Key features and details
- Rat monoclonal [F4/80] to F4/80 - Macrophage Marker
- Suitable for: ICC/IF
- Reacts with: Mouse
- Isotype: IgG2b
Related conjugates and formulations
Product nameAnti-F4/80 antibody [F4/80] - Macrophage Marker
See all F4/80 primary antibodies
DescriptionRat monoclonal [F4/80] to F4/80 - Macrophage Marker
Tested applicationsSuitable for: ICC/IFmore details
Species reactivityReacts with: Mouse
Tissue, cells or virus. This information is proprietary to Abcam and/or its suppliers.
- ICC/IF: Raw 264.7 cells.
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact email@example.com.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 6.97% L-Arginine
Concentration information loading...
PurityProtein G purified
Light chain typekappa
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab90247 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 5 - 10 µg/ml.
Use a concentration of 5 - 10 µg/ml.
FunctionOrphan receptor involved in cell adhesion and probably in cell-cell interactions specifically involving cells of the immune system. May play a role in regulatory T-cells (Treg) development.
Tissue specificityExpression is restricted to eosinophils.
Sequence similaritiesBelongs to the G-protein coupled receptor 2 family. Adhesion G-protein coupled receptor (ADGR) subfamily.
Contains 6 EGF-like domains.
Contains 1 GPS domain.
Cellular localizationCell membrane.
- Information by UniProt
- ADGRE1 antibody
- Adhesion G protein coupled receptor E1 antibody
- Adhesion G protein-coupled receptor E1 antibody
ab90247 stained Raw 264.7 cells. The cells were 100% methanol fixed (5 minutes) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Triton for 1hour at room temperature to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab90247 at 10µg/ml) overnight at +4°C. The secondary antibody (pseudo-colored green) was Alexa Fluor® 488 goat anti-rat IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1hour at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.
ab90247 has been referenced in 28 publications.
- Zhang S et al. Construction and Optimization of an Endometrial Injury Model in Mice by Transcervical Ethanol Perfusion. Reprod Sci 28:693-702 (2021). PubMed: 32939736
- Jiang D et al. Injury triggers fascia fibroblast collective cell migration to drive scar formation through N-cadherin. Nat Commun 11:5653 (2020). PubMed: 33159076
- Rahman FA et al. Impaired ECM Remodeling and Macrophage Activity Define Necrosis and Regeneration Following Damage in Aged Skeletal Muscle. Int J Mol Sci 21:N/A (2020). PubMed: 32605082
- Sun G et al. Molybdenum disulfide nanoflowers mediated anti-inflammation macrophage modulation for spinal cord injury treatment. J Colloid Interface Sci 549:50-62 (2019). PubMed: 31015056
- Zhou LN et al. Macrophage polarization in human gingival tissue in response to periodontal disease. Oral Dis 25:265-273 (2019). PubMed: 30285304