• Product name
  • Description
    Rabbit polyclonal to FAAH1
  • Host species
  • Tested applications
    Suitable for: ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Pig
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 500 to the C-terminus of Human FAAH1.

  • Positive control
    • This antibody gave a positive signal in the following lysates: Brain (Human) Tissue Lysate; Small Intestine (Human) Tissue Lysate; Pancreas (Human) Tissue Lysate; Skeletal Muscle (Human) Tissue Lysate; Testis (Human) Tissue Lysate; A431 Whole Cell Lysate



Our Abpromise guarantee covers the use of ab98065 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 1 mg/ml. Detects a band of approximately 63 kDa (predicted molecular weight: 63 kDa).


  • Function
    Degrades bioactive fatty acid amides like oleamide, the endogenous cannabinoid, anandamide and myristic amide to their corresponding acids, thereby serving to terminate the signaling functions of these molecules. Hydrolyzes polyunsaturated substrate anandamide preferentially as compared to monounsaturated substrates.
  • Tissue specificity
    Highly expressed in the brain, small intestine, pancreas, skeletal muscle and testis. Also expressed in the kidney, liver, lung, placenta and prostate.
  • Sequence similarities
    Belongs to the amidase family.
  • Cellular localization
    Endomembrane system. Cytoplasm > cytoskeleton. Seems to be attached to intracellular membranes and a portion of the cytoskeletal network.
  • Information by UniProt
  • Database links
  • Alternative names
    • Anandamide amidohydrolase 1 antibody
    • Anandamide amidohydrolase antibody
    • FA2H antibody
    • FAAH antibody
    • FAAH-1 antibody
    • FAAH1_HUMAN antibody
    • Fatty acid 2-hydroxylase antibody
    • Fatty acid alpha-hydroxylase antibody
    • Fatty acid amide hydrolase antibody
    • Fatty-acid amide hydrolase 1 antibody
    • MGC102823 antibody
    • MGC138146 antibody
    • Oleamide hydrolase 1 antibody
    • Oleamide hydrolase antibody
    see all


  • All lanes : Anti-FAAH1 antibody (ab98065) at 1 µg/ml

    Lane 1 : Human small intestine tissue lysate - total protein (ab29276)
    Lane 2 : Liver (Mouse) Nuclear Lysate - normal tissue (ab29300)
    Lane 3 : Human testis tissue lysate - total protein (ab30257)
    Lane 4 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    All lanes : Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 63 kDa
    Observed band size: 63 kDa
    Additional bands at: 20 kDa, 25 kDa, 40 kDa, 50 kDa, 70 kDa. We are unsure as to the identity of these extra bands.

    Exposure time: 90 seconds
  • ICC/IF image of ab98065 stained SKNSH cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab98065, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 100% methanol fixed (5 min) SKNSH cells at 5µg/ml.


ab98065 has not yet been referenced specifically in any publications.

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