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  1. Link

    f480-antibody-sp115-ab111101.pdf

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Immunology Innate Immunity Macrophage / Inflamm.
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RecombinantRabMAb

Recombinant Anti-F4/80 antibody [SP115] (ab111101)

  • Datasheet
  • SDS
Reviews (20)Q&A (8)References (56)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-F4/80 antibody [SP115] (ab111101)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-F4/80 antibody [SP115] (ab111101)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-F4/80 antibody [SP115] (ab111101)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-F4/80 antibody [SP115] (ab111101)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-F4/80 antibody [SP115] (ab111101)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-F4/80 antibody [SP115] (ab111101)
  • Anti-F4/80 antibody [SP115] (ab111101)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [SP115] to F4/80
  • Suitable for: IHC-P
  • Reacts with: Mouse

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Protein
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Recombinant Human EMR1/ADGRE1 protein (ab131740)
Secondary
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Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

View more associated products

Overview

  • Product name

    Anti-F4/80 antibody [SP115]
    See all F4/80 primary antibodies
  • Description

    Rabbit monoclonal [SP115] to F4/80
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-Pmore details
    Unsuitable for: Flow Cyt
  • Species reactivity

    Reacts with: Mouse
  • Immunogen

    Synthetic peptide within Mouse F4/80 aa 50-150 (N terminal). The exact sequence is proprietary.
    Database link: Q61549

  • Positive control

    • IHC-P: Mouse colon, liver and lung tissue; M1 and M2 macrophages from mice colon tissue.
  • General notes

    FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 7.20
    Preservative: 0.1% Sodium azide
    Constituents: 1% BSA, PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Purification notes

    Purified from TCS by protein A/G.
  • Clonality

    Monoclonal
  • Clone number

    SP115
  • Isotype

    IgG
  • Research areas

    • Immunology
    • Innate Immunity
    • Macrophage / Inflamm.
    • Tags & Cell Markers
    • Cell Type Markers
    • Tumor Associated
    • Tags & Cell Markers
    • Cell Type Markers
    • Other Cell Types
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Cancer susceptibility
    • Proto-oncogenes
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Transcription Factors
    • Cancer
    • Oncoproteins/suppressors
    • Oncoproteins
    • Transcription factors

Associated products

  • Alternative Versions

    • Anti-F4/80 antibody [SP115] - BSA and Azide free (ab240946)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Recombinant Protein

    • Recombinant Human EMR1/ADGRE1 protein (ab131740)

Applications

Our Abpromise guarantee covers the use of ab111101 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/100.

Incubate primary antibody overnight at 4C.  For antigen retrieval: Boil tissue section in Tris-EDTA (pH 9.0) buffer for 10 min followed by cooling at RT for 20 min. Abcam recommends using a polymer-HRP conjugated secondary for optimal signal.

  • Application notes
    Is unsuitable for Flow Cyt.
  • Target

    • Function

      Orphan receptor involved in cell adhesion and probably in cell-cell interactions specifically involving cells of the immune system. May play a role in regulatory T-cells (Treg) development.
    • Tissue specificity

      Expression is restricted to eosinophils.
    • Sequence similarities

      Belongs to the G-protein coupled receptor 2 family. Adhesion G-protein coupled receptor (ADGR) subfamily.
      Contains 6 EGF-like domains.
      Contains 1 GPS domain.
    • Cellular localization

      Cell membrane.
    • Target information above from: UniProt accession Q14246 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 13733 Mouse
      • SwissProt: Q61549 Mouse
      • Unigene: 2254 Mouse
      • Alternative names

        • ADGRE1 antibody
        • Adhesion G protein coupled receptor E1 antibody
        • Adhesion G protein-coupled receptor E1 antibody
        • AGRE1_HUMAN antibody
        • Cell surface glycoprotein EMR1 antibody
        • Cell surface glycoprotein F4/80 antibody
        • DD7A5 7 antibody
        • Egf like module containing mucin like hormone receptor like 1 antibody
        • Egf like module containing mucin like hormone receptor like sequence 1 antibody
        • EGF like module receptor 1 antibody
        • EGF TM7 antibody
        • EGF-like module receptor 1 antibody
        • EGF-like module-containing mucin-like hormone receptor-like 1 antibody
        • EGFTM7 antibody
        • EMR 1 antibody
        • EMR1 antibody
        • EMR1 hormone receptor antibody
        • Gpf480 antibody
        • Ly71 antibody
        • Lymphocyte antigen 71 antibody
        • TM7LN3 antibody
        see all

      Images

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-F4/80 antibody [SP115] (ab111101)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-F4/80 antibody [SP115] (ab111101)

        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Mouse liver tissue sections labeling F4/80 with ab111101 at 1/250 dilution (0.48 µg/ml). Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). Goat Anti-Rabbit IgG H&L (HRP) was used as the secondary antibody. Hematoxylin was used as a counterstain. Positive staining on macrophages in the mouse liver.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-F4/80 antibody [SP115] (ab111101)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-F4/80 antibody [SP115] (ab111101)Lean, Q.Y. et al PLoS One. 2015 Jul 28;10(7):e0134259. doi: 10.1371/journal.pone.0134259. eCollection 2015 Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

        Representative immunostaining of F4/80-positive macrophages in the distal colon from healthy and colitic mice treated with and without enoxaparin.

        For immunohistochemical staining, antigen retrieval was performed by incubating the sections for 10 minutes at 97°C in 1 mM EDTA buffer, pH 8 or 10 mM citrate buffer, pH 6. Activity of endogenous peroxidase was blocked by incubating sections with 3% v/v hydrogen for 20 minutes. Sections were then washed with 0.05 M Tris-buffered saline containing 0.5% v/v Tween 20 (TBST), pH 7.6. Subsequently, sections were incubated with serum-free protein block for 10 minutes. Colon sections were then incubated with primary antibody ab111101 at 1/100 dilution overnight at 4°C or room temperature for 1 hour. Sections were then washed 3 x 5 minutes and allowed to react with secondary antibody: anti-rabbit immunoglobulin C conjugated to horseradish peroxidase (HRP) (ab7090) at 1/300 dilution at room temperature for 1 hour.

        Scale bar = 100 μm for 400 x magnification. Control, C; untreated colitis, DSS; oral enoxaparin, OE; intraperitoneal injection of enoxaparin, IPE.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-F4/80 antibody [SP115] (ab111101)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-F4/80 antibody [SP115] (ab111101)Lean, Q.Y. et al PLoS One. 2015 Jul 28;10(7):e0134259. doi: 10.1371/journal.pone.0134259. eCollection 2015 Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

        Representative images of (A) M1 macrophages (F4/80+ and iNOS+) and (B) M2 macrophages (F4/80+ and CD206+) using colon tissue from n = 3–5 mice. F4/80 positive cells were visualized using Alexa Fluor 594-conjugated goat anti-rat IgG (red). Nuclei were stained with 4’,6-diamidino-2-phenylindole (DAPI, blue).

        Scale bar = 50 μm for 400 × magnification. Control, C; untreated colitis, DSS; colitis with oral enoxaparin, DSS+OE.

        For immunofluorescence staining, sections were dewaxed and rehydrated before antigen retrieval using 10 mM citrate buffer, pH 6 for 15 minutes at 97°C. Sections were incubated with serum-free protein block and permeabilized with 0.4% v/v Triton-X at room temperature for 30 minutes. Sections were incubated with primary antibodies anti-F4/80 (ab16911) at 1/25 dilution overnight at 4°C or at room temperature for 1 hour. Sections were washed with TBST 3 × 10 minutes and incubated with species-specific secondary antibodies: anti-rat IgG H&L AlexaFluor 594 (ab150160, Abcam, 1:1000) and anti-rabbit IgG H&L AlexaFluor 488 (A11070{"type":"entrez-nucleotide","attrs":{"text":"A11070","term_id":"490922","term_text":"A11070"}}, Thermo Fisher Scientific, Melbourne, Australia, 1:1000) at room temperature for 2 hours. Sections were rinsed with TBST 3 × 10 minutes, followed by a quick wash with distilled water before mounting using Glycerol Mounting Medium (Abcam) that contained 4’,6-diamidino-2-phenylindole (DAPI) and 1,4-diazobicyclo-2,2,2-octane (DABCO). Labelled tissues were visualized using a Leica DM LB2 microscope. Fluorescence images (400 × magnification) were captured using NIS-Elements 4.13 (Nikon) software. 

        For full image see PMID: 26218284.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-F4/80 antibody [SP115] (ab111101)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-F4/80 antibody [SP115] (ab111101)

        ab111101 at 1/100 dilution staining F4/80 in Formalin-fixed, paraffin-embedded Mouse liver tissue.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-F4/80 antibody [SP115] (ab111101)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-F4/80 antibody [SP115] (ab111101)This image is of an Abreciew submitted by Francois Daubeuf.
        Immunohistochemistry analysis of Formalin fixed paraffin-embedded mouse lung tissue sections labeling F4/80 with ab111101 at 1/200 for 16 hours at 4°C. Biotin conjugated Goat anti-rabbit polyclonal antibody at 1/500 was used as the secondary. Antigen retrieval was heat mediated using citrate buffer pH 6.0. 

        See Abreview

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-F4/80 antibody [SP115] (ab111101)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-F4/80 antibody [SP115] (ab111101)

        Immunohistochemical analysis staining for macrophages in (A) mouse uterus and (B) mouse spleen using ab111101 at a dilution of 1:200. HRP Anti-Rabbit IgG (Peroxidase) Polymer D antibody was used as a secondary. 

        See Abreview

      • Anti-F4/80 antibody [SP115] (ab111101)
        Anti-F4/80 antibody [SP115] (ab111101)

      Protocols

      • Immunohistochemistry protocols

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet
      • SDS
    • References (56)

      Publishing research using ab111101? Please let us know so that we can cite the reference in this datasheet.

      ab111101 has been referenced in 56 publications.

      • Luchtel RA  et al. High-dose ascorbic acid synergizes with anti-PD1 in a lymphoma mouse model. Proc Natl Acad Sci U S A 117:1666-1677 (2020). PubMed: 31911474
      • Wang B  et al. The inhibitor of autophagy SBI-0206965 aggravates atherosclerosis through decreasing myeloid-derived suppressor cells. Exp Ther Med 19:1370-1378 (2020). PubMed: 32010311
      • von Roemeling CA  et al. Therapeutic modulation of phagocytosis in glioblastoma can activate both innate and adaptive antitumour immunity. Nat Commun 11:1508 (2020). PubMed: 32198351
      • Nagano N  et al. Attenuation of Hyperoxic Lung Injury in Newborn Thioredoxin-1-Overexpressing Mice through the Suppression of Proinflammatory Cytokine mRNA Expression. Biomedicines 8:N/A (2020). PubMed: 32244938
      • Wu L  et al. Targeting DUSP7 signaling alleviates hepatic steatosis, inflammation and oxidative stress in high fat diet (HFD)-fed mice via suppression of TAK1. Free Radic Biol Med 153:140-158 (2020). PubMed: 32311490
      View all Publications for this product

      Customer reviews and Q&As

      Show All Reviews Q&A
      Submit a review Submit a question

      1-10 of 28 Abreviews or Q&A

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-F4/80 antibody [SP115]

      Average
      Abreviews
      Abreviews
      abreview image
      Application
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
      Sample
      Mouse Tissue sections (Liver, macrophages)
      Antigen retrieval step
      Heat mediated - Buffer/Enzyme Used: Tris-EDTA pH9
      Permeabilization
      No
      Specification
      Liver, macrophages
      Blocking step
      Serum as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 4°C
      Fixative
      10% NBF
      Read More

      Vicki Shone

      Verified customer

      Submitted Nov 16 2020

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-F4/80 antibody [SP115]

      Excellent
      Abreviews
      Abreviews
      abreview image
      Application
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
      Sample
      Mouse Tissue sections (Mammary Glands)
      Antigen retrieval step
      Heat mediated - Buffer/Enzyme Used: Citrate Buffer
      Permeabilization
      No
      Specification
      Mammary Glands
      Blocking step
      Protein Blocking Solution as blocking agent for 10 minute(s) · Concentration: 100% · Temperature: 25°C
      Fixative
      Formaldehyde
      Read More

      Abcam user community

      Verified customer

      Submitted Aug 25 2020

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-F4/80 antibody [SP115]

      Average
      Abreviews
      Abreviews
      abreview image
      Application
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
      Sample
      Mouse Tissue sections (Uterus)
      Antigen retrieval step
      Heat mediated - Buffer/Enzyme Used: Sodium citrate
      Permeabilization
      Yes - Tween-20
      Specification
      Uterus
      Blocking step
      Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
      Fixative
      Paraformaldehyde
      Read More

      Reshef Tal

      Verified customer

      Submitted Sep 05 2019

      Immunohistochemistry (Frozen sections) abreview for Anti-F4/80 antibody [SP115]

      Average
      Abreviews
      Abreviews
      abreview image
      Application
      Immunohistochemistry (Frozen sections)
      Sample
      Mouse Tissue sections (Bone)
      Permeabilization
      Yes - 0.05% Triton-100X
      Specification
      Bone
      Blocking step
      Vetor Blocker as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 23°C
      Fixative
      Paraformaldehyde
      Read More

      Dr. Hanson Lee

      Verified customer

      Submitted Aug 19 2019

      Immunohistochemistry (Frozen sections) abreview for Anti-F4/80 antibody [SP115]

      Excellent
      Abreviews
      Abreviews
      abreview image
      Application
      Immunohistochemistry (Frozen sections)
      Sample
      Mouse Tissue sections (Liver)
      Permeabilization
      No
      Specification
      Liver
      Blocking step
      BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 22°C
      Fixative
      Paraformaldehyde
      Read More

      Abcam user community

      Verified customer

      Submitted Jun 13 2019

      Immunohistochemistry (Frozen sections) abreview for Anti-F4/80 antibody [SP115]

      Inconclusive
      Abreviews
      Abreviews
      abreview image
      Application
      Immunohistochemistry (Frozen sections)
      Sample
      Mouse Tissue sections (Mouse spleen)
      Permeabilization
      No
      Specification
      Mouse spleen
      Blocking step
      BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: rt°C
      Fixative
      Acetone
      Read More

      Abcam user community

      Verified customer

      Submitted Feb 28 2018

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-F4/80 antibody [SP115]

      Good
      Abreviews
      Abreviews
      abreview image
      Application
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
      Sample
      Mouse Tissue sections (Spleen)
      Antigen retrieval step
      Enzymatic - Buffer/Enzyme Used: ProK
      Permeabilization
      No
      Specification
      Spleen
      Blocking step
      PB ab64226 as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: RT°C
      Fixative
      Formaldehyde
      Read More

      Abcam user community

      Verified customer

      Submitted Feb 19 2018

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-F4/80 antibody [SP115]

      Poor
      Abreviews
      Abreviews
      abreview image
      Application
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
      Sample
      Rat Tissue sections (kidney)
      Antigen retrieval step
      None
      Permeabilization
      Yes - 0.1% Triton-X 100
      Specification
      kidney
      Blocking step
      Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
      Fixative
      Paraformaldehyde
      Read More

      Abcam user community

      Verified customer

      Submitted Nov 28 2017

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-F4/80 antibody [SP115]

      Excellent
      Abreviews
      Abreviews
      abreview image
      Application
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
      Sample
      Mouse Tissue sections (uterus and spleen (multi-block))
      Antigen retrieval step
      Heat mediated - Buffer/Enzyme Used: Dako high pH buffer
      Permeabilization
      No
      Specification
      uterus and spleen (multi-block)
      Blocking step
      Vector casein solution as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 24°C
      Fixative
      Paraformaldehyde
      Read More

      Dr. Henry King

      Verified customer

      Submitted Aug 07 2017

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-F4/80 antibody [SP115]

      Good
      Abreviews
      Abreviews
      abreview image
      Application
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
      Sample
      Mouse Tissue sections (spleen)
      Antigen retrieval step
      Heat mediated - Buffer/Enzyme Used: Tris/EDTA pH 9.0
      Permeabilization
      No
      Specification
      spleen
      Blocking step
      Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 20% · Temperature: RT°C
      Fixative
      10% NBF
      Read More

      Abcam user community

      Verified customer

      Submitted Jul 11 2017

      1-10 of 28 Abreviews or Q&A

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