Key features and details
- Rabbit polyclonal to FABP-1
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-FABP-1 antibody
See all FABP-1 primary antibodies
DescriptionRabbit polyclonal to FABP-1
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Rabbit, Horse, Chicken, Cow, Pig, Orangutan, Xenopus tropicalis
Synthetic peptide within Human FABP-1 aa 380-430. The exact sequence is proprietary. NP_002071.2
Database link: P00505
- WB: HeLa, HEK-293T, Jurkat, TCMK-1 and NIH/3T3 whole cell lysate. IHC-P: Human prostate carcinoma and mouse renal cell carcinoma tissue.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab245593 was affinity purified using an epitope specific to FABP-1 immobilized on solid support.
Our Abpromise guarantee covers the use of ab245593 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||1/2000 - 1/10000. Predicted molecular weight: 48 kDa.|
FunctionPlays a key role in amino acid metabolism. Important for metabolite exchange between mitochondria and cytosol. Facilitates cellular uptake of long-chain free fatty acids.
Sequence similaritiesBelongs to the class-I pyridoxal-phosphate-dependent aminotransferase family.
Cellular localizationMitochondrion matrix. Cell membrane. Exposure to alcohol promotes translocation to the cell membrane.
- Information by UniProt
- AATM_HUMAN antibody
- AL022787 antibody
- Aspartate aminotransferase 2 antibody
All lanes : Anti-FABP-1 antibody (ab245593) at 0.1 µg/ml
Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 4 : TCMK-1 (Mouse kidney epithelial cell line) whole cell lysate
Lane 5 : NIH/3T3 (Mouse embryo fibroblast cell line) whole cell lysate
Lysates/proteins at 50 µg per lane.
Predicted band size: 48 kDa
Exposure time: 30 seconds
Formalin-fixed, paraffin-embedded human prostate carcinoma tissue stained for FABP-1 with ab245593 at a 1/1000 dilution in immunohistochemical analysis. DAB staining. Counterstained using hematoxylin (blue).
Formalin-fixed, paraffin-embedded mouse renal cell carcinoma tissue stained for FABP-1 with ab245593 at a 1/1000 dilution in immunohistochemical analysis. DAB staining.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab245593 has not yet been referenced specifically in any publications.