Product nameAnti-FABP-1 antibody
See all FABP-1 primary antibodies
DescriptionRabbit polyclonal to FABP-1
Tested applicationsSuitable for: IHC-P, WBmore details
Unsuitable for: IP
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Rabbit, Horse, Chicken, Cow, Pig, Orangutan, Xenopus tropicalis
Synthetic peptide within Human FABP-1 aa 380-430. The exact sequence is proprietary. NP_002071.2
Database link: P00505
- WB: HeLa, HEK-293T, Jurkat, TCMK-1 and NIH/3T3 whole cell lysate. IHC-P: Human prostate carcinoma and mouse renal cell carcinoma tissue.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab245593 was affinity purified using an epitope specific to FABP-1 immobilized on solid support.
Our Abpromise guarantee covers the use of ab245593 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||1/2000 - 1/10000. Predicted molecular weight: 48 kDa.|
FunctionPlays a key role in amino acid metabolism. Important for metabolite exchange between mitochondria and cytosol. Facilitates cellular uptake of long-chain free fatty acids.
Sequence similaritiesBelongs to the class-I pyridoxal-phosphate-dependent aminotransferase family.
Cellular localizationMitochondrion matrix. Cell membrane. Exposure to alcohol promotes translocation to the cell membrane.
- Information by UniProt
- AATM_HUMAN antibody
- AL022787 antibody
- Aspartate aminotransferase 2 antibody
All lanes : Anti-FABP-1 antibody (ab245593) at 0.1 µg/ml
Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 4 : TCMK-1 (Mouse kidney epithelial cell line) whole cell lysate
Lane 5 : NIH/3T3 (Mouse embryo fibroblast cell line) whole cell lysate
Lysates/proteins at 50 µg per lane.
Predicted band size: 48 kDa
Exposure time: 30 seconds
Formalin-fixed, paraffin-embedded human prostate carcinoma tissue stained for FABP-1 with ab245593 at a 1/1000 dilution in immunohistochemical analysis. DAB staining. Counterstained using hematoxylin (blue).
Formalin-fixed, paraffin-embedded mouse renal cell carcinoma tissue stained for FABP-1 with ab245593 at a 1/1000 dilution in immunohistochemical analysis. DAB staining.
ab245593 has not yet been referenced specifically in any publications.