Anti-FABP4 antibody [9B8D] (ab93945)
Key features and details
- Mouse monoclonal [9B8D] to FABP4
- Suitable for: WB, Flow Cyt
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-FABP4 antibody [9B8D]
See all FABP4 primary antibodies -
Description
Mouse monoclonal [9B8D] to FABP4 -
Host species
Mouse -
Tested applications
Suitable for: WB, Flow Cytmore details -
Species reactivity
Reacts with: Human -
Immunogen
Purified recombinant fragment of Human FABP4 expressed in E. coli.
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Positive control
- FABP4-hIgGFc transfected HEK293 cell lysate
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Protein G purified -
Purification notes
Purified from tissue culture supernatant. -
Clonality
Monoclonal -
Clone number
9B8D -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab93945 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/2000. Predicted molecular weight: 15 kDa.
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Flow Cyt |
1/100.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
Notes |
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WB
1/500 - 1/2000. Predicted molecular weight: 15 kDa. |
Flow Cyt
1/100. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
Target
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Function
Lipid transport protein in adipocytes. Binds both long chain fatty acids and retinoic acid. Delivers long-chain fatty acids and retinoic acid to their cognate receptors in the nucleus. -
Sequence similarities
Belongs to the calycin superfamily. Fatty-acid binding protein (FABP) family. -
Domain
Forms a beta-barrel structure that accommodates hydrophobic ligands in its interior. -
Cellular localization
Cytoplasm. Nucleus. Depending on the nature of the ligand, a conformation change exposes a nuclear localization motif and the protein is transported into the nucleus. Subject to constitutive nuclear export. - Information by UniProt
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Database links
- Entrez Gene: 2167 Human
- Omim: 600434 Human
- SwissProt: P15090 Human
- Unigene: 391561 Human
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Alternative names
- 3T3-L1 lipid-binding protein antibody
- 422/aP2 antibody
- A-FABP antibody
see all
Images
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Overlay histogram showing THP1 cells stained with ab93945 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab93945, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in THP1 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
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Anti-FABP4 antibody [9B8D] (ab93945) at 1/500 dilution + FABP4-hIgGFc transfected HEK293 cell lysate
Predicted band size: 15 kDa
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab93945 has been referenced in 3 publications.
- Zimoch J et al. Polyisocyanopeptide hydrogels: A novel thermo-responsive hydrogel supporting pre-vascularization and the development of organotypic structures. Acta Biomater 70:129-139 (2018). PubMed: 29454158
- Lee R et al. Deficient Adipogenesis of Scleroderma Patient and Healthy African American Monocytes. Front Pharmacol 8:174 (2017). PubMed: 28420992
- Pan Y et al. Survival of tissue-resident memory T cells requires exogenous lipid uptake and metabolism. Nature 543:252-256 (2017). PubMed: 28219080