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Cardiovascular Lipids / Lipoproteins Fatty Acids Binding proteins
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Anti-FABP5 antibody (ab37267)

  • Datasheet
Reviews (1)Q&A (1)References (5)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FABP5 antibody (ab37267)

    Key features and details

    • Rabbit polyclonal to FABP5
    • Suitable for: IHC-P
    • Reacts with: Human
    • Isotype: IgG

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    Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    Protein
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    Recombinant Human FABP5 protein (ab83027)

    View more associated products

    Overview

    • Product name

      Anti-FABP5 antibody
      See all FABP5 primary antibodies
    • Description

      Rabbit polyclonal to FABP5
    • Host species

      Rabbit
    • Tested applications

      Suitable for: IHC-Pmore details
    • Species reactivity

      Reacts with: Human
    • Immunogen

      Recombinant full length protein corresponding to Human FABP5.

    • General notes

       This product was previously labelled as Fatty Acid Binding Protein 5

       

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
    • Storage buffer

      pH: 7.20
      Constituents: 0.58% Sodium chloride, 0.134% PBS
    • Concentration information loading...
    • Purity

      Immunogen affinity purified
    • Purification notes

      Immunoaffinity chromatography on a column with immobilized recombinant human FABP5.
    • Clonality

      Polyclonal
    • Isotype

      IgG
    • Research areas

      • Cardiovascular
      • Lipids / Lipoproteins
      • Fatty Acids
      • Binding proteins
      • Signal Transduction
      • Metabolism
      • Lipid metabolism
      • Cardiovascular
      • Lipids / Lipoproteins
      • Fatty Acids
      • Metabolism
      • Metabolism
      • Pathways and Processes
      • Metabolic signaling pathways
      • Lipid and lipoprotein metabolism
      • Fatty acids
      • Metabolism
      • Types of disease
      • Cancer
      • Metabolism
      • Pathways and Processes
      • Redox metabolism
      • Fatty acid oxidation
      • Cardiovascular
      • Angiogenesis
      • Endothelial Cell Markers

    Associated products

    • Compatible Secondaries

      • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
      • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Isotype control

      • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
    • Recombinant Protein

      • Recombinant Human FABP5 protein (ab83027)

    Applications

    Our Abpromise guarantee covers the use of ab37267 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    IHC-P Use a concentration of 1 - 5 µg/ml.

    Target

    • Function

      High specificity for fatty acids. Highest affinity for C18 chain length. Decreasing the chain length or introducing double bonds reduces the affinity. May be involved in keratinocyte differentiation.
    • Tissue specificity

      Keratinocytes; highly expressed in psoriatic skin.
    • Sequence similarities

      Belongs to the calycin superfamily. Fatty-acid binding protein (FABP) family.
    • Domain

      Forms a beta-barrel structure that accommodates the hydrophobic ligand in its interior.
    • Cellular localization

      Cytoplasm.
    • Target information above from: UniProt accession Q01469 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 2171 Human
      • Omim: 605168 Human
      • SwissProt: Q01469 Human
      • Unigene: 408061 Human
      • Alternative names

        • CFABP antibody
        • Cutaneous fatty acid binding protein antibody
        • DA11 antibody
        • Differentiation associated lipid binding protein LP2 antibody
        • E-FABP antibody
        • EFABP antibody
        • Epidermal-type fatty acid-binding protein antibody
        • FABP5 antibody
        • FABP5_HUMAN antibody
        • fatty acid binding protein 5 (psoriasis-associated) antibody
        • Fatty acid binding protein epidermal antibody
        • Fatty acid-binding protein 5 antibody
        • Fatty acid-binding protein, epidermal antibody
        • Keratinocyte lipid binding protein antibody
        • KFABP antibody
        • Klbp antibody
        • mal1 antibody
        • PA-FABP antibody
        • PAFABP antibody
        • Psoriasis associated fatty acid binding protein homolog antibody
        • Psoriasis-associated fatty acid-binding protein homolog antibody
        see all

      Images

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FABP5 antibody (ab37267)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FABP5 antibody (ab37267)

        ab37267 (1µg/mL) staining FABP5 in human oesophagus using an automated system (DAKO Autostainer Plus). Using this protocol there is strong staining of the cytoplasm and nuclei in stratified squamous epithelium cells.
        Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffers EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.

      Protocols

      • Immunohistochemistry protocols

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet
    • References (5)

      Publishing research using ab37267? Please let us know so that we can cite the reference in this datasheet.

      ab37267 has been referenced in 5 publications.

      • Vidotto A  et al. Differentially expressed proteins in positive versus negative HNSCC lymph nodes. BMC Med Genomics 11:73 (2018). PubMed: 30157864
      • Ozaki R  et al. Reprogramming of the retinoic acid pathway in decidualizing human endometrial stromal cells. PLoS One 12:e0173035 (2017). WB . PubMed: 28253328
      • Zhao G  et al. Effect of FABP5 gene silencing on the proliferation, apoptosis and invasion of human gastric SGC-7901 cancer cells. Oncol Lett 14:4772-4778 (2017). PubMed: 29085478
      • Zhou N  et al. Abscisic-acid-induced cellular apoptosis and differentiation in glioma via the retinoid acid signaling pathway. Int J Cancer 138:1947-58 (2016). WB . PubMed: 26594836
      • Buers I  et al. TIP47, a lipid cargo protein involved in macrophage triglyceride metabolism. Arterioscler Thromb Vasc Biol 29:767-73 (2009). PubMed: 19286631

      Customer reviews and Q&As

      Show All Reviews Q&A
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      Western blot abreview for Anti-Fatty Acid Binding Protein 5 antibody

      Excellent
      Abreviews
      Abreviews
      abreview image
      Application
      Western blot
      Sample
      Human Tissue lysate - whole (Peripheral blood mononuclear cells)
      Loading amount
      20 µg
      Specification
      Peripheral blood mononuclear cells
      Gel Running Conditions
      Non-reduced Denaturing (12% Tris-HEPES-SDS)
      Blocking step
      Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
      Read More

      Abcam user community

      Verified customer

      Submitted Mar 09 2009

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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