Overview

  • Product name

    Anti-FABP-1 antibody [EPR12354(B)]
    See all FABP-1 primary antibodies
  • Description

    Rabbit monoclonal [EPR12354(B)] to FABP-1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, IPmore details
    Unsuitable for: Flow Cyt
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human FABP-1 aa 1-100 (Cysteine residue). The exact sequence is proprietary.
    Database link: P00505

  • Positive control

    • WB: HeLa, Human liver, HEPG2, HEK-293T, Mouse brain, Rat brain, Mouse liver, and Rat liver lysates; IHC-P: human liver and urinary bladder carcinoma, mouse kidney, and rat stomach tissues; IP: Human fetal liver lysate.
  • General notes

     Previously labelled as GOT2. 

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab171739 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 48 kDa.
IHC-P 1/4000.

See IHC antigen retrieval protocols.

For unpurified use at 1/50 - 1/100.

IP 1/10 - 1/100.
  • Application notes
    Is unsuitable for Flow Cyt.
  • Target

    • Function

      Plays a key role in amino acid metabolism. Important for metabolite exchange between mitochondria and cytosol. Facilitates cellular uptake of long-chain free fatty acids.
    • Sequence similarities

      Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family.
    • Cellular localization

      Mitochondrion matrix. Cell membrane. Exposure to alcohol promotes translocation to the cell membrane.
    • Information by UniProt
    • Database links

    • Alternative names

      • AATM_HUMAN antibody
      • AL022787 antibody
      • Aspartate aminotransferase 2 antibody
      • Aspartate aminotransferase antibody
      • Aspartate aminotransferase, mitochondrial antibody
      • Aspartate transaminase 2 antibody
      • ASPATA antibody
      • EC 2.6.1.1 antibody
      • FABP 1 antibody
      • FABP pm antibody
      • FABP-1 antibody
      • FABPpm antibody
      • Fatty acid binding protein antibody
      • Fatty acid-binding protein antibody
      • FLJ40994 antibody
      • Glutamate oxaloacetate transaminase 2 antibody
      • Glutamate oxaloacetate transaminase 2, mitochondrial antibody
      • Glutamate oxaloacetate transaminase, mitochondrial antibody
      • Glutamic oxaloacetic transaminase 2, mitochondrial (aspartate aminotransferase 2) antibody
      • Got 2 antibody
      • GOT2 antibody
      • KAT4 antibody
      • KATIV antibody
      • kynurenine aminotransferase 4 antibody
      • Kynurenine aminotransferase IV antibody
      • kynurenine--oxoglutarate transaminase 4 antibody
      • kynurenine--oxoglutarate transaminase IV antibody
      • mAspAT antibody
      • MGC102129 antibody
      • MGC115763 antibody
      • mitAAT antibody
      • mitochondrial antibody
      • Mitochondrial aspartate aminotransferase antibody
      • OTTMUSP00000017748 antibody
      • Plasma membrane fatty acid binding protein antibody
      • Plasma membrane-associated fatty acid-binding protein antibody
      • Transaminase A antibody
      see all

    Images

    • All lanes : Anti-FABP-1 antibody [EPR12354(B)] (ab171739) at 1/1000 dilution (Purified)

      Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates
      Lane 2 : Mouse brain lysates
      Lane 3 : Rat brain lysates
      Lane 4 : Mouse liver lysates
      Lane 5 : Rat liver lysates

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

      Predicted band size: 48 kDa
      Observed band size: 43 kDa
      why is the actual band size different from the predicted?

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat stomach tissue sections labeling FABP-1 with purified ab171739 at 1/4000 dilution (0.25 µg/ml). Heat mediated antigen retrieval was performed Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
    • Anti-FABP-1 antibody [EPR12354(B)] (ab171739) at 1/1000 dilution + Recombinant human FABP-1 protein (ab206788) at 1/0.015 dilution with 5% NFDM/TBST

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051)

      Predicted band size: 48 kDa
      Observed band size: 47 kDa why is the actual band size different from the predicted?


      Exposure time: 10 seconds
    • ab171739 (purified ) at 1/50 dilution (2 µg) immunoprecipitating FABP-1 in Human fetal liver lysate.
      Lane 1 (input): Human fetal liver lysate 10 µg
      Lane 2 (+): ab171739 & Human fetal liver lysate
      Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab171739 in Human fetal liver lysate
      For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/1000 dilution.
      Blocking and diluting buffer: 5% NFDM/TBST.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse kidney tissue sections labeling FABP-1 with purified ab171739 at 1/4000 dilution (0.25 µg/ml). Heat mediated antigen retrieval was performed Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human liver tissue sections labeling FABP-1 with purified ab171739 at 1/4000 dilution (0.25 µg/ml). Heat mediated antigen retrieval was performed Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
    • All lanes : Anti-FABP-1 antibody [EPR12354(B)] (ab171739) at 1/1000 dilution (unpurified)

      Lane 1 : Human fetal liver lysate
      Lane 2 : 293T cell lysate
      Lane 3 : HepG2 cell lysate
      Lane 4 : HeLa cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 48 kDa

    • Immunohistochemistry of paraffin-embedded Human urinary bladder carcinoma tissue labeling FABP-1 using ab171739 (unpurified) at 1/50 dilution.

    • Immunohistochemistry of paraffin-embedded Human liver tissue labeling FABP-1 using ab171739 (unpurified) at 1/50 dilution.

    • Western blot analysis on immunoprecipitation pellet from Human fetal liver lysate (lane 1) labeling FABP-1 using ab171739 (unpurified) at 1/10 dilution, and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG. Lane 2: 1X PBS (negative control).

    References

    This product has been referenced in:

    • Holt MC  et al. Biochemical Characterization and Structure-Based Mutational Analysis Provide Insight into the Binding and Mechanism of Action of Novel Aspartate Aminotransferase Inhibitors. Biochemistry 57:6604-6614 (2018). Read more (PubMed: 30365304) »
    • Schatton D  et al. CLUH regulates mitochondrial metabolism by controlling translation and decay of target mRNAs. J Cell Biol 216:675-693 (2017). WB ; Mouse . Read more (PubMed: 28188211) »
    See all 4 Publications for this product

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