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    f480-antibody-cia3-1-macrophage-marker-ab6640.pdf

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Immunology Innate Immunity Macrophage / Inflamm.
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Anti-F4/80 antibody [CI:A3-1] - Macrophage Marker (ab6640)

  • Datasheet
  • SDS
Reviews (66)Q&A (26)References (603)

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Immunocytochemistry/ Immunofluorescence - Anti-F4/80 antibody [CI:A3-1] - Macrophage Marker (ab6640)
  • Flow Cytometry - Anti-F4/80 antibody [CI:A3-1] - Macrophage Marker (ab6640)
  • Immunocytochemistry/ Immunofluorescence - Anti-F4/80 antibody [CI:A3-1] - Macrophage Marker (ab6640)
  • Flow Cytometry - Anti-F4/80 antibody [CI:A3-1] - Macrophage Marker (ab6640)
  • Flow Cytometry - Anti-F4/80 antibody [CI:A3-1] - Macrophage Marker (ab6640)

Key features and details

  • Rat monoclonal [CI:A3-1] to F4/80 - Macrophage Marker
  • Suitable for: Flow Cyt, ICC/IF
  • Reacts with: Mouse
  • Isotype: IgG2b

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Overview

  • Product name

    Anti-F4/80 antibody [CI:A3-1] - Macrophage Marker
    See all F4/80 primary antibodies
  • Description

    Rat monoclonal [CI:A3-1] to F4/80 - Macrophage Marker
  • Host species

    Rat
  • Specificity

    Anti-F4/80 antibody (ab6640) recognises the mouse F4/80 antigen, a 160kD glycoprotein expressed by murine macrophages. 

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Mouse
    ICC/IF
    Mouse
    See all applications and species data
  • Immunogen

    Thioglycollate stimulated peritoneal macrophages from C57/BL mice

  • Positive control

    • WB: Mouse spleen and macrophages lysate. ICC/IF: Raw264.7 cells; Mouse bone marrow cells. IHC-Fr: Mouse spleen and intestine tissue. Flow Cyt: J774 cells and peritoneal macrophages.
  • General notes

    Please note we cannot guarantee IHC-P, however should you use this application for this product then please use a specific protocol which can be found in the protocols section of our datasheet here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • Purity

    Protein G purified
  • Purification notes

    Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant.
  • Clonality

    Monoclonal
  • Clone number

    CI:A3-1
  • Myeloma

    NS1
  • Isotype

    IgG2b
  • Research areas

    • Immunology
    • Innate Immunity
    • Macrophage / Inflamm.
    • Tags & Cell Markers
    • Cell Type Markers
    • Other Cell Types
    • Signal Transduction
    • Antibodies
    • f4/80

Associated products

  • Alternative Versions

    • APC Anti-F4/80 antibody [CI:A3-1] (ab105080)
    • FITC Anti-F4/80 antibody [CI:A3-1] (ab105155)
    • PE Anti-F4/80 antibody [CI:A3-1] (ab105156)
  • Compatible Secondaries

    • Goat Anti-Rat IgG H&L (Alexa Fluor® 488) (ab150157)
    • Goat Anti-Rat IgG H&L (HRP) (ab205720)
  • Conjugation kits

    • PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918)
    • APC Conjugation Kit - Lightning-Link® (ab201807)
  • Isotype control

    • Rat IgG2b, kappa monoclonal [RTK4530] - Isotype control (ab18541)
  • Recombinant Protein

    • Recombinant Human EMR1/ADGRE1 protein (ab131740)
  • Related Products

    • Prestained Protein Ladder - Broad molecular weight (10-245 kDa) (ab116028)
    • Fix & Perm / Cell Fixation & Permeabilization Kit (Flow Cytometry) (ab185917)
    • Anti-TMEM119 antibody [28-3] - Microglial marker (ab209064)
    • Anti-CD68 antibody [FA-11] (ab53444)
    • Anti-CD11b antibody [M1/70] (ab8878)
    • Anti-F4/80 antibody [F4/80] - Macrophage Marker (ab90247)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab6640 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
Flow Cyt
Mouse
ICC/IF
Mouse
Application Abreviews Notes
Flow Cyt
1/10 - 1/50.

ab18536 - Rat monoclonal IgG2b, is suitable for use as an isotype control with this antibody.

ICC/IF (9)
Use a concentration of 5 - 10 µg/ml.

We recommend ab150165 Goat Anti-Rat IgG H&L (Alexa Fluor® 488) secondary antibody.

Notes
Flow Cyt
1/10 - 1/50.

ab18536 - Rat monoclonal IgG2b, is suitable for use as an isotype control with this antibody.

ICC/IF
Use a concentration of 5 - 10 µg/ml.

We recommend ab150165 Goat Anti-Rat IgG H&L (Alexa Fluor® 488) secondary antibody.

Target

  • Function

    Orphan receptor involved in cell adhesion and probably in cell-cell interactions specifically involving cells of the immune system. May play a role in regulatory T-cells (Treg) development.
  • Tissue specificity

    Expression is restricted to eosinophils.
  • Sequence similarities

    Belongs to the G-protein coupled receptor 2 family. Adhesion G-protein coupled receptor (ADGR) subfamily.
    Contains 6 EGF-like domains.
    Contains 1 GPS domain.
  • Cellular localization

    Cell membrane.
  • Target information above from: UniProt accession Q14246 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 13733 Mouse
    • SwissProt: Q61549 Mouse
    • Unigene: 2254 Mouse
    • Alternative names

      • ADGRE1 antibody
      • Adhesion G protein coupled receptor E1 antibody
      • Adhesion G protein-coupled receptor E1 antibody
      • AGRE1_HUMAN antibody
      • Cell surface glycoprotein EMR1 antibody
      • Cell surface glycoprotein F4/80 antibody
      • DD7A5 7 antibody
      • Egf like module containing mucin like hormone receptor like 1 antibody
      • Egf like module containing mucin like hormone receptor like sequence 1 antibody
      • EGF like module receptor 1 antibody
      • EGF TM7 antibody
      • EGF-like module receptor 1 antibody
      • EGF-like module-containing mucin-like hormone receptor-like 1 antibody
      • EGFTM7 antibody
      • EMR 1 antibody
      • EMR1 antibody
      • EMR1 hormone receptor antibody
      • Gpf480 antibody
      • Ly71 antibody
      • Lymphocyte antigen 71 antibody
      • TM7LN3 antibody
      see all

    Images

    • Immunocytochemistry/ Immunofluorescence - Anti-F4/80 antibody [CI:A3-1] - Macrophage Marker (ab6640)
      Immunocytochemistry/ Immunofluorescence - Anti-F4/80 antibody [CI:A3-1] - Macrophage Marker (ab6640)

      ab6640 stained Raw 264.7 cells. The cells were 100% methanol fixed for 5 minutes at room temperatureand then incubated in 1%BSA / 10% normal Goat serum / 0.3M glycine in 0.1% PBS-Tween for 1hour at room temperature to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab6640 at 5µg/ml) overnight at +4°C. The secondary antibody (pseudo-colored green) was ab150165 Goat Anti-Rat IgG H&L (Alexa Fluor® 488) preadsorbed used at a 1/1000 dilution for 1 hour at room temperature. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1hour at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.

    • Flow Cytometry - Anti-F4/80 antibody [CI:A3-1] - Macrophage Marker (ab6640)
      Flow Cytometry - Anti-F4/80 antibody [CI:A3-1] - Macrophage Marker (ab6640)

      Flow cytometry analysis of J774 cells labelling F4/80 (red) with ab6640 at a dilution of 1/10 followed by streptavidin FITC secondary at 1/100 dilution. The blue line shows J774 cells stained with Rat anti mouse isotype control. 

    • Immunocytochemistry/ Immunofluorescence - Anti-F4/80 antibody [CI:A3-1] - Macrophage Marker (ab6640)
      Immunocytochemistry/ Immunofluorescence - Anti-F4/80 antibody [CI:A3-1] - Macrophage Marker (ab6640)This image is courtesy of an anonymous Abreview
      ab6640 staining F4/80 in mouse bone marrow cells by immunocytochemistry/ immunofluorescence. Cells were formaldehyde fixed and permeabilized in 0.2% Triton X-100 prior to blocking in 2% BSA for 30 minutes at 20°C. The primary antibody was diluted 1/200 and incubated with the sample for 9 hour at 4°C. Alexa fluor® 488 goat polyclonal to rat Ig, diluted 1/200, was used as the secondary antibody.

      See Abreview

    • Flow Cytometry - Anti-F4/80 antibody [CI:A3-1] - Macrophage Marker (ab6640)
      Flow Cytometry - Anti-F4/80 antibody [CI:A3-1] - Macrophage Marker (ab6640)

      Flow cytometry analysis of peritoneal macrophages labelling F4/80 (red) with ab6640 at a dilution of 1/10. The blue line shows J774 cells stained with Rat anti mouse isotype control. 

    • Flow Cytometry - Anti-F4/80 antibody [CI:A3-1] - Macrophage Marker (ab6640)
      Flow Cytometry - Anti-F4/80 antibody [CI:A3-1] - Macrophage Marker (ab6640)

      Flow cytometry analysis of J774 cells labelling F4/80 (red) with ab6640 at a dilution of 1/50 followed by goat anti rat IgG FITC secondary antibody at 1/100 dilution. The blue line shows J774 cells stained with Rat anti mouse isotype control. 

    Protocols

    • Recommended IHC-P protocol with ab6640
    • Recommended FACS protocol for ab6640

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
    • SDS
  • References (603)

    Publishing research using ab6640? Please let us know so that we can cite the reference in this datasheet.

    ab6640 has been referenced in 603 publications.

    • Bi HL  et al. Inhibition of UCHL1 by LDN-57444 attenuates Ang II-Induced atrial fibrillation in mice. Hypertens Res 43:168-177 (2020). PubMed: 31700166
    • Wang C  et al. Alpha-hemolysin of uropathogenic Escherichia coli induces GM-CSF-mediated acute kidney injury. Mucosal Immunol 13:22-33 (2020). PubMed: 31719643
    • Wan Z  et al. Mononuclear phagocyte system blockade improves therapeutic exosome delivery to the myocardium. Theranostics 10:218-230 (2020). PubMed: 31903116
    • Tharp ME  et al. Maximizing the ovarian reserve in mice by evading LINE-1 genotoxicity. Nat Commun 11:330 (2020). PubMed: 31949138
    • Jones JEC  et al. The Adipocyte Acquires a Fibroblast-Like Transcriptional Signature in Response to a High Fat Diet. Sci Rep 10:2380 (2020). PubMed: 32047213
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review

    Filter by Application

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    1-10 of 66 Abreviews

    Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-F4/80 antibody [CI:A3-1] - Macrophage Marker

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (PFA perfusion fixed frozen sections)
    Sample
    Rat Tissue sections (Spleen)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citrate Buffer pH 6.0
    Permeabilization
    Yes - 0.05% Tween 20
    Specification
    Spleen
    Blocking step
    Sea Block as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 22°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Mar 03 2020

    Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-F4/80 antibody [CI:A3-1] - Macrophage Marker

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (PFA perfusion fixed frozen sections)
    Sample
    Mouse Tissue sections (Spleen)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citrate Buffer pH 6.0
    Permeabilization
    Yes - 0.05% Tween 20
    Specification
    Spleen
    Blocking step
    Sea Block as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 22°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Mar 03 2020

    IHC - Wholemount abreview for Anti-F4/80 antibody [CI:A3-1] - Macrophage Marker

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    IHC - Wholemount
    Sample
    Mouse Embryo (Kidney)
    Specification
    Kidney
    Read More

    Abcam user community

    Verified customer

    Submitted Feb 26 2020

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-F4/80 antibody [CI:A3-1] - Macrophage Marker

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Rat Tissue sections (Spleen)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citrate Buffer pH 6.0
    Permeabilization
    Yes - 0.05% Tween 20
    Specification
    Spleen
    Blocking step
    Sea Block as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 22°C
    Fixative
    Formaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Jan 15 2020

    Immunocytochemistry/ Immunofluorescence abreview for Anti-F4/80 antibody [CI:A3-1] - Macrophage Marker

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Mouse Cell (lung)
    Permeabilization
    No
    Specification
    lung
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Jan 08 2020

    Western blot abreview for Anti-F4/80 antibody [CI:A3-1] - Macrophage Marker

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (Liver)
    Gel Running Conditions
    Non-reduced Non-Denaturing (Native) (10)
    Loading amount
    30 µg
    Specification
    Liver
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    Submitted Jul 12 2019

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-F4/80 antibody [CI:A3-1] - Macrophage Marker

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (Brain (50um))
    Antigen retrieval step
    None
    Permeabilization
    Yes - 3 x 5mn PBS-T
    Specification
    Brain (50um)
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Jun 04 2019

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-F4/80 antibody [CI:A3-1] - Macrophage Marker

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (Upper jaw)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: 10x Citrate Buffer pH 6.0 (ab64214)
    Permeabilization
    No
    Specification
    Upper jaw
    Blocking step
    Protein block (X0909, DAKO) as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C
    Fixative
    Paraformaldehyde
    Read More

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    Verified customer

    Submitted Feb 28 2019

    Immunocytochemistry/ Immunofluorescence abreview for Anti-F4/80 antibody [CI:A3-1] - Macrophage Marker

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Mouse Cell (Bone marrow-derived macrophages)
    Permeabilization
    Yes - Triton X-100
    Specification
    Bone marrow-derived macrophages
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 22°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Sep 14 2017

    Immunohistochemistry (Frozen sections) abreview for Anti-F4/80 antibody [CI:A3-1] - Macrophage Marker

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue sections (Brain)
    Permeabilization
    Yes - 0.3% triton x100
    Specification
    Brain
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Mar 24 2017

    1-10 of 66 Abreviews

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