution for 1 hour at room temperature. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1hour at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.
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![Flow Cytometry - Anti-F4/80 antibody [CI:A3-1] - Macrophage Marker (ab6640)](https://www.abcam.com/ps/products/6/ab6640/Images/ab6640-264399-anti-f4-80-antibody-cia3-1-flow-cytometry.jpg)
Flow Cytometry - Anti-F4/80 antibody [CI:A3-1] - Macrophage Marker (ab6640)Flow cytometry analysis of J774 cells labelling F4/80 (red) with ab6640 at a dilution of 1/10 followed by streptavidin FITC secondary at 1/100 dilution. The blue line shows J774 cells stained with Rat anti mouse isotype control.
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![Immunocytochemistry/ Immunofluorescence - Anti-F4/80 antibody [CI:A3-1] - Macrophage Marker (ab6640)](https://www.abcam.com/ps/products/6/ab6640/Images/ab6640-12433-anti-f4-80-antibody-cia3-1-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-F4/80 antibody [CI:A3-1] - Macrophage Marker (ab6640)This image is courtesy of an anonymous Abreviewab6640 staining F4/80 in mouse bone marrow cells by immunocytochemistry/ immunofluorescence. Cells were formaldehyde fixed and permeabilized in 0.2% Triton X-100 prior to blocking in 2% BSA for 30 minutes at 20°C. The primary antibody was diluted 1/200 and incubated with the sample for 9 hour at 4°C. Alexa fluor® 488 goat polyclonal to rat Ig, diluted 1/200, was used as the secondary antibody. -
![Flow Cytometry - Anti-F4/80 antibody [CI:A3-1] - Macrophage Marker (ab6640)](https://www.abcam.com/ps/products/6/ab6640/Images/ab6640-264401-anti-f4-80-antibody-cia3-1-flow-cytometry.jpg)
Flow Cytometry - Anti-F4/80 antibody [CI:A3-1] - Macrophage Marker (ab6640)Flow cytometry analysis of peritoneal macrophages labelling F4/80 (red) with ab6640 at a dilution of 1/10. The blue line shows J774 cells stained with Rat anti mouse isotype control.
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![Flow Cytometry - Anti-F4/80 antibody [CI:A3-1] - Macrophage Marker (ab6640)](https://www.abcam.com/ps/products/6/ab6640/Images/ab6640-264400-anti-f4-80-antibody-cia3-1-flow-cytometry.jpg)
Flow Cytometry - Anti-F4/80 antibody [CI:A3-1] - Macrophage Marker (ab6640)Flow cytometry analysis of J774 cells labelling F4/80 (red) with ab6640 at a dilution of 1/50 followed by goat anti rat IgG FITC secondary antibody at 1/100 dilution. The blue line shows J774 cells stained with Rat anti mouse isotype control.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (742)
ab6640 has been referenced in 742 publications.
- Caputo T et al. Anti-adipogenic signals at the onset of obesity-related inflammation in white adipose tissue. Cell Mol Life Sci 78:227-247 (2021). PubMed: 32157317
- Domínguez-Bautista JA et al. Programmed Cell Senescence in the Mouse Developing Spinal Cord and Notochord. Front Cell Dev Biol 9:587096 (2021). PubMed: 33575260
- Sun B et al. Hyperoside attenuates non-alcoholic fatty liver disease through targeting Nr4A1 in macrophages. Int Immunopharmacol 94:107438 (2021). PubMed: 33611063
- Hsieh TB et al. Deletion of Calponin 2 Reduces the Formation of Postoperative Peritoneal Adhesions. J Invest Surg N/A:1-12 (2021). PubMed: 33622156
- Li Y et al. Injectable hydrogel with MSNs/microRNA-21-5p delivery enables both immunomodification and enhanced angiogenesis for myocardial infarction therapy in pigs. Sci Adv 7:N/A (2021). PubMed: 33627421