Anti-FABP-1 antibody (ab153924)
- Datasheet
- References (1)
- Protocols
Overview
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Product name
Anti-FABP-1 antibody
See all FABP-1 primary antibodies -
Description
Rabbit polyclonal to FABP-1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment corresponding to Human FABP-1 aa 174-392.
Database link: P00505 -
Positive control
- Recombinant Human FABP-1 protein (ab140064) can be used as a positive control in WB. HepG2, Mouse Neuro2A and Rat lung whole cell lysates; HeLa cells; Human hepatoma, Rat RT2 xenograft and Human SkHep1 xenograft tissues.
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General notes
Previously labelled as GOT2.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78% PBS, 20% Glycerol, 1% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab153924 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/3000. Predicted molecular weight: 48 kDa. | |
| IHC-P | 1/100 - 1/1000. | |
| ICC/IF | 1/100 - 1/1000. |
Target
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Function
Plays a key role in amino acid metabolism. Important for metabolite exchange between mitochondria and cytosol. Facilitates cellular uptake of long-chain free fatty acids. -
Sequence similarities
Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family. -
Cellular localization
Mitochondrion matrix. Cell membrane. Exposure to alcohol promotes translocation to the cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 2806 Human
- Entrez Gene: 14719 Mouse
- Entrez Gene: 25721 Rat
- Omim: 138150 Human
- SwissProt: P00505 Human
- SwissProt: P05202 Mouse
- SwissProt: P00507 Rat
- Unigene: 599470 Human
see all -
Alternative names
- AATM_HUMAN antibody
- AL022787 antibody
- Aspartate aminotransferase 2 antibody
see all
Images
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Western blot - Anti-FABP-1 antibody (ab153924)Anti-FABP-1 antibody (ab153924) at 1/1000 dilution + HepG2 whole cell lysate at 30 µg
Predicted band size: 48 kDa
10% SDS PAGE -
Western blot - Anti-FABP-1 antibody (ab153924)Anti-FABP-1 antibody (ab153924) at 1/1000 dilution + Mouse Neuro2A whole cell lysate at 30 µg
Predicted band size: 48 kDa
10 % SDS-PAGE -
Western blot - Anti-FABP-1 antibody (ab153924)Anti-FABP-1 antibody (ab153924) at 1/1000 dilution + Rat lung lysate at 50 µg
Predicted band size: 48 kDa
10 % SDS-PAGE -
Immunocytochemistry/ Immunofluorescence - Anti-FABP-1 antibody (ab153924)Immunofluorescence analysis of methanol-fixed HeLa cells, labeling FABP-1 with ab153924 at 1/500 dilution. Lower image shows cells co-stained with Hoechst 33342.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FABP-1 antibody (ab153924)Immunohistochemical analysis of paraffin-embedded Rat RT2 xenograft tissue, labeling FABP-1 with ab153924 at 1/500 dilution.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FABP-1 antibody (ab153924)Immunohistochemical analysis of paraffin-embedded Human SKHep1 xenograft tissue, labeling FABP-1 with ab153924 at 1/500 dilution.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FABP-1 antibody (ab153924)Immunohistochemical analysis of paraffin-embedded Human hepatoma tissue, labeling FABP-1 with ab153924 at 1/500 dilution.
Protocols
References
This product has been referenced in:
- Du F et al. SOX12 promotes colorectal cancer cell proliferation and metastasis by regulating asparagine synthesis. Cell Death Dis 10:239 (2019). Read more (PubMed: 30858360) »
