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    fabp-1-antibody-ab153924.pdf

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Signal Transduction Metabolism Amino Acids
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Anti-FABP-1 antibody (ab153924)

  • Datasheet
  • SDS
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Western blot - Anti-FABP-1 antibody (ab153924)
  • Western blot - Anti-FABP-1 antibody (ab153924)
  • Western blot - Anti-FABP-1 antibody (ab153924)
  • Immunocytochemistry/ Immunofluorescence - Anti-FABP-1 antibody (ab153924)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FABP-1 antibody (ab153924)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FABP-1 antibody (ab153924)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FABP-1 antibody (ab153924)

Key features and details

  • Rabbit polyclonal to FABP-1
  • Suitable for: WB, IHC-P, ICC/IF
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-FABP-1 antibody
    See all FABP-1 primary antibodies
  • Description

    Rabbit polyclonal to FABP-1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment corresponding to Human FABP-1 aa 174-392.
    Database link: P00505

  • Positive control

    • Recombinant Human FABP-1 protein (ab140064) can be used as a positive control in WB. HepG2, Mouse Neuro2A and Rat lung whole cell lysates; HeLa cells; Human hepatoma, Rat RT2 xenograft and Human SkHep1 xenograft tissues.
  • General notes

    Previously labelled as GOT2. 

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 78.99% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Metabolism
    • Amino Acids
    • Cancer
    • Cancer Metabolism
    • Metabolic signaling pathway
    • Metabolism of carbohydrates
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Carbohydrate metabolism
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Amino acid metabolism

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant Human FABP-1 protein (ab170592)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab153924 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/500 - 1/3000. Predicted molecular weight: 48 kDa.
IHC-P
1/100 - 1/1000.
ICC/IF
1/100 - 1/1000.
Notes
WB
1/500 - 1/3000. Predicted molecular weight: 48 kDa.
IHC-P
1/100 - 1/1000.
ICC/IF
1/100 - 1/1000.

Target

  • Function

    Plays a key role in amino acid metabolism. Important for metabolite exchange between mitochondria and cytosol. Facilitates cellular uptake of long-chain free fatty acids.
  • Sequence similarities

    Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family.
  • Cellular localization

    Mitochondrion matrix. Cell membrane. Exposure to alcohol promotes translocation to the cell membrane.
  • Target information above from: UniProt accession P00505 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 2806 Human
    • Entrez Gene: 14719 Mouse
    • Entrez Gene: 25721 Rat
    • Omim: 138150 Human
    • SwissProt: P00505 Human
    • SwissProt: P05202 Mouse
    • SwissProt: P00507 Rat
    • Unigene: 599470 Human
    • Unigene: 230169 Mouse
    • Unigene: 98650 Rat
    see all
  • Alternative names

    • AATM_HUMAN antibody
    • AL022787 antibody
    • Aspartate aminotransferase 2 antibody
    • Aspartate aminotransferase antibody
    • Aspartate aminotransferase, mitochondrial antibody
    • Aspartate transaminase 2 antibody
    • ASPATA antibody
    • EC 2.6.1.1 antibody
    • FABP 1 antibody
    • FABP pm antibody
    • FABP-1 antibody
    • FABPpm antibody
    • Fatty acid binding protein antibody
    • Fatty acid-binding protein antibody
    • FLJ40994 antibody
    • Glutamate oxaloacetate transaminase 2 antibody
    • Glutamate oxaloacetate transaminase 2, mitochondrial antibody
    • Glutamate oxaloacetate transaminase, mitochondrial antibody
    • Glutamic oxaloacetic transaminase 2, mitochondrial (aspartate aminotransferase 2) antibody
    • Got 2 antibody
    • GOT2 antibody
    • KAT4 antibody
    • KATIV antibody
    • kynurenine aminotransferase 4 antibody
    • Kynurenine aminotransferase IV antibody
    • kynurenine--oxoglutarate transaminase 4 antibody
    • kynurenine--oxoglutarate transaminase IV antibody
    • mAspAT antibody
    • MGC102129 antibody
    • MGC115763 antibody
    • mitAAT antibody
    • mitochondrial antibody
    • Mitochondrial aspartate aminotransferase antibody
    • OTTMUSP00000017748 antibody
    • Plasma membrane fatty acid binding protein antibody
    • Plasma membrane-associated fatty acid-binding protein antibody
    • Transaminase A antibody
    see all

Images

  • Western blot - Anti-FABP-1 antibody (ab153924)
    Western blot - Anti-FABP-1 antibody (ab153924)
    Anti-FABP-1 antibody (ab153924) at 1/1000 dilution + HepG2 whole cell lysate at 30 µg

    Predicted band size: 48 kDa



    10% SDS PAGE
  • Western blot - Anti-FABP-1 antibody (ab153924)
    Western blot - Anti-FABP-1 antibody (ab153924)
    Anti-FABP-1 antibody (ab153924) at 1/1000 dilution + Mouse Neuro2A whole cell lysate at 30 µg

    Predicted band size: 48 kDa



    10 % SDS-PAGE
  • Western blot - Anti-FABP-1 antibody (ab153924)
    Western blot - Anti-FABP-1 antibody (ab153924)
    Anti-FABP-1 antibody (ab153924) at 1/1000 dilution + Rat lung lysate at 50 µg

    Predicted band size: 48 kDa



    10 % SDS-PAGE
  • Immunocytochemistry/ Immunofluorescence - Anti-FABP-1 antibody (ab153924)
    Immunocytochemistry/ Immunofluorescence - Anti-FABP-1 antibody (ab153924)

    Immunofluorescence analysis of methanol-fixed HeLa cells, labeling FABP-1 with ab153924 at 1/500 dilution. Lower image shows cells co-stained with Hoechst 33342.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FABP-1 antibody (ab153924)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FABP-1 antibody (ab153924)

    Immunohistochemical analysis of paraffin-embedded Rat RT2 xenograft tissue, labeling FABP-1 with ab153924 at 1/500 dilution.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FABP-1 antibody (ab153924)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FABP-1 antibody (ab153924)

    Immunohistochemical analysis of paraffin-embedded Human SKHep1 xenograft tissue, labeling FABP-1 with ab153924 at 1/500 dilution.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FABP-1 antibody (ab153924)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FABP-1 antibody (ab153924)

    Immunohistochemical analysis of paraffin-embedded Human hepatoma tissue, labeling FABP-1 with ab153924 at 1/500 dilution.

Protocols

  • Immunohistochemistry protocols
  • Immunocytochemistry & immunofluorescence protocols
  • Western blot protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (1)

Publishing research using ab153924? Please let us know so that we can cite the reference in this datasheet.

ab153924 has been referenced in 1 publication.

  • Du F  et al. SOX12 promotes colorectal cancer cell proliferation and metastasis by regulating asparagine synthesis. Cell Death Dis 10:239 (2019). PubMed: 30858360

Customer reviews and Q&As

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