Anti-FABP-1 antibody [8D2BB12] (ab113693)
Key features and details
- Mouse monoclonal [8D2BB12] to FABP-1
- Suitable for: ICC
- Reacts with: Human
- Isotype: IgG2b
Overview
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Product name
Anti-FABP-1 antibody [8D2BB12]
See all FABP-1 primary antibodies -
Description
Mouse monoclonal [8D2BB12] to FABP-1 -
Host species
Mouse -
Tested applications
Suitable for: ICCmore details -
Species reactivity
Reacts with: Human -
Immunogen
Tissue, cells or virus. This information is considered to be commercially sensitive.
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Positive control
- Fibroblast cells
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General notes
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Product was previously marketed under the MitoSciences sub-brand.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.5
Preservative: 0.02% Sodium azide
Constituent: HEPES buffered saline -
Concentration information loading...
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Purity
Proprietary Purification -
Purification notes
The antibody was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation. Purity >95% by SDS-PAGE. -
Clonality
Monoclonal -
Clone number
8D2BB12 -
Isotype
IgG2b -
Light chain type
kappa -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab113693 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC |
Use a concentration of 2 µg/ml.
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Notes |
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ICC
Use a concentration of 2 µg/ml. |
Target
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Function
Plays a key role in amino acid metabolism. Important for metabolite exchange between mitochondria and cytosol. Facilitates cellular uptake of long-chain free fatty acids. -
Sequence similarities
Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family. -
Cellular localization
Mitochondrion matrix. Cell membrane. Exposure to alcohol promotes translocation to the cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 2806 Human
- Omim: 138150 Human
- SwissProt: P00505 Human
- Unigene: 599470 Human
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Alternative names
- AATM_HUMAN antibody
- AL022787 antibody
- Aspartate aminotransferase 2 antibody
see all
Images
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ab113693 [8D2BB12] stained human fibroblast. The cells were paraformaldehyde fixed (4%, 20 min) and Triton X-100 permeabilized (0.1%, 15min). The cells were incubated with the antibody (8D2BB12, 2µg/ml) for 2h at room temperature or over night at 4°C. The secondary antibody was (red) Alexa Fluor® 594 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. 10% Goat serum was used as the blocking agent for all blocking steps. The target protein locates to the mitochondrial matrix.
Protocols
Datasheets and documents
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Datasheet download
References (0)
ab113693 has not yet been referenced specifically in any publications.