Anti-FABP-1 antibody (ab153924)
Key features and details
- Rabbit polyclonal to FABP-1
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-FABP-1 antibody
See all FABP-1 primary antibodies -
Description
Rabbit polyclonal to FABP-1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment corresponding to Human FABP-1 aa 174-392.
Database link: P00505 -
Positive control
- Recombinant Human FABP-1 protein (ab140064) can be used as a positive control in WB. HepG2, Mouse Neuro2A and Rat lung whole cell lysates; HeLa cells; Human hepatoma, Rat RT2 xenograft and Human SkHep1 xenograft tissues.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78.99% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab153924 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/3000. Predicted molecular weight: 48 kDa.
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IHC-P |
1/100 - 1/1000.
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ICC/IF |
1/100 - 1/1000.
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Notes |
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WB
1/500 - 1/3000. Predicted molecular weight: 48 kDa. |
IHC-P
1/100 - 1/1000. |
ICC/IF
1/100 - 1/1000. |
Target
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Function
Plays a key role in amino acid metabolism. Important for metabolite exchange between mitochondria and cytosol. Facilitates cellular uptake of long-chain free fatty acids. -
Sequence similarities
Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family. -
Cellular localization
Mitochondrion matrix. Cell membrane. Exposure to alcohol promotes translocation to the cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 2806 Human
- Entrez Gene: 14719 Mouse
- Entrez Gene: 25721 Rat
- Omim: 138150 Human
- SwissProt: P00505 Human
- SwissProt: P05202 Mouse
- SwissProt: P00507 Rat
- Unigene: 599470 Human
see all -
Alternative names
- AATM_HUMAN antibody
- AL022787 antibody
- Aspartate aminotransferase 2 antibody
see all
Images
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Anti-FABP-1 antibody (ab153924) at 1/1000 dilution + HepG2 whole cell lysate at 30 µg
Predicted band size: 48 kDa
10% SDS PAGE -
Anti-FABP-1 antibody (ab153924) at 1/1000 dilution + Mouse Neuro2A whole cell lysate at 30 µg
Predicted band size: 48 kDa
10 % SDS-PAGE -
Anti-FABP-1 antibody (ab153924) at 1/1000 dilution + Rat lung lysate at 50 µg
Predicted band size: 48 kDa
10 % SDS-PAGE -
Immunofluorescence analysis of methanol-fixed HeLa cells, labeling FABP-1 with ab153924 at 1/500 dilution. Lower image shows cells co-stained with Hoechst 33342.
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Immunohistochemical analysis of paraffin-embedded Rat RT2 xenograft tissue, labeling FABP-1 with ab153924 at 1/500 dilution.
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Immunohistochemical analysis of paraffin-embedded Human SKHep1 xenograft tissue, labeling FABP-1 with ab153924 at 1/500 dilution.
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Immunohistochemical analysis of paraffin-embedded Human hepatoma tissue, labeling FABP-1 with ab153924 at 1/500 dilution.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (4)
ab153924 has been referenced in 4 publications.
- Błachnio-Zabielska AU et al. CerS1 but Not CerS5 Gene Silencing, Improves Insulin Sensitivity and Glucose Uptake in Skeletal Muscle. Cells 11:N/A (2022). PubMed: 35053322
- Supruniuk E et al. Pyrroloquinoline Quinone Modifies Lipid Profile, but Not Insulin Sensitivity, of Palmitic Acid-Treated L6 Myotubes. Int J Mol Sci 21:N/A (2020). PubMed: 33171690
- Du F et al. SOX12 promotes colorectal cancer cell proliferation and metastasis by regulating asparagine synthesis. Cell Death Dis 10:239 (2019). PubMed: 30858360
- Eskelund A et al. Drugs with antidepressant properties affect tryptophan metabolites differently in rodent models with depression-like behavior. J Neurochem 142:118-131 (2017). PubMed: 28407315