Recombinant
RabMAb

Recombinant Anti-F4/80 antibody [EPR20548] (Alexa Fluor® 647) (ab237332)

Overview

  • Product name

    Anti-F4/80 antibody [EPR20548] (Alexa Fluor® 647)
    See all F4/80 primary antibodies
  • Description

    Rabbit monoclonal [EPR20548] to F4/80 (Alexa Fluor® 647)
  • Host species

    Rabbit
  • Conjugation

    Alexa Fluor® 647. Ex: 652nm, Em: 668nm
  • Tested applications

    Suitable for: Flow Cytmore details
  • Species reactivity

    Reacts with: Mouse
  • Immunogen

    Synthetic peptide within Mouse F4/80 aa 350-650. The exact sequence is proprietary.
    Database link: Q61549

  • Positive control

    • Flow Cyt: C57BL/6 mouse splenocytes.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: 30% Glycerol, 1% BSA, PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR20548
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab237332 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/2500.

Target

  • Function

    Orphan receptor involved in cell adhesion and probably in cell-cell interactions specifically involving cells of the immune system. May play a role in regulatory T-cells (Treg) development.
  • Tissue specificity

    Expression is restricted to eosinophils.
  • Sequence similarities

    Belongs to the G-protein coupled receptor 2 family. Adhesion G-protein coupled receptor (ADGR) subfamily.
    Contains 6 EGF-like domains.
    Contains 1 GPS domain.
  • Cellular localization

    Cell membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • ADGRE1 antibody
    • Adhesion G protein coupled receptor E1 antibody
    • Adhesion G protein-coupled receptor E1 antibody
    • AGRE1_HUMAN antibody
    • Cell surface glycoprotein EMR1 antibody
    • Cell surface glycoprotein F4/80 antibody
    • DD7A5 7 antibody
    • Egf like module containing mucin like hormone receptor like 1 antibody
    • Egf like module containing mucin like hormone receptor like sequence 1 antibody
    • EGF like module receptor 1 antibody
    • EGF TM7 antibody
    • EGF-like module receptor 1 antibody
    • EGF-like module-containing mucin-like hormone receptor-like 1 antibody
    • EGFTM7 antibody
    • EMR 1 antibody
    • EMR1 antibody
    • EMR1 hormone receptor antibody
    • Gpf480 antibody
    • Ly71 antibody
    • Lymphocyte antigen 71 antibody
    • TM7LN3 antibody
    see all

Images

  • C57BL/6 mouse splenocytes stained with ab237332 (right) or Rabbit IgG (monoclonal) Alexa Fluor® 647 (ab199093) isotype (left). C57BL/6 mouse splenocytes were incubated for 30 min on ice in 1x PBS containing 10 µg/ml anti CD16/CD32 and 10 % normal goat serum to block FC receptors and non-specific protein-protein interaction followed by the antibody (ab237332) or Rabbit IgG (monoclonal) Alexa Fluor® 647 (ab199093) isotype (1x 106 in 100 µl at 0.2 µg/ml (1/2500)) for 30 min on ice. The cells were simultaneously stained with CD11b.

    Acquisition of >30,000 events were collected using a 40 mW Red laser (640nm) and 670/14 bandpass filter. Events were gated on viable CD19 and CD3 negative lymphocytes.

References

ab237332 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab237332.
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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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