Overview

  • Product name
    Anti-F4/80 antibody [CI:A3-1] (Phycoerythrin)
    See all F4/80 primary antibodies
  • Description
    Rat monoclonal [CI:A3-1] to F4/80 (Phycoerythrin)
  • Host species
    Rat
  • Conjugation
    Phycoerythrin. Ex: 488nm, Em: 575nm
  • Specificity
    ab105156 recognises the murine F4/80 antigen.
  • Tested applications
    Suitable for: Flow Cyt, IHC-Fr, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    Thioglycollate stimulated peritoneal cavity macrophages from C57/BL mice.

  • Positive control
    • Mouse J774 cells
  • General notes


    Clone CI:A3-1 has been reported to modulate cytokine levels released in response to Listeria monocytogenes.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Storage buffer
    pH: 7.40
    Preservative: 0.09% Sodium azide
    Constituents: 1% BSA, PBS, 5% Sucrose
  • Concentration information loading...
  • Purity
    Protein A purified
  • Primary antibody notes
    Clone CI:A3-1 has been reported to modulate cytokine levels released in response to Listeria monocytogenes.
  • Clonality
    Monoclonal
  • Clone number
    CI:A3-1
  • Myeloma
    NS1
  • Isotype
    IgG2b
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab105156 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 10µl for 106 cells.

in 100 µl.

ab136585 - Rat monoclonal IgG2b, is suitable for use as an isotype control with this antibody.

IHC-Fr Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.

Target

  • Function
    Orphan receptor involved in cell adhesion and probably in cell-cell interactions specifically involving cells of the immune system. May play a role in regulatory T-cells (Treg) development.
  • Tissue specificity
    Expression is restricted to eosinophils.
  • Sequence similarities
    Belongs to the G-protein coupled receptor 2 family. Adhesion G-protein coupled receptor (ADGR) subfamily.
    Contains 6 EGF-like domains.
    Contains 1 GPS domain.
  • Cellular localization
    Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • ADGRE1 antibody
    • Adhesion G protein coupled receptor E1 antibody
    • Adhesion G protein-coupled receptor E1 antibody
    • AGRE1_HUMAN antibody
    • Cell surface glycoprotein EMR1 antibody
    • Cell surface glycoprotein F4/80 antibody
    • DD7A5 7 antibody
    • Egf like module containing mucin like hormone receptor like 1 antibody
    • Egf like module containing mucin like hormone receptor like sequence 1 antibody
    • EGF like module receptor 1 antibody
    • EGF TM7 antibody
    • EGF-like module receptor 1 antibody
    • EGF-like module-containing mucin-like hormone receptor-like 1 antibody
    • EGFTM7 antibody
    • EMR 1 antibody
    • EMR1 hormone receptor antibody
    • Gpf480 antibody
    • Ly71 antibody
    • Lymphocyte antigen 71 antibody
    • TM7LN3 antibody
    see all

Images

  • Flow cytometry staining of mouse J774 cells with ab105156.

References

This product has been referenced in:
  • Li H  et al. Fibroblast growth factor 21 increases insulin sensitivity through specific expansion of subcutaneous fat. Nat Commun 9:272 (2018). Read more (PubMed: 29348470) »
  • Neng L  et al. Isolation and culture of endothelial cells, pericytes and perivascular resident macrophage-like melanocytes from the young mouse ear. Nat Protoc 8:709-20 (2013). Read more (PubMed: 23493068) »
See all 2 Publications for this product

Customer reviews and Q&As

1-5 of 5 Abreviews or Q&A

Answer

Vielen Dank für Ihre Anfrage.

Leider ist es nicht möglich die Konzentration von ab105156 zu bestimmen.

Bei ab105156 handelt es sich einen konjugierten Antikörper.

Die Konzentration von Antikörpern wird über die Proteinmenge bestimmt. ab105156 enthält aber nicht nur den spezifischen Antikörper sondern auch Phycoerythrin und macht deshalb eine Konzentrationsbestimmung nach der Konjugationunmöglich.

Wir empfehlen als Anhaltspunkt eine Konzentration von 0.1 mg/ml anzunehmen.

Es tut mir leid, dass ich Ihnen keine genaueren Angaben machen konnte und hoffe diese Information ist dennoch hilfreich.

Ich wünsche Ihnen viel Erfolg mit Ihrer Forschung und verbleibe

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Answer

Thank you for your inquiry.

I can confirm that the volume of ab105156 is 500ul per vial. The concentration cannot be determined since this a PE-conjugated antibody. An estimatewould be ˜ 0.1mg/ml.

I suggest to use two concentrations for the first experiment to get results that will be meaningful for further optimization. I recommend 1:50 and and 1:500. Optimal concentrations always have to be determined by the end user.

I am sorry I could not provide a more exact answer to your question on this occasion and hope this information is nevertheless helpful to your customer.

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Answer

Thank you for your query. I can confirm that the unconjugated clone of ab105156 was typically used at around 10ug/ml for frozen sections. However, I would still recommend to titrate the antibody in order to determine the optimal concentration for your specific experimental requirements. Although we have not specifically measured the concentration of the antibody after conjugation, based on our records, we would expect the concentration of this product to be approximately 0.1mg/ml. 50 test means this antibody can be used for 50 tests in flow cytometry when used at 10µl for 10 x 6 cells (in 100 µl.). I hope this information is helpful. Please do not hesitate to contact me again if you have any further questions.

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Answer

Thank you for your interest. Just to confirm ab105156 is a primary antibody which is conjugated to PE. This means that it is not necessary to apply a compatible secondary antibody for detection. For further details (tested applications, purification, unit size etc) please take a look at the on-line datasheet: https://www.abcam.com/f4-80-antibody-ci-a3-1-phycoerythrin-ab105156.html If you need any further assistance in the future, please do not hesitate to cont

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Answer

Thank you for your inquiry. We have a number of antibodies in our catalog that might be of interest to you. I would like to direct you to the search facility on our website (www.abcam.com). Please enter the name of the protein into the search box at the top of the home page. A list of products will be generated which can be further refined using the filters on the left hand side of the page. Using these filters will help you to identify products that have been tested in the applications and species you are interested in. I have conducted a search for you and found one antibody which may be interest to you: ab105156 - Rat monoclonal [CI:A3-1] to F4/80 (Phycoerythrin) - it recognizes mouse F4/80 antigen. I hope this information is helpful to you. If you have specific questions about the products identified during the search, please do not hesitate to contact me.  

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