Anti-F4/80 antibody [CI:A3-1] (Phycoerythrin) (ab105156)


  • Product name
    Anti-F4/80 antibody [CI:A3-1] (Phycoerythrin)
    See all F4/80 primary antibodies
  • Description
    Rat monoclonal [CI:A3-1] to F4/80 (Phycoerythrin)
  • Host species
  • Conjugation
    Phycoerythrin. Ex: 488nm, Em: 575nm
  • Specificity
    ab105156 recognises the murine F4/80 antigen.
  • Tested applications
    Suitable for: Flow Cyt, IHC-Fr, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    Thioglycollate stimulated peritoneal cavity macrophages from C57/BL mice.

  • Positive control
    • Mouse J774 cells
  • General notes

    Clone CI:A3-1 has been reported to modulate cytokine levels released in response to Listeria monocytogenes.


  • Form
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Storage buffer
    pH: 7.40
    Preservative: 0.09% Sodium azide
    Constituents: 1% BSA, PBS, 5% Sucrose
  • Concentration information loading...
  • Purity
    Protein A purified
  • Primary antibody notes
    Clone CI:A3-1 has been reported to modulate cytokine levels released in response to Listeria monocytogenes.
  • Clonality
  • Clone number
  • Myeloma
  • Isotype
  • Research areas


Our Abpromise guarantee covers the use of ab105156 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 10µl for 106 cells.

in 100 µl.

ab136585 - Rat monoclonal IgG2b, is suitable for use as an isotype control with this antibody.

IHC-Fr Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.


  • Function
    Orphan receptor involved in cell adhesion and probably in cell-cell interactions specifically involving cells of the immune system. May play a role in regulatory T-cells (Treg) development.
  • Tissue specificity
    Expression is restricted to eosinophils.
  • Sequence similarities
    Belongs to the G-protein coupled receptor 2 family. Adhesion G-protein coupled receptor (ADGR) subfamily.
    Contains 6 EGF-like domains.
    Contains 1 GPS domain.
  • Cellular localization
    Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • ADGRE1 antibody
    • Adhesion G protein coupled receptor E1 antibody
    • Adhesion G protein-coupled receptor E1 antibody
    • AGRE1_HUMAN antibody
    • Cell surface glycoprotein EMR1 antibody
    • Cell surface glycoprotein F4/80 antibody
    • DD7A5 7 antibody
    • Egf like module containing mucin like hormone receptor like 1 antibody
    • Egf like module containing mucin like hormone receptor like sequence 1 antibody
    • EGF like module receptor 1 antibody
    • EGF TM7 antibody
    • EGF-like module receptor 1 antibody
    • EGF-like module-containing mucin-like hormone receptor-like 1 antibody
    • EGFTM7 antibody
    • EMR 1 antibody
    • EMR1 hormone receptor antibody
    • Gpf480 antibody
    • Ly71 antibody
    • Lymphocyte antigen 71 antibody
    • TM7LN3 antibody
    see all


  • Flow cytometry staining of mouse J774 cells with ab105156.


This product has been referenced in:
  • Li H  et al. Fibroblast growth factor 21 increases insulin sensitivity through specific expansion of subcutaneous fat. Nat Commun 9:272 (2018). Read more (PubMed: 29348470) »
  • Neng L  et al. Isolation and culture of endothelial cells, pericytes and perivascular resident macrophage-like melanocytes from the young mouse ear. Nat Protoc 8:709-20 (2013). Read more (PubMed: 23493068) »

See all 2 Publications for this product

Customer reviews and Q&As

Vielen Dank für Ihre Anfrage.

Leider ist es nicht möglich die Konzentration von ab105156 zu bestimmen.

Bei ab105156 handelt es sich einen konjugierten Antikörper.

Die Konzentration von Antikörpern wird ...

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Thank you for your inquiry.

I can confirm that the volume of ab105156 is 500ul per vial. The concentration cannot be determined since this a PE-conjugated antibody. An estimatewould be ˜ 0.1mg/ml.

I suggest to use two concentrations...

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Thank you for your query. I can confirm that the unconjugated clone of ab105156 was typically used at around 10ug/ml for frozen sections. However, I would still recommend to titrate the antibody in order to determine the optimal concentration f...

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Thank you for your interest. Just to confirm ab105156 is a primary antibody which is conjugated to PE. This means that it is not necessary to apply a compatible secondary antibody for detection. For further details (tested applications,...

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Thank you for your inquiry. We have a number of antibodies in our catalog that might be of interest to you. I would like to direct you to the search facility on our website ( Please enter the name of the protein into the search box a...

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