Recombinant
RabMAb

Recombinant Anti-FABP4 antibody [EPR3579] (ab92501)

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Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR3579] to FABP4
  • Suitable for: WB, ICC/IF, IHC-P
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-FABP4 antibody [EPR3579]
    See all FABP4 primary antibodies

  • Description

    Rabbit monoclonal [EPR3579] to FABP4

  • Host species

    Rabbit

  • Specificity

    Ab92501 may cross-react with FABP, FABP3 and FABP9 based on the blast alignments.

    The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.

  • Tested applications

    Suitable for: WB, ICC/IF, IHC-Pmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    A synthetic peptide corresponding to residues in Human FABP4.

  • Positive control

    • WB: Mouse brown adipose tissue, Mouse heart, Mouse kidney, Mouse lung, Human adipose tissue,Rat adipose tissue and fetal heart lysates; ICC/IF: Adipocytes and 3T3-L1 cells; IHC-P: human breast tissue.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Applications

Our Abpromise guarantee covers the use of ab92501 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Detects a band of approximately 15 kDa (predicted molecular weight: 15 kDa).
ICC/IF 1/50.

For unpurified use at 1/1000.
IHC-P 1/16000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.

Target

  • Function

    Lipid transport protein in adipocytes. Binds both long chain fatty acids and retinoic acid. Delivers long-chain fatty acids and retinoic acid to their cognate receptors in the nucleus.

  • Sequence similarities

    Belongs to the calycin superfamily. Fatty-acid binding protein (FABP) family.

  • Domain

    Forms a beta-barrel structure that accommodates hydrophobic ligands in its interior.

  • Cellular localization

    Cytoplasm. Nucleus. Depending on the nature of the ligand, a conformation change exposes a nuclear localization motif and the protein is transported into the nucleus. Subject to constitutive nuclear export.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • 3T3-L1 lipid-binding protein antibody
    • 422/aP2 antibody
    • A-FABP antibody
    • adipocyte antibody
    • Adipocyte lipid binding protein antibody
    • Adipocyte lipid-binding protein antibody
    • Adipocyte protein AP2 antibody
    • Adipocyte-type fatty acid-binding protein antibody
    • AFABP antibody
    • ALBP antibody
    • ALBP/Ap2 antibody
    • aP2 antibody
    • Epididymis secretory protein Li 104 antibody
    • FABP antibody
    • FABP4 antibody
    • FABP4_HUMAN antibody
    • Fatty acid binding protein 4 adipocyte antibody
    • Fatty acid binding protein 4 antibody
    • Fatty acid binding protein adipocyte antibody
    • Fatty acid-binding protein 4 antibody
    • Fatty acid-binding protein antibody
    • HEL S 104 antibody
    • Lbpl antibody
    • Myelin P2 protein homolog antibody
    • P15 antibody
    • P2 adipocyte protein antibody
    • Protein 422 antibody
    see all

Images

  • Western blot - Anti-FABP4 antibody [EPR3579] (ab92501)
    Western blot - Anti-FABP4 antibody [EPR3579] (ab92501)
    All lanes : Anti-FABP4 antibody [EPR3579] (ab92501) at 1/1000 dilution (Purified)

    Lane 1 : Mouse brown adipose tissue lysates
    Lane 2 : Mouse heart lysates
    Lane 3 : Mouse kidney lysates
    Lane 4 : Mouse lung lysates
    Lane 5 : Human adipose tissue lysates
    Lane 6 : Rat adipose tissue lysates

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 15 kDa
    Observed band size: 15 kDa



    FABP4 is abundantly expressed in adipose tissue and at a lower level in lung, heart, skin, kidney, liver and brain (PMID: 23143994).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FABP4 antibody [EPR3579] (ab92501)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FABP4 antibody [EPR3579] (ab92501)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast tissue sections labeling FABP4 with Purified ab92501 at 1/16,000 dilution (0.03 µg/ml). Heat mediated antigen retrieval was performed Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
  • Immunocytochemistry/ Immunofluorescence - Anti-FABP4 antibody [EPR3579] (ab92501)
    Immunocytochemistry/ Immunofluorescence - Anti-FABP4 antibody [EPR3579] (ab92501)

    Immunocytochemistry/ Immunofluorescence analysis of 3T3-L1 (Mouse embryonic fibroblast) differentiated for 6 days cells labeling FABP4 with Purifiedab92501 at 1/50 dilution (9.9 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

  • Immunocytochemistry/ Immunofluorescence - Anti-FABP4 antibody [EPR3579] (ab92501)
    Immunocytochemistry/ Immunofluorescence - Anti-FABP4 antibody [EPR3579] (ab92501)

    FABP4 (green) was detected using FABP4 primary antibody (unpurified ab92501; diluted 1/1000). Alpha tubulin (red) was detected using our mouse monoclonal (ab7291) antibody. Cells were imaged by confocal microscopy, using z-stack for adipocyte-like cells.

  • Western blot - Anti-FABP4 antibody [EPR3579] (ab92501)
    Western blot - Anti-FABP4 antibody [EPR3579] (ab92501)
    All lanes : Anti-FABP4 antibody [EPR3579] (ab92501) at 1/1000 dilution (unpurified)

    Lane 1 : Human adipose
    tissue lysate
    Lane 2 : Human fetal heart lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : goat anti-rabbit HRP at 1/2000 dilution

    Predicted band size: 15 kDa
    Observed band size: 15 kDa

References

Publishing research using ab92501? Please let us know so that we can cite the reference in this datasheet.

ab92501 has been referenced in 38 publications.

  • Sindhu S  et al. Enhanced Adipose Expression of Interferon Regulatory Factor (IRF)-5 Associates with the Signatures of Metabolic Inflammation in Diabetic Obese Patients. Cells 9:N/A (2020). PubMed: 32188105
  • Wang H  et al. Phytanic acid activates PPARa to promote beige adipogenic differentiation of preadipocytes. J Nutr Biochem 67:201-211 (2019). PubMed: 30951974
  • Deng T  et al. FABP4 silencing ameliorates hypoxia reoxygenation injury through the attenuation of endoplasmic reticulum stress-mediated apoptosis by activating PI3K/Akt pathway. Life Sci 224:149-156 (2019). PubMed: 30904493
  • Nakagawa K  et al. UBIAD1 Plays an Essential Role in the Survival of Pancreatic Acinar Cells. Int J Mol Sci 20:N/A (2019). PubMed: 31013667
  • Gao H  et al. Lipoatrophy and metabolic disturbance in mice with adipose-specific deletion of kindlin-2. JCI Insight 4:N/A (2019). PubMed: 31292295
View all Publications for this product

Customer reviews and Q&As

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1-2 of 2 Abreviews or Q&A

Western blot abreview for Anti-FABP4 antibody [EPR3579]

 Average
Abreviews
abreview image
Application
Western blot
Sample
Rat Tissue lysate - whole (fat)
Gel Running Conditions
Non-reduced Non-Denaturing (Native) (gel 12%)
Loading amount
10 µg
Specification
fat
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Read More

Abcam user community

Verified customer

Submitted Mar 31 2017

Western blot abreview for Anti-FABP4 antibody [EPR3579]

 Excellent
Abreviews
abreview image
Application
Western blot
Sample
Mouse Tissue lysate - whole (mouse liver)
Loading amount
30 µg
Specification
mouse liver
Gel Running Conditions
Reduced Denaturing (14%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Read More

Mr. Dongil Kim

Verified customer

Submitted Apr 08 2013

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