Anti-FABP12 antibody [EPR10536] (Rabbit Monoclonal) (ab155089)

Product name

Anti-FABP12 antibody [EPR10536]
See all FABP12 primary antibodies
Description

Rabbit monoclonal [EPR10536] to FABP12
Host species

Rabbit
Tested applications

Suitable for: WB, Flow Cyt, IP, ICC/IFmore details
Unsuitable for: IHC-P
Species reactivity

Reacts with: Mouse, Rat, Human
Immunogen

Synthetic peptide, corresponding to residues in Human FABP12 (UniProt: A6NFH5).
Positive control
- Rat eyeball, Mouse eyeball, Human fetal brain and Jurkat lysates; Jurkat cells.
General notes

Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab^® patents.
This product is a recombinant rabbit monoclonal antibody.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at -20ºC.
Storage buffer

Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EPR10536
Isotype

IgG
Research areas

Our Abpromise guarantee covers the use of ab155089 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application	Abreviews	Notes
WB		1/1000 - 1/10000. Predicted molecular weight: 16 kDa.
Flow Cyt		1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
IP		1/10 - 1/100.
ICC/IF		1/50 - 1/100.

Application notes

Is unsuitable for IHC-P.

Function

May play a role in lipid transport.
Tissue specificity

Expressed in a number of retinoblastoma cell lines.
Sequence similarities

Belongs to the calycin superfamily. Fatty-acid binding protein (FABP) family.
Developmental stage

Not detected in fetal tissues.
Target information above from: UniProt accession A6NFH5 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
Database links
- Entrez Gene: 646486 Human
- Entrez Gene: 75497 Mouse
- Entrez Gene: 499570 Rat
- SwissProt: A6NFH5 Human
- SwissProt: Q9DAK4 Mouse
- SwissProt: B7SUM8 Rat
- Unigene: 586370 Human
- Unigene: 47052 Mouse
- Unigene: 229045 Rat
see all
Alternative names
- FABP12 antibody
- Fatty acid binding protein ORF antibody
- Fatty acid-binding protein 12 antibody
- FBP12_HUMAN antibody
- Intracellular fatty acid binding protein FABP12 antibody
- Probable fatty acid binding protein ENSP00000353650 antibody
see all

Western blot - Anti-FABP12 antibody [EPR10536] (ab155089)

All lanes : Anti-FABP12 antibody [EPR10536] (ab155089) at 1/1000 dilution

Lane 1 : Rat eyeball lysate
Lane 2 : Mouse eyeball lysate
Lane 3 : Human fetal brain lysate
Lane 4 : Jurkat cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 16 kDa
Immunocytochemistry/ Immunofluorescence - Anti-FABP12 antibody [EPR10536] (ab155089)

Immunofluorescent analysis of Jurkat cells labeling FABP12 with ab155089 at 1/50 dilution.
Flow Cytometry - Anti-FABP12 antibody [EPR10536] (ab155089)

Flow cytometric analysis of permeabilized Jurkat cells, labeling FABP12 with ab155089 at 1/10 dilution (red) compared with a Rabbit IgG control (green).

ab155089 has not yet been referenced specifically in any publications.

Publishing research using ab155089? Please let us know so that we can cite the reference in this datasheet.

Anti-FABP12 antibody [EPR10536] (ab155089)