Recombinant Anti-FABP4 antibody [EPR3579] (ab92501)
- Datasheet
- References (34)
- Protocols
Overview
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Product name
Anti-FABP4 antibody [EPR3579]
See all FABP4 primary antibodies -
Description
Rabbit monoclonal [EPR3579] to FABP4 -
Host species
Rabbit -
Specificity
Ab92501 may cross-react with FABP, FABP3 and FABP9 based on the blast alignments.
The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.
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Tested applications
Suitable for: WB, ICC/IF, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
A synthetic peptide corresponding to residues in Human FABP4.
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Positive control
- WB: Mouse brown adipose tissue, Mouse heart, Mouse kidney, Mouse lung, Human adipose tissue,Rat adipose tissue and fetal heart lysates; ICC/IF: Adipocytes and 3T3-L1 cells; IHC-P: human breast tissue.
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
This product is a recombinant rabbit monoclonal antibody.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR3579 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab92501 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/5000. Detects a band of approximately 15 kDa (predicted molecular weight: 15 kDa). | |
| ICC/IF | 1/50. For unpurified use at 1/1000. |
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| IHC-P | 1/16000. See IHC antigen retrieval protocols. The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat. |
Target
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Function
Lipid transport protein in adipocytes. Binds both long chain fatty acids and retinoic acid. Delivers long-chain fatty acids and retinoic acid to their cognate receptors in the nucleus. -
Sequence similarities
Belongs to the calycin superfamily. Fatty-acid binding protein (FABP) family. -
Domain
Forms a beta-barrel structure that accommodates hydrophobic ligands in its interior. -
Cellular localization
Cytoplasm. Nucleus. Depending on the nature of the ligand, a conformation change exposes a nuclear localization motif and the protein is transported into the nucleus. Subject to constitutive nuclear export. - Information by UniProt
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Database links
- Entrez Gene: 2167 Human
- Entrez Gene: 11770 Mouse
- Entrez Gene: 79451 Rat
- Omim: 600434 Human
- SwissProt: P15090 Human
- SwissProt: P04117 Mouse
- SwissProt: P70623 Rat
- Unigene: 391561 Human
see all -
Alternative names
- 3T3-L1 lipid-binding protein antibody
- 422/aP2 antibody
- A-FABP antibody
see all
Images
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![Western blot - Anti-FABP4 antibody [EPR3579] (ab92501)](https://www.abcam.com/ps/products/92/ab92501/Images/ab92501-352514-anti-fabp4-antibody-epr3579-westernblot-human-mouse-rat.jpg)
Western blot - Anti-FABP4 antibody [EPR3579] (ab92501)All lanes : Anti-FABP4 antibody [EPR3579] (ab92501) at 1/1000 dilution (Purified)
Lane 1 : Mouse brown adipose tissue lysates
Lane 2 : Mouse heart lysates
Lane 3 : Mouse kidney lysates
Lane 4 : Mouse lung lysates
Lane 5 : Human adipose tissue lysates
Lane 6 : Rat adipose tissue lysates
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 15 kDa
Observed band size: 15 kDaFABP4 is abundantly expressed in adipose tissue and at a lower level in lung, heart, skin, kidney, liver and brain (PMID: 23143994).
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FABP4 antibody [EPR3579] (ab92501)](https://www.abcam.com/ps/products/92/ab92501/Images/ab92501-4-anti-fabp4-antibody-epr3579-immunohistochemistry-breast-human.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FABP4 antibody [EPR3579] (ab92501)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast tissue sections labeling FABP4 with Purified ab92501 at 1/16,000 dilution (0.03 µg/ml). Heat mediated antigen retrieval was performed Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain. -
![Immunocytochemistry/ Immunofluorescence - Anti-FABP4 antibody [EPR3579] (ab92501)](https://www.abcam.com/ps/products/92/ab92501/Images/ab92501-5-anti-fabp4-antibody-epr3579-immunocytochemistry-3t3l1-mouse.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-FABP4 antibody [EPR3579] (ab92501)Immunocytochemistry/ Immunofluorescence analysis of 3T3-L1 (Mouse embryonic fibroblast) differentiated for 6 days cells labeling FABP4 with Purifiedab92501 at 1/50 dilution (9.9 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
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![Immunocytochemistry/ Immunofluorescence - Anti-FABP4 antibody [EPR3579] (ab92501)](https://www.abcam.com/ps/products/92/ab92501/Images/ab92501-250591-anti-fabp4-antibody-epr3579-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-FABP4 antibody [EPR3579] (ab92501)FABP4 (green) was detected using FABP4 primary antibody (unpurified ab92501; diluted 1/1000). Alpha tubulin (red) was detected using our mouse monoclonal (ab7291) antibody. Cells were imaged by confocal microscopy, using z-stack for adipocyte-like cells.
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![Western blot - Anti-FABP4 antibody [EPR3579] (ab92501)](https://www.abcam.com/ps/products/92/ab92501/Images/ab92501-107557-anti-fabp4-antibody-epr3579-western-blot.jpg)
Western blot - Anti-FABP4 antibody [EPR3579] (ab92501)All lanes : Anti-FABP4 antibody [EPR3579] (ab92501) at 1/1000 dilution (unpurified)
Lane 1 : Human adipose
tissue lysate
Lane 2 : Human fetal heart lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 15 kDa
Observed band size: 15 kDa
Protocols
References
This product has been referenced in:
- Wang H et al. Phytanic acid activates PPARa to promote beige adipogenic differentiation of preadipocytes. J Nutr Biochem 67:201-211 (2019). Read more (PubMed: 30951974) »
- Deng T et al. FABP4 silencing ameliorates hypoxia reoxygenation injury through the attenuation of endoplasmic reticulum stress-mediated apoptosis by activating PI3K/Akt pathway. Life Sci 224:149-156 (2019). Read more (PubMed: 30904493) »
