Anti-FABP5 antibody (ab37267)
- Datasheet
- References (5)
- Protocols
Overview
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Product name
Anti-FABP5 antibody
See all FABP5 primary antibodies -
Description
Rabbit polyclonal to FABP5 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, ELISA, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant full length protein corresponding to Human FABP5.
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General notes
This product was previously labelled as Fatty Acid Binding Protein 5
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.20
Constituents: 0.58% Sodium chloride, 0.134% PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Immunoaffinity chromatography on a column with immobilized recombinant human FABP5. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab37267 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | Use a concentration of 1 - 5 µg/ml. | |
| ELISA | Use at an assay dependent concentration. | |
| WB | Use at an assay dependent concentration. Predicted molecular weight: 15 kDa. |
Target
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Function
High specificity for fatty acids. Highest affinity for C18 chain length. Decreasing the chain length or introducing double bonds reduces the affinity. May be involved in keratinocyte differentiation. -
Tissue specificity
Keratinocytes; highly expressed in psoriatic skin. -
Sequence similarities
Belongs to the calycin superfamily. Fatty-acid binding protein (FABP) family. -
Domain
Forms a beta-barrel structure that accommodates the hydrophobic ligand in its interior. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 2171 Human
- Omim: 605168 Human
- SwissProt: Q01469 Human
- Unigene: 408061 Human
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Alternative names
- CFABP antibody
- Cutaneous fatty acid binding protein antibody
- DA11 antibody
see all
Images
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Western blot - Anti-FABP5 antibody (ab37267)This image is courtesy of an anonymous Abreviewab37267 at 1/200 dilution detecting FABP5 in human peripheral blood mononuclear cells. Goat anti-rabbit HRP was used as secondary antibody.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FABP5 antibody (ab37267)ab37267 (1µg/mL) staining FABP5 in human oesophagus using an automated system (DAKO Autostainer Plus). Using this protocol there is strong staining of the cytoplasm and nuclei in stratified squamous epithelium cells.
Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffers EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
Datasheets and documents
References
This product has been referenced in:
- Vidotto A et al. Differentially expressed proteins in positive versus negative HNSCC lymph nodes. BMC Med Genomics 11:73 (2018). Read more (PubMed: 30157864) »
- Ozaki R et al. Reprogramming of the retinoic acid pathway in decidualizing human endometrial stromal cells. PLoS One 12:e0173035 (2017). WB . Read more (PubMed: 28253328) »
