Product nameAnti-FABP-1 antibody [EPR12354(B)]
See all FABP-1 primary antibodies
DescriptionRabbit monoclonal [EPR12354(B)] to FABP-1
Tested applicationsSuitable for: WB, IHC-P, IP, Flow Cytmore details
Unsuitable for: ICC/IF
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human FABP-1 aa 1-100 (Cysteine residue). The exact sequence is proprietary.
Database link: P00505
- Human fetal liver lysate; 293T, HepG2 and HeLa cell lysate; Human liver and urinary bladder carcinoma tissue; permeabilized 293T cells.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab171739 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Predicted molecular weight: 48 kDa.|
|IHC-P||1/50 - 1/100.|
|IP||1/10 - 1/100.|
|Flow Cyt||1/100 - 1/500.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
FunctionPlays a key role in amino acid metabolism. Important for metabolite exchange between mitochondria and cytosol. Facilitates cellular uptake of long-chain free fatty acids.
Sequence similaritiesBelongs to the class-I pyridoxal-phosphate-dependent aminotransferase family.
Cellular localizationMitochondrion matrix. Cell membrane. Exposure to alcohol promotes translocation to the cell membrane.
- Information by UniProt
- AATM_HUMAN antibody
- AL022787 antibody
- Aspartate aminotransferase 2 antibody
Flow cytometric analysis of permeabilized 293T cells labeling FABP-1 using ab171739 at 1/100 (red) or a rabbit IgG (negative) (green).
Immunohistochemistry of paraffin-embedded Human urinary bladder carcinoma tissue labeling FABP-1 using ab171739 at 1/50 dilution.
Immunohistochemistry of paraffin-embedded Human liver tissue labeling FABP-1 using ab171739 at 1/50 dilution.
Western blot analysis on immunoprecipitation pellet from Human fetal liver lysate (lane 1) labeling FABP-1 using ab171739 at 1/10 dilution, and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG. Lane 2: 1X PBS (negative control).
All lanes : Anti-FABP-1 antibody [EPR12354(B)] (ab171739) at 1/1000 dilution
Lane 1 : Human fetal liver lysate
Lane 2 : 293T cell lysate
Lane 3 : HepG2 cell lysate
Lane 4 : HeLa cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 48 kDa
This product has been referenced in:
- Schatton D et al. CLUH regulates mitochondrial metabolism by controlling translation and decay of target mRNAs. J Cell Biol 216:675-693 (2017). WB ; Mouse . Read more (PubMed: 28188211) »
- McKenzie AT et al. Multiscale network modeling of oligodendrocytes reveals molecular components of myelin dysregulation in Alzheimer's disease. Mol Neurodegener 12:82 (2017). WB, IHC-P ; Mouse . Read more (PubMed: 29110684) »