Recombinant Anti-F4/80 antibody [EPR20548] - BSA and Azide free (ab234530)
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Overview
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Product name
Anti-F4/80 antibody [EPR20548] - BSA and Azide free
See all F4/80 primary antibodies -
Description
Rabbit monoclonal [EPR20548] to F4/80 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
Synthetic peptide within Mouse F4/80 aa 350-650. The exact sequence is proprietary.
Database link: Q61549 -
Positive control
- Flow Cyt: Mouse primary bone marrow cells.
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General notes
Ab234530 is the carrier-free version of ab213200. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
ab234530 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Constituent: PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR20548 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab234530 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt | 1/500. |
Target
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Function
Orphan receptor involved in cell adhesion and probably in cell-cell interactions specifically involving cells of the immune system. May play a role in regulatory T-cells (Treg) development. -
Tissue specificity
Expression is restricted to eosinophils. -
Sequence similarities
Belongs to the G-protein coupled receptor 2 family. Adhesion G-protein coupled receptor (ADGR) subfamily.
Contains 6 EGF-like domains.
Contains 1 GPS domain. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 13733 Mouse
- SwissProt: Q61549 Mouse
- Unigene: 2254 Mouse
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Alternative names
- ADGRE1 antibody
- Adhesion G protein coupled receptor E1 antibody
- Adhesion G protein-coupled receptor E1 antibody
see all
Images
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![Flow Cytometry - Anti-F4/80 antibody [EPR20548] - BSA and Azide free (ab234530)](https://www.abcam.com/ps/products/234/ab234530/Images/ab234530-308580-anti-f4-80-antibody-epr20548-flow-cytometry.jpg)
Flow Cytometry - Anti-F4/80 antibody [EPR20548] - BSA and Azide free (ab234530)Flow cytometric analysis of Mouse primary bone marrow cells labeling F4/80 with ab213200 at 1/500 dilution (right panel) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (left panel). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/2000 dilution was used as the secondary antibody.
The expression profile is consistent with the literature. CD11b+/ F4/80+ cells represent a rare population of macrophages in mouse bone marrow (PMID: 19620305 and 20625428).
Gated on viable cells.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab213200).
Datasheets and documents
References
ab234530 has not yet been referenced specifically in any publications.
