Key features and details
- Rabbit polyclonal to FAAP24
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-FAAP24 antibody
DescriptionRabbit polyclonal to FAAP24
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse
- This antibody gave a positive signal in HeLa Nuclear extract, Jurkat Nuclear extract and Irradiated HeLa whole cell lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab48124 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 25 kDa (predicted molecular weight: 24 kDa).|
RelevanceFAAP24 was identified as a protein that interacts with FANCM as part of the core Fanconi Anemia complex. It is homologous to the XPF family of endonucleases. FAAP24 is required for normal levels of FANCD2 ubiquitination after DNA damage. Knockdown of FAAP24 leads to chromosomal instability.
- C19ORF40 antibody
- C19orf40 chromosome 19 open reading frame 40 antibody
- FAAP24 antibody
All lanes : Anti-FAAP24 antibody (ab48124) at 1 µg/ml
Lane 1 : Jurkat nuclear extract lysate (ab14844)
Lane 2 : HeLa (Human epithelial carcinoma cell line) Nuclear Lysate
Lane 3 : HeLa Whole Cell Lysate - Irradiated (5Gy
Lysates/proteins at 20 µg per lane.
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Predicted band size: 24 kDa
Observed band size: 25 kDa why is the actual band size different from the predicted?
Additional bands at: 120 kDa, 40 kDa. We are unsure as to the identity of these extra bands.
Abcam recommends using milk as the blocking agent. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.
ab48124 has not yet been referenced specifically in any publications.