Overview

  • Product name

  • Description

    Rabbit polyclonal to FABP5
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, ELISA, WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant full length protein corresponding to Human FABP5.

  • General notes

     This product was previously labelled as Fatty Acid Binding Protein 5

     

Properties

Applications

Our Abpromise guarantee covers the use of ab37267 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 1 - 5 µg/ml.
ELISA Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Predicted molecular weight: 15 kDa.

Target

  • Function

    High specificity for fatty acids. Highest affinity for C18 chain length. Decreasing the chain length or introducing double bonds reduces the affinity. May be involved in keratinocyte differentiation.
  • Tissue specificity

    Keratinocytes; highly expressed in psoriatic skin.
  • Sequence similarities

    Belongs to the calycin superfamily. Fatty-acid binding protein (FABP) family.
  • Domain

    Forms a beta-barrel structure that accommodates the hydrophobic ligand in its interior.
  • Cellular localization

    Cytoplasm.
  • Information by UniProt
  • Database links

  • Alternative names

    • CFABP antibody
    • Cutaneous fatty acid binding protein antibody
    • DA11 antibody
    • Differentiation associated lipid binding protein LP2 antibody
    • E-FABP antibody
    • EFABP antibody
    • Epidermal-type fatty acid-binding protein antibody
    • FABP5 antibody
    • FABP5_HUMAN antibody
    • fatty acid binding protein 5 (psoriasis-associated) antibody
    • Fatty acid binding protein epidermal antibody
    • Fatty acid-binding protein 5 antibody
    • Fatty acid-binding protein, epidermal antibody
    • Keratinocyte lipid binding protein antibody
    • KFABP antibody
    • Klbp antibody
    • mal1 antibody
    • PA-FABP antibody
    • PAFABP antibody
    • Psoriasis associated fatty acid binding protein homolog antibody
    • Psoriasis-associated fatty acid-binding protein homolog antibody
    see all

Images

  • ab37267 at 1/200 dilution detecting FABP5 in human peripheral blood mononuclear cells. Goat anti-rabbit HRP was used as secondary antibody.

    See Abreview

  • ab37267 (1µg/mL) staining FABP5 in human oesophagus using an automated system (DAKO Autostainer Plus). Using this protocol there is strong staining of the cytoplasm and nuclei in stratified squamous epithelium cells.
    Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffers EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.

References

This product has been referenced in:

  • Vidotto A  et al. Differentially expressed proteins in positive versus negative HNSCC lymph nodes. BMC Med Genomics 11:73 (2018). Read more (PubMed: 30157864) »
  • Ozaki R  et al. Reprogramming of the retinoic acid pathway in decidualizing human endometrial stromal cells. PLoS One 12:e0173035 (2017). WB . Read more (PubMed: 28253328) »
See all 5 Publications for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Question
Answer

Thanks for your call today and for letting us know about the problem with this antibody concentration. As we discussed, I am sending free of charge replacement vials of ab37267. 7 vials are in stock and will arrive Monday on the order ***, and 1 vial is being transferred from our UK office and should arrive in about a week on the order ***. If you have any data regarding this issue, please forward it to me for our investigation. I look forward to hearing from you. Please let me know if you have any questions or if there is anything else that we can do for you.

Read More
Application
Western blot
Sample
Human Tissue lysate - whole (Peripheral blood mononuclear cells)
Loading amount
20 µg
Specification
Peripheral blood mononuclear cells
Gel Running Conditions
Non-reduced Denaturing (12% Tris-HEPES-SDS)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C

Abcam user community

Verified customer

Submitted Mar 09 2009

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